TY - JOUR

T1 - Classification of bisgaard’s taxa 14 and 32 and a taxon from kestrels demonstrating satellitic growth and proposal of spirabiliibacterium gen. nov., including the description of three species

T2 - spirabiliibacterium mucosae sp. nov., spirabiliibacterium pneumoniae sp. nov. and spirabiliibacterium falconis sp. nov.

AU - Bisgaard, Magne

AU - Christensen, Henrik

PY - 2021

Y1 - 2021

N2 - Avian Pasteurella-like organisms tentatively named taxon 14 of Bisgaard have been obtained from different lesions in birds including ducks, turkeys, pigeons, geese and peafowl. Taxon 32 of Bisgaard was first reported from lesions in pigeon hawks (Accipiter gentiles). The taxon isolated from kestrels (Falco tinnunculus) was V-factor dependent and originally reported as Haemophilus-like. The results of 16S rRNA gene sequence based phylogenetic analysis recently indicated that the taxa 14 and 32 and the kestrel taxon were located in a monophyletic group distantly related to [Pasteurella] testudinis with 92–93% 16S rRNA gene sequence similarity. Comparison of 41 conserved protein sequences confirmed the monophyletic nature of the three taxa. Partial rpoB gene sequencing of 43 strains of taxon 14, taxon 32 and the kestrel taxon showed a relationship between taxon 14 and 32 of 88.2–90.0% similarity. Within taxon 14, 93.3–100% similarity was found, whereas the two strains of taxon 32 showed 99.8% rpoB similarity. Sequencing of 16S rRNA genes of strains representing the rpoB diversity outlined showed more than 98% similarity within taxon 14 and 99.4% within taxon 32, while the kestrel strains showed 100% 16S rRNA gene sequence similarity. A new genus, Spirabiliibacterium gen. nov., is proposed to include taxon 14, taxon 32 and the kestrel taxon. Phenotypically, members of the genus Spirabiliibacterium can be separated from members of the genera Aggregatibacter, Avibacterium and Volucribacter by maltose, oxidase and methyl red, respectively. Two or more phenotypic characters separate members of the genus Spirabiliibacterium from members of the remaining 27 genera of the family Pasteurellaceae.The G+C content of DNA ranged from 42.9 to 51.2% (genome sequence) for members of the genus Spirabiliibacterium. The type strain of Spirabiliibacterium mucosae (taxon 14 of Bisgaard) is 20609/3T (=CCUG 16499T=DSM 111429T=HIM 913-3T). The type strain of Spirabiliibacterium pneumoniae is HPA106T (=CCUG 74731T=DSM 111430T). The type strain of Spirabiliibacterium falconis (kestrel taxon) is IPDH 2176T (=NCTC 11878T=CCUG 28587T).

AB - Avian Pasteurella-like organisms tentatively named taxon 14 of Bisgaard have been obtained from different lesions in birds including ducks, turkeys, pigeons, geese and peafowl. Taxon 32 of Bisgaard was first reported from lesions in pigeon hawks (Accipiter gentiles). The taxon isolated from kestrels (Falco tinnunculus) was V-factor dependent and originally reported as Haemophilus-like. The results of 16S rRNA gene sequence based phylogenetic analysis recently indicated that the taxa 14 and 32 and the kestrel taxon were located in a monophyletic group distantly related to [Pasteurella] testudinis with 92–93% 16S rRNA gene sequence similarity. Comparison of 41 conserved protein sequences confirmed the monophyletic nature of the three taxa. Partial rpoB gene sequencing of 43 strains of taxon 14, taxon 32 and the kestrel taxon showed a relationship between taxon 14 and 32 of 88.2–90.0% similarity. Within taxon 14, 93.3–100% similarity was found, whereas the two strains of taxon 32 showed 99.8% rpoB similarity. Sequencing of 16S rRNA genes of strains representing the rpoB diversity outlined showed more than 98% similarity within taxon 14 and 99.4% within taxon 32, while the kestrel strains showed 100% 16S rRNA gene sequence similarity. A new genus, Spirabiliibacterium gen. nov., is proposed to include taxon 14, taxon 32 and the kestrel taxon. Phenotypically, members of the genus Spirabiliibacterium can be separated from members of the genera Aggregatibacter, Avibacterium and Volucribacter by maltose, oxidase and methyl red, respectively. Two or more phenotypic characters separate members of the genus Spirabiliibacterium from members of the remaining 27 genera of the family Pasteurellaceae.The G+C content of DNA ranged from 42.9 to 51.2% (genome sequence) for members of the genus Spirabiliibacterium. The type strain of Spirabiliibacterium mucosae (taxon 14 of Bisgaard) is 20609/3T (=CCUG 16499T=DSM 111429T=HIM 913-3T). The type strain of Spirabiliibacterium pneumoniae is HPA106T (=CCUG 74731T=DSM 111430T). The type strain of Spirabiliibacterium falconis (kestrel taxon) is IPDH 2176T (=NCTC 11878T=CCUG 28587T).

KW - European sparrowhawk

KW - Mallard

KW - Pasteurellaceae

KW - Sinusitis

KW - Taxonomy

KW - Turkey

U2 - 10.1099/ijsem.0.004758

DO - 10.1099/ijsem.0.004758

M3 - Journal article

C2 - 33734954

AN - SCOPUS:85103325921

VL - 71

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 3

M1 - 004758

ER -