Comparison of innate and Th1-type host immune responses in Oesophagostomum dentatum and Trichuris suis infections in pigs
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Comparison of innate and Th1-type host immune responses in Oesophagostomum dentatum and Trichuris suis infections in pigs. / Andreasen, A.; Skovgaard, K.; Klaver, E. J.; Van Die, I.; Mejer, H.; Thamsborg, S. M.; Kringel, H.
I: Parasite Immunology, Bind 38, Nr. 1, 01.2016, s. 53-63.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Comparison of innate and Th1-type host immune responses in Oesophagostomum dentatum and Trichuris suis infections in pigs
AU - Andreasen, A.
AU - Skovgaard, K.
AU - Klaver, E. J.
AU - Van Die, I.
AU - Mejer, H.
AU - Thamsborg, S. M.
AU - Kringel, H.
PY - 2016/1
Y1 - 2016/1
N2 - This study investigated the details of the innate and Th1/Treg-type-associated host immune responses in Trichuris suis and Oesophagostomum dentatum mono-and co-infected pigs and in vitro in stimulated porcine dendritic cell cultures. Forty-eight pigs were allocated into a 2-factorial design with two groups trickle-inoculated with 10 T. suis eggs/kg/day (Group T) or 20 O. dentatum L3/kg/day (O). Another group (OT) was infected with both parasites. Group C remained uninfected. Expression of innate and Th1/Treg-cell-associated genes in gut mucosa and associated lymph nodes was determined by qPCR at necropsy day 35 and 71. Gene expression showed suppressed/inhibited Th1 and Treg-type immune reactions, in accordance with previous findings of a predominant Th2-type immune response to both nematodes. The in vitro part examined the production of TNF-alpha in porcine dendritic cells (DC) exposed to T. suis and/or O. dentatum excretory/secretory (E/S) products. Further, binding capacity and structure of E/S products were characterized. Glycan and lectin-binding capacity were generally lower in O. dentatum E/S products compared to T. suis which may explain the earlier found weaker Th2 response to the former. Surprisingly, O. dentatum E/S products induced a significant (P < 0.0001) increase in TNF-alpha DC production, potentially indicating a new mode of helminth-host immune response interaction.
AB - This study investigated the details of the innate and Th1/Treg-type-associated host immune responses in Trichuris suis and Oesophagostomum dentatum mono-and co-infected pigs and in vitro in stimulated porcine dendritic cell cultures. Forty-eight pigs were allocated into a 2-factorial design with two groups trickle-inoculated with 10 T. suis eggs/kg/day (Group T) or 20 O. dentatum L3/kg/day (O). Another group (OT) was infected with both parasites. Group C remained uninfected. Expression of innate and Th1/Treg-cell-associated genes in gut mucosa and associated lymph nodes was determined by qPCR at necropsy day 35 and 71. Gene expression showed suppressed/inhibited Th1 and Treg-type immune reactions, in accordance with previous findings of a predominant Th2-type immune response to both nematodes. The in vitro part examined the production of TNF-alpha in porcine dendritic cells (DC) exposed to T. suis and/or O. dentatum excretory/secretory (E/S) products. Further, binding capacity and structure of E/S products were characterized. Glycan and lectin-binding capacity were generally lower in O. dentatum E/S products compared to T. suis which may explain the earlier found weaker Th2 response to the former. Surprisingly, O. dentatum E/S products induced a significant (P < 0.0001) increase in TNF-alpha DC production, potentially indicating a new mode of helminth-host immune response interaction.
KW - dendritic cells
KW - immune response
KW - Oesophagostomum dentatum
KW - pig
KW - qPCR
KW - Trichuris suis
U2 - 10.1111/pim.12296
DO - 10.1111/pim.12296
M3 - Journal article
C2 - 26609699
VL - 38
SP - 53
EP - 63
JO - Parasite Immunology
JF - Parasite Immunology
SN - 0141-9838
IS - 1
ER -
ID: 165438761