Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs

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Standard

Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs. / Goecke, Nicole B.; Hjulsager, Charlotte K.; Krog, Jesper S.; Skovgaard, Kerstin; Larsen, Lars E.

I: Journal of Veterinary Diagnostic Investigation, Bind 32, Nr. 1, 2020, s. 51-64.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Goecke, NB, Hjulsager, CK, Krog, JS, Skovgaard, K & Larsen, LE 2020, 'Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs', Journal of Veterinary Diagnostic Investigation, bind 32, nr. 1, s. 51-64. https://doi.org/10.1177/1040638719890863

APA

Goecke, N. B., Hjulsager, C. K., Krog, J. S., Skovgaard, K., & Larsen, L. E. (2020). Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs. Journal of Veterinary Diagnostic Investigation, 32(1), 51-64. https://doi.org/10.1177/1040638719890863

Vancouver

Goecke NB, Hjulsager CK, Krog JS, Skovgaard K, Larsen LE. Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs. Journal of Veterinary Diagnostic Investigation. 2020;32(1):51-64. https://doi.org/10.1177/1040638719890863

Author

Goecke, Nicole B. ; Hjulsager, Charlotte K. ; Krog, Jesper S. ; Skovgaard, Kerstin ; Larsen, Lars E. / Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs. I: Journal of Veterinary Diagnostic Investigation. 2020 ; Bind 32, Nr. 1. s. 51-64.

Bibtex

@article{077877cb3ea1405ba2ec1a558af0369e,
title = "Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs",
abstract = "Respiratory and intestinal diseases in pigs can have significant negative influence on productivity and animal welfare. A wide range of real-time PCR (rtPCR) assays are used in our laboratory (National Veterinary Institute, Technical University of Denmark) for pathogen detection, and PCR analyses are performed on traditional rtPCR platforms in which a limited number of samples can be analyzed per day given limitations in equipment and personnel. To mitigate these restrictions, rtPCR assays have been optimized for the high-throughput rtPCR BioMark platform (Fluidigm). Using this platform, we developed a high-throughput detection system that can be used for simultaneous examination of 48 samples with detection specificity for 18 selected respiratory and enteric viral and bacterial pathogens of high importance to Danish pig production. The rtPCR assays were validated and optimized to run under the same reaction conditions using a BioMark 48.48 dynamic array (DA) integrated fluidic circuit chip, and the sensitivity and specificity were assessed by testing known positive samples. Performance of the 48.48DA was similar to traditional rtPCR analysis, and the specificity of the 48.48DA was high. Application of the high-throughput platform has resulted in a significant reduction in cost and working hours and has provided production herds with a new innovative service with the potential to facilitate the optimal choice of disease control strategies such as vaccination and treatment.",
keywords = "Bacteria, detection, high-throughput, porcine pathogens, real-time PCR, viruses",
author = "Goecke, {Nicole B.} and Hjulsager, {Charlotte K.} and Krog, {Jesper S.} and Kerstin Skovgaard and Larsen, {Lars E.}",
year = "2020",
doi = "10.1177/1040638719890863",
language = "English",
volume = "32",
pages = "51--64",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "SAGE Publications",
number = "1",

}

RIS

TY - JOUR

T1 - Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs

AU - Goecke, Nicole B.

AU - Hjulsager, Charlotte K.

AU - Krog, Jesper S.

AU - Skovgaard, Kerstin

AU - Larsen, Lars E.

PY - 2020

Y1 - 2020

N2 - Respiratory and intestinal diseases in pigs can have significant negative influence on productivity and animal welfare. A wide range of real-time PCR (rtPCR) assays are used in our laboratory (National Veterinary Institute, Technical University of Denmark) for pathogen detection, and PCR analyses are performed on traditional rtPCR platforms in which a limited number of samples can be analyzed per day given limitations in equipment and personnel. To mitigate these restrictions, rtPCR assays have been optimized for the high-throughput rtPCR BioMark platform (Fluidigm). Using this platform, we developed a high-throughput detection system that can be used for simultaneous examination of 48 samples with detection specificity for 18 selected respiratory and enteric viral and bacterial pathogens of high importance to Danish pig production. The rtPCR assays were validated and optimized to run under the same reaction conditions using a BioMark 48.48 dynamic array (DA) integrated fluidic circuit chip, and the sensitivity and specificity were assessed by testing known positive samples. Performance of the 48.48DA was similar to traditional rtPCR analysis, and the specificity of the 48.48DA was high. Application of the high-throughput platform has resulted in a significant reduction in cost and working hours and has provided production herds with a new innovative service with the potential to facilitate the optimal choice of disease control strategies such as vaccination and treatment.

AB - Respiratory and intestinal diseases in pigs can have significant negative influence on productivity and animal welfare. A wide range of real-time PCR (rtPCR) assays are used in our laboratory (National Veterinary Institute, Technical University of Denmark) for pathogen detection, and PCR analyses are performed on traditional rtPCR platforms in which a limited number of samples can be analyzed per day given limitations in equipment and personnel. To mitigate these restrictions, rtPCR assays have been optimized for the high-throughput rtPCR BioMark platform (Fluidigm). Using this platform, we developed a high-throughput detection system that can be used for simultaneous examination of 48 samples with detection specificity for 18 selected respiratory and enteric viral and bacterial pathogens of high importance to Danish pig production. The rtPCR assays were validated and optimized to run under the same reaction conditions using a BioMark 48.48 dynamic array (DA) integrated fluidic circuit chip, and the sensitivity and specificity were assessed by testing known positive samples. Performance of the 48.48DA was similar to traditional rtPCR analysis, and the specificity of the 48.48DA was high. Application of the high-throughput platform has resulted in a significant reduction in cost and working hours and has provided production herds with a new innovative service with the potential to facilitate the optimal choice of disease control strategies such as vaccination and treatment.

KW - Bacteria

KW - detection

KW - high-throughput

KW - porcine pathogens

KW - real-time PCR

KW - viruses

U2 - 10.1177/1040638719890863

DO - 10.1177/1040638719890863

M3 - Journal article

C2 - 31752620

AN - SCOPUS:85075416666

VL - 32

SP - 51

EP - 64

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 1

ER -

ID: 247392678