Diverse bacteriophages for biocontrol of ESBL- and AmpC-β-lactamase-producing E. coli

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Diverse bacteriophages for biocontrol of ESBL- and AmpC-β-lactamase-producing E. coli. / Vitt, Amira R.; Sørensen, Anders Nørgaard; Bojer, Martin S.; Bortolaia, Valeria; Sørensen, Martine C. Holst; Brøndsted, Lone.

I: iScience, Bind 27, Nr. 2, 108826, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Vitt, AR, Sørensen, AN, Bojer, MS, Bortolaia, V, Sørensen, MCH & Brøndsted, L 2024, 'Diverse bacteriophages for biocontrol of ESBL- and AmpC-β-lactamase-producing E. coli', iScience, bind 27, nr. 2, 108826. https://doi.org/10.1016/j.isci.2024.108826

APA

Vitt, A. R., Sørensen, A. N., Bojer, M. S., Bortolaia, V., Sørensen, M. C. H., & Brøndsted, L. (2024). Diverse bacteriophages for biocontrol of ESBL- and AmpC-β-lactamase-producing E. coli. iScience, 27(2), [108826]. https://doi.org/10.1016/j.isci.2024.108826

Vancouver

Vitt AR, Sørensen AN, Bojer MS, Bortolaia V, Sørensen MCH, Brøndsted L. Diverse bacteriophages for biocontrol of ESBL- and AmpC-β-lactamase-producing E. coli. iScience. 2024;27(2). 108826. https://doi.org/10.1016/j.isci.2024.108826

Author

Vitt, Amira R. ; Sørensen, Anders Nørgaard ; Bojer, Martin S. ; Bortolaia, Valeria ; Sørensen, Martine C. Holst ; Brøndsted, Lone. / Diverse bacteriophages for biocontrol of ESBL- and AmpC-β-lactamase-producing E. coli. I: iScience. 2024 ; Bind 27, Nr. 2.

Bibtex

@article{1dd5c349fdfa4a1e90fc15329182bf07,
title = "Diverse bacteriophages for biocontrol of ESBL- and AmpC-β-lactamase-producing E. coli",
abstract = "Novel solutions are needed to reduce the risk of transmission of extended spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Escherichia coli (ESBL/AmpC E. coli) from livestock to humans. Given that phages are promising biocontrol agents, a collection of 28 phages that infect ESBL/AmpC E. coli were established. Whole genome sequencing showed that all these phages were unique and could be assigned to 15 different genera. Host range analysis showed that 82% of 198 strains, representing the genetic diversity of ESBL/AmpC E. coli, were sensitive to at least one phage. Identifying receptors used for phage binding experimentally as well as in silico predictions, allowed us to combine phages into two different cocktails with broad host range targeting diverse receptors. These phage cocktails efficiently inhibit the growth of ESBL/AmpC E. coli in vitro, thus suggesting the potential of phages as promising biocontrol agents.",
keywords = "Bacteriology, Biological sciences, Microbiology, Virology",
author = "Vitt, {Amira R.} and S{\o}rensen, {Anders N{\o}rgaard} and Bojer, {Martin S.} and Valeria Bortolaia and S{\o}rensen, {Martine C. Holst} and Lone Br{\o}ndsted",
note = "Publisher Copyright: {\textcopyright} 2024 The Author(s)",
year = "2024",
doi = "10.1016/j.isci.2024.108826",
language = "English",
volume = "27",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Diverse bacteriophages for biocontrol of ESBL- and AmpC-β-lactamase-producing E. coli

AU - Vitt, Amira R.

AU - Sørensen, Anders Nørgaard

AU - Bojer, Martin S.

AU - Bortolaia, Valeria

AU - Sørensen, Martine C. Holst

AU - Brøndsted, Lone

N1 - Publisher Copyright: © 2024 The Author(s)

PY - 2024

Y1 - 2024

N2 - Novel solutions are needed to reduce the risk of transmission of extended spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Escherichia coli (ESBL/AmpC E. coli) from livestock to humans. Given that phages are promising biocontrol agents, a collection of 28 phages that infect ESBL/AmpC E. coli were established. Whole genome sequencing showed that all these phages were unique and could be assigned to 15 different genera. Host range analysis showed that 82% of 198 strains, representing the genetic diversity of ESBL/AmpC E. coli, were sensitive to at least one phage. Identifying receptors used for phage binding experimentally as well as in silico predictions, allowed us to combine phages into two different cocktails with broad host range targeting diverse receptors. These phage cocktails efficiently inhibit the growth of ESBL/AmpC E. coli in vitro, thus suggesting the potential of phages as promising biocontrol agents.

AB - Novel solutions are needed to reduce the risk of transmission of extended spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Escherichia coli (ESBL/AmpC E. coli) from livestock to humans. Given that phages are promising biocontrol agents, a collection of 28 phages that infect ESBL/AmpC E. coli were established. Whole genome sequencing showed that all these phages were unique and could be assigned to 15 different genera. Host range analysis showed that 82% of 198 strains, representing the genetic diversity of ESBL/AmpC E. coli, were sensitive to at least one phage. Identifying receptors used for phage binding experimentally as well as in silico predictions, allowed us to combine phages into two different cocktails with broad host range targeting diverse receptors. These phage cocktails efficiently inhibit the growth of ESBL/AmpC E. coli in vitro, thus suggesting the potential of phages as promising biocontrol agents.

KW - Bacteriology

KW - Biological sciences

KW - Microbiology

KW - Virology

U2 - 10.1016/j.isci.2024.108826

DO - 10.1016/j.isci.2024.108826

M3 - Journal article

C2 - 38322997

AN - SCOPUS:85183488040

VL - 27

JO - iScience

JF - iScience

SN - 2589-0042

IS - 2

M1 - 108826

ER -

ID: 384497307