Fluorescent in-situ hybridization of cattle and sheep chromosomes with cloned human fragile-X DNA

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Standard

Fluorescent in-situ hybridization of cattle and sheep chromosomes with cloned human fragile-X DNA. / Ali, Ahmd; Thomsen, Preben Dybdahl; Babar, M.E.

I: Molecular Biology Reports, Bind 36, Nr. 3, 2009, s. 619-622.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ali, A, Thomsen, PD & Babar, ME 2009, 'Fluorescent in-situ hybridization of cattle and sheep chromosomes with cloned human fragile-X DNA', Molecular Biology Reports, bind 36, nr. 3, s. 619-622. https://doi.org/10.1007/s11033-008-9221-8

APA

Ali, A., Thomsen, P. D., & Babar, M. E. (2009). Fluorescent in-situ hybridization of cattle and sheep chromosomes with cloned human fragile-X DNA. Molecular Biology Reports, 36(3), 619-622. https://doi.org/10.1007/s11033-008-9221-8

Vancouver

Ali A, Thomsen PD, Babar ME. Fluorescent in-situ hybridization of cattle and sheep chromosomes with cloned human fragile-X DNA. Molecular Biology Reports. 2009;36(3):619-622. https://doi.org/10.1007/s11033-008-9221-8

Author

Ali, Ahmd ; Thomsen, Preben Dybdahl ; Babar, M.E. / Fluorescent in-situ hybridization of cattle and sheep chromosomes with cloned human fragile-X DNA. I: Molecular Biology Reports. 2009 ; Bind 36, Nr. 3. s. 619-622.

Bibtex

@article{78f16b309d2211debc73000ea68e967b,
title = "Fluorescent in-situ hybridization of cattle and sheep chromosomes with cloned human fragile-X DNA",
abstract = "An extensive study on spontaneous and 5-Fluorodeoxyuridine induced fragile sites identified Xq31 in cattle (Bos taurus) and (Xq24, Xq26) in sheep (Ovis aries) in addition to several autosomal fragile sites (under publication). A ZOO-FISH study using three cloned human fragile-X probes with CCG/CGG(n) trinucleotide repeat sequence was carried out to determine homology between human and bovine fragile-X. The hybridisation results showed only a weak signal on a human chromosome that was not an X with all three fragile site probes. No signals were detected in sheep chromosomes. The signal of all three human fragile-X probes on cattle chromosomes was however, medium-prominent sub-centromeric signal on two homologues. BrdU administration in 12 h before harvesting identified these homologues to be chromosome number 5. In addition retrospective slides of cattle and sheep chromosomes used for fragile site studies showed no signals whatsoever. It was therefore concluded that no homology existed between human and bovine fragile-X.",
author = "Ahmd Ali and Thomsen, {Preben Dybdahl} and M.E. Babar",
year = "2009",
doi = "10.1007/s11033-008-9221-8",
language = "English",
volume = "36",
pages = "619--622",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - Fluorescent in-situ hybridization of cattle and sheep chromosomes with cloned human fragile-X DNA

AU - Ali, Ahmd

AU - Thomsen, Preben Dybdahl

AU - Babar, M.E.

PY - 2009

Y1 - 2009

N2 - An extensive study on spontaneous and 5-Fluorodeoxyuridine induced fragile sites identified Xq31 in cattle (Bos taurus) and (Xq24, Xq26) in sheep (Ovis aries) in addition to several autosomal fragile sites (under publication). A ZOO-FISH study using three cloned human fragile-X probes with CCG/CGG(n) trinucleotide repeat sequence was carried out to determine homology between human and bovine fragile-X. The hybridisation results showed only a weak signal on a human chromosome that was not an X with all three fragile site probes. No signals were detected in sheep chromosomes. The signal of all three human fragile-X probes on cattle chromosomes was however, medium-prominent sub-centromeric signal on two homologues. BrdU administration in 12 h before harvesting identified these homologues to be chromosome number 5. In addition retrospective slides of cattle and sheep chromosomes used for fragile site studies showed no signals whatsoever. It was therefore concluded that no homology existed between human and bovine fragile-X.

AB - An extensive study on spontaneous and 5-Fluorodeoxyuridine induced fragile sites identified Xq31 in cattle (Bos taurus) and (Xq24, Xq26) in sheep (Ovis aries) in addition to several autosomal fragile sites (under publication). A ZOO-FISH study using three cloned human fragile-X probes with CCG/CGG(n) trinucleotide repeat sequence was carried out to determine homology between human and bovine fragile-X. The hybridisation results showed only a weak signal on a human chromosome that was not an X with all three fragile site probes. No signals were detected in sheep chromosomes. The signal of all three human fragile-X probes on cattle chromosomes was however, medium-prominent sub-centromeric signal on two homologues. BrdU administration in 12 h before harvesting identified these homologues to be chromosome number 5. In addition retrospective slides of cattle and sheep chromosomes used for fragile site studies showed no signals whatsoever. It was therefore concluded that no homology existed between human and bovine fragile-X.

U2 - 10.1007/s11033-008-9221-8

DO - 10.1007/s11033-008-9221-8

M3 - Journal article

C2 - 18340550

VL - 36

SP - 619

EP - 622

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 3

ER -

ID: 14303846