Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare
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Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare. / Jensen, Tanja L.; Kiersgaard, Maria K.; Mikkelsen, Lars F.; Sørensen, Dorte B.
I: Laboratory Animals, Bind 53, Nr. 6, 2019, s. 587-597.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Fasting of male mice – Effects of time point of initiation and duration on clinical chemistry parameters and animal welfare
AU - Jensen, Tanja L.
AU - Kiersgaard, Maria K.
AU - Mikkelsen, Lars F.
AU - Sørensen, Dorte B.
PY - 2019
Y1 - 2019
N2 - Fasting of mice is a common procedure, which can affect the outcome of the study as well as animal welfare. In this study, we assess the effects of fasting, fasting duration and fasting initiation time in relation to light schedule and present suggestions for optimization of fasting. Male C57BL/6NCrl mice were fasted for 0, 3, 6, 12, 18 and 24 hours initiated either in the light period (photophase) or the dark period (scotophase). Body weight, gastric content, body temperature, corticosterone and 19 routine clinical chemistry parameters were evaluated. Fasting caused significant changes in most of the measured parameters. Increasing duration of fasting resulted in increasing physiological changes. Fasting initiated in the scotophase caused more significant changes than fasting initiated in the photophase. To cause the least physiological changes in mice and increase animal welfare, mice should preferably be fasted in the photophase and for the shortest possible period allowed by the experimental purpose of fasting.
AB - Fasting of mice is a common procedure, which can affect the outcome of the study as well as animal welfare. In this study, we assess the effects of fasting, fasting duration and fasting initiation time in relation to light schedule and present suggestions for optimization of fasting. Male C57BL/6NCrl mice were fasted for 0, 3, 6, 12, 18 and 24 hours initiated either in the light period (photophase) or the dark period (scotophase). Body weight, gastric content, body temperature, corticosterone and 19 routine clinical chemistry parameters were evaluated. Fasting caused significant changes in most of the measured parameters. Increasing duration of fasting resulted in increasing physiological changes. Fasting initiated in the scotophase caused more significant changes than fasting initiated in the photophase. To cause the least physiological changes in mice and increase animal welfare, mice should preferably be fasted in the photophase and for the shortest possible period allowed by the experimental purpose of fasting.
KW - Fasting
KW - mouse
KW - refinement
KW - welfare
U2 - 10.1177/0023677218824373
DO - 10.1177/0023677218824373
M3 - Journal article
C2 - 30741083
AN - SCOPUS:85061611693
VL - 53
SP - 587
EP - 597
JO - Laboratory Animals
JF - Laboratory Animals
SN - 0023-6772
IS - 6
ER -
ID: 217946377