Comparative host specificity of human- and pig-associated Staphylococcus aureus clonal lineages
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Comparative host specificity of human- and pig-associated Staphylococcus aureus clonal lineages. / Moodley, Arshnee; Espinosa-Gongora, Carmen ; Nielsen, Søren Saxmose; McCarthy, Alex J.; Lindsay, Jodi A.; Guardabassi, Luca.
I: P L o S One, Bind 7, Nr. 11, 2012.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Comparative host specificity of human- and pig-associated Staphylococcus aureus clonal lineages
AU - Moodley, Arshnee
AU - Espinosa-Gongora, Carmen
AU - Nielsen, Søren Saxmose
AU - McCarthy, Alex J.
AU - Lindsay, Jodi A.
AU - Guardabassi, Luca
N1 - e49344
PY - 2012
Y1 - 2012
N2 - Bacterial adhesion is a crucial step in colonization of the skin. In this study, we investigated the differential adherence to human and pig corneocytes of six Staphylococcus aureus strains belonging to three human-associated [ST8 (CC8), ST22 (CC22) and ST36(CC30)] and two pig-associated [ST398 (CC398) and ST433(CC30)] clonal lineages, and their colonization potential in the pig host was assessed by in vivo competition experiments. Corneocytes were collected from 11 humans and 21 pigs using D-squame® adhesive discs, and bacterial adherence to corneocytes was quantified by a standardized light microscopy assay. A previously described porcine colonization model was used to assess the potential of the six strains to colonize the pig host. Three pregnant, S. aureus-free sows were inoculated intravaginally shortly before farrowing with different strain mixes [mix 1) human and porcine ST398; mix 2) human ST36 and porcine ST433; and mix 3) human ST8, ST22, ST36 and porcine ST398] and the ability of individual strains to colonize the nasal cavity of newborn piglets was evaluated for 28 days after birth by strain-specific antibiotic selective culture. In the corneocyte assay, the pig-associated ST433 strain and the human-associated ST22 and ST36 strains showed significantly greater adhesion to porcine and human corneocytes, respectively (p
AB - Bacterial adhesion is a crucial step in colonization of the skin. In this study, we investigated the differential adherence to human and pig corneocytes of six Staphylococcus aureus strains belonging to three human-associated [ST8 (CC8), ST22 (CC22) and ST36(CC30)] and two pig-associated [ST398 (CC398) and ST433(CC30)] clonal lineages, and their colonization potential in the pig host was assessed by in vivo competition experiments. Corneocytes were collected from 11 humans and 21 pigs using D-squame® adhesive discs, and bacterial adherence to corneocytes was quantified by a standardized light microscopy assay. A previously described porcine colonization model was used to assess the potential of the six strains to colonize the pig host. Three pregnant, S. aureus-free sows were inoculated intravaginally shortly before farrowing with different strain mixes [mix 1) human and porcine ST398; mix 2) human ST36 and porcine ST433; and mix 3) human ST8, ST22, ST36 and porcine ST398] and the ability of individual strains to colonize the nasal cavity of newborn piglets was evaluated for 28 days after birth by strain-specific antibiotic selective culture. In the corneocyte assay, the pig-associated ST433 strain and the human-associated ST22 and ST36 strains showed significantly greater adhesion to porcine and human corneocytes, respectively (p
U2 - 10.1371/journal.pone.0049344
DO - 10.1371/journal.pone.0049344
M3 - Journal article
C2 - 23166643
VL - 7
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 11
ER -
ID: 43833490