Characterization of Escherichia coli causing cellulitis in broilers
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Characterization of Escherichia coli causing cellulitis in broilers. / Poulsen, Louise Ladefoged; Bisgaard, Magne; Jørgensen, Steffen Lynge; Dideriksen, Tommy; Pedersen, Jacob Roland; Christensen, Henrik.
I: Veterinary Microbiology, Bind 225, 2018, s. 72-78.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Characterization of Escherichia coli causing cellulitis in broilers
AU - Poulsen, Louise Ladefoged
AU - Bisgaard, Magne
AU - Jørgensen, Steffen Lynge
AU - Dideriksen, Tommy
AU - Pedersen, Jacob Roland
AU - Christensen, Henrik
PY - 2018
Y1 - 2018
N2 - The aim of the study was to investigate cellulitis caused by Escherichia coli which has been responsible for economic and welfare problems in Danish broiler production between 2014 and 2016. The study included 13 flocks with unusually high condemnation rates due to cellulitis during a period of approximately one year. From six flocks, 126 condemned carcasses were collected at a Danish slaughterhouse. Further 272 broilers dead on their own were collected on nine broiler farms from flocks with increased mortality and cellulitis (2 farms included both birds from the rearing period and broilers subsequently condemned). All broilers were subjected to post mortem investigation including bacteriology and 247 E. coli isolates were obtained in pure culture from typical lesions of cellulitis. Two-hundred-thirty six E. coli isolates were investigated by pulsed field gel electrophoresis for clonality and 21 selected strains representing major clones were subsequently multi locus sequence typed allowing comparison to sequence types (ST) in the databases. One dominating PFGE type (A) was found to cause cellulitis on all 13 flocks (67% of all isolates). The clone belonged to ST117, which is well described as a pathogen in poultry, and was the primary agent responsible for cellulitis. Whole genome sequencing of eight E. coli isolates confirmed the close genetic relationship between isolates from the outbreaks and showed the presence of genes predicted to encode for the autotransporter proteins aatA, pic and upaG, reported to be of importance for adhesion of E. coli to eukaryotic cells.
AB - The aim of the study was to investigate cellulitis caused by Escherichia coli which has been responsible for economic and welfare problems in Danish broiler production between 2014 and 2016. The study included 13 flocks with unusually high condemnation rates due to cellulitis during a period of approximately one year. From six flocks, 126 condemned carcasses were collected at a Danish slaughterhouse. Further 272 broilers dead on their own were collected on nine broiler farms from flocks with increased mortality and cellulitis (2 farms included both birds from the rearing period and broilers subsequently condemned). All broilers were subjected to post mortem investigation including bacteriology and 247 E. coli isolates were obtained in pure culture from typical lesions of cellulitis. Two-hundred-thirty six E. coli isolates were investigated by pulsed field gel electrophoresis for clonality and 21 selected strains representing major clones were subsequently multi locus sequence typed allowing comparison to sequence types (ST) in the databases. One dominating PFGE type (A) was found to cause cellulitis on all 13 flocks (67% of all isolates). The clone belonged to ST117, which is well described as a pathogen in poultry, and was the primary agent responsible for cellulitis. Whole genome sequencing of eight E. coli isolates confirmed the close genetic relationship between isolates from the outbreaks and showed the presence of genes predicted to encode for the autotransporter proteins aatA, pic and upaG, reported to be of importance for adhesion of E. coli to eukaryotic cells.
KW - aat
KW - MLST
KW - PFGE
KW - pic
KW - SNP
KW - ST117
KW - upaG
U2 - 10.1016/j.vetmic.2018.09.011
DO - 10.1016/j.vetmic.2018.09.011
M3 - Journal article
C2 - 30322537
AN - SCOPUS:85053842002
VL - 225
SP - 72
EP - 78
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
ER -
ID: 218182294