Comparative evaluation of infection methods and environmental factors on challenge success: Aeromonas salmonicida infection in vaccinated rainbow trout

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Standard

Comparative evaluation of infection methods and environmental factors on challenge success : Aeromonas salmonicida infection in vaccinated rainbow trout. / Chettri, Jiwan Kumar; Skov, Jakob; Mohammad, Rezkar Jaafar; Krossøy, Bjørn; Kania, Per Walter; Dalsgaard, Inger; Buchmann, Kurt.

I: Fish and Shellfish Immunology, Bind 44, Nr. 2, 2015, s. 485-495.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Chettri, JK, Skov, J, Mohammad, RJ, Krossøy, B, Kania, PW, Dalsgaard, I & Buchmann, K 2015, 'Comparative evaluation of infection methods and environmental factors on challenge success: Aeromonas salmonicida infection in vaccinated rainbow trout', Fish and Shellfish Immunology, bind 44, nr. 2, s. 485-495. https://doi.org/10.1016/j.fsi.2015.03.003

APA

Chettri, J. K., Skov, J., Mohammad, R. J., Krossøy, B., Kania, P. W., Dalsgaard, I., & Buchmann, K. (2015). Comparative evaluation of infection methods and environmental factors on challenge success: Aeromonas salmonicida infection in vaccinated rainbow trout. Fish and Shellfish Immunology, 44(2), 485-495. https://doi.org/10.1016/j.fsi.2015.03.003

Vancouver

Chettri JK, Skov J, Mohammad RJ, Krossøy B, Kania PW, Dalsgaard I o.a. Comparative evaluation of infection methods and environmental factors on challenge success: Aeromonas salmonicida infection in vaccinated rainbow trout. Fish and Shellfish Immunology. 2015;44(2):485-495. https://doi.org/10.1016/j.fsi.2015.03.003

Author

Chettri, Jiwan Kumar ; Skov, Jakob ; Mohammad, Rezkar Jaafar ; Krossøy, Bjørn ; Kania, Per Walter ; Dalsgaard, Inger ; Buchmann, Kurt. / Comparative evaluation of infection methods and environmental factors on challenge success : Aeromonas salmonicida infection in vaccinated rainbow trout. I: Fish and Shellfish Immunology. 2015 ; Bind 44, Nr. 2. s. 485-495.

Bibtex

@article{13c2fde3dd2b4e73ad2fb530fdbd8196,
title = "Comparative evaluation of infection methods and environmental factors on challenge success: Aeromonas salmonicida infection in vaccinated rainbow trout",
abstract = "When testing vaccine-induced protection an effective and reliable challenge method is a basic requirement and we here present a comparative study on different challenge methods used for infection of rainbow trout Oncorhynchus mykiss with Aeromonas salmonicida, a bacterial pathogen eliciting furunculosis. Fish were vaccinated with three different adjuvanted trivalent vaccines containing formalin killed A. salmonicida, Vibrio anguillarum O1 and O2a. These were 1) the commercial vaccine Alpha Ject 3000, 2) an experimental vaccine with water in paraffin oil adjuvant, 3) an experimental vaccine with water in paraffin oil in water adjuvant. Fish were then exposed to A. salmonicida challenge using i.p. injection, cohabitation in freshwater, cohabitation in saltwater (15 ppt) or combined fresh/saltwater cohabitation. Cohabitation reflects a more natural infection mode and was shown to give better differentiation of vaccine types compared to i.p. injection of live bacteria. The latter infection mode is less successful probably due to the intra-abdominal inflammatory reactions (characterized in this study according to the Speilberg scale) induced by i.p. vaccination whereby injected live bacteria more effectively become inactivated at the site of injection. Compared to cohabitation in freshwater, cohabitation in saltwater was less efficient probably due to reduced survivability of A. salmonicida in saltwater, which was also experimentally verified in vitro.",
author = "Chettri, {Jiwan Kumar} and Jakob Skov and Mohammad, {Rezkar Jaafar} and Bj{\o}rn Kross{\o}y and Kania, {Per Walter} and Inger Dalsgaard and Kurt Buchmann",
year = "2015",
doi = "10.1016/j.fsi.2015.03.003",
language = "English",
volume = "44",
pages = "485--495",
journal = "Fish and Shellfish Immunology",
issn = "1050-4648",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Comparative evaluation of infection methods and environmental factors on challenge success

T2 - Aeromonas salmonicida infection in vaccinated rainbow trout

AU - Chettri, Jiwan Kumar

AU - Skov, Jakob

AU - Mohammad, Rezkar Jaafar

AU - Krossøy, Bjørn

AU - Kania, Per Walter

AU - Dalsgaard, Inger

AU - Buchmann, Kurt

PY - 2015

Y1 - 2015

N2 - When testing vaccine-induced protection an effective and reliable challenge method is a basic requirement and we here present a comparative study on different challenge methods used for infection of rainbow trout Oncorhynchus mykiss with Aeromonas salmonicida, a bacterial pathogen eliciting furunculosis. Fish were vaccinated with three different adjuvanted trivalent vaccines containing formalin killed A. salmonicida, Vibrio anguillarum O1 and O2a. These were 1) the commercial vaccine Alpha Ject 3000, 2) an experimental vaccine with water in paraffin oil adjuvant, 3) an experimental vaccine with water in paraffin oil in water adjuvant. Fish were then exposed to A. salmonicida challenge using i.p. injection, cohabitation in freshwater, cohabitation in saltwater (15 ppt) or combined fresh/saltwater cohabitation. Cohabitation reflects a more natural infection mode and was shown to give better differentiation of vaccine types compared to i.p. injection of live bacteria. The latter infection mode is less successful probably due to the intra-abdominal inflammatory reactions (characterized in this study according to the Speilberg scale) induced by i.p. vaccination whereby injected live bacteria more effectively become inactivated at the site of injection. Compared to cohabitation in freshwater, cohabitation in saltwater was less efficient probably due to reduced survivability of A. salmonicida in saltwater, which was also experimentally verified in vitro.

AB - When testing vaccine-induced protection an effective and reliable challenge method is a basic requirement and we here present a comparative study on different challenge methods used for infection of rainbow trout Oncorhynchus mykiss with Aeromonas salmonicida, a bacterial pathogen eliciting furunculosis. Fish were vaccinated with three different adjuvanted trivalent vaccines containing formalin killed A. salmonicida, Vibrio anguillarum O1 and O2a. These were 1) the commercial vaccine Alpha Ject 3000, 2) an experimental vaccine with water in paraffin oil adjuvant, 3) an experimental vaccine with water in paraffin oil in water adjuvant. Fish were then exposed to A. salmonicida challenge using i.p. injection, cohabitation in freshwater, cohabitation in saltwater (15 ppt) or combined fresh/saltwater cohabitation. Cohabitation reflects a more natural infection mode and was shown to give better differentiation of vaccine types compared to i.p. injection of live bacteria. The latter infection mode is less successful probably due to the intra-abdominal inflammatory reactions (characterized in this study according to the Speilberg scale) induced by i.p. vaccination whereby injected live bacteria more effectively become inactivated at the site of injection. Compared to cohabitation in freshwater, cohabitation in saltwater was less efficient probably due to reduced survivability of A. salmonicida in saltwater, which was also experimentally verified in vitro.

U2 - 10.1016/j.fsi.2015.03.003

DO - 10.1016/j.fsi.2015.03.003

M3 - Journal article

C2 - 25783001

VL - 44

SP - 485

EP - 495

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

IS - 2

ER -

ID: 135150939