Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks

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Standard

Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks. / Blixenkrone-Møller, Merete; Martella, Vito.

I: Veterinary Microbiology, Bind 122, Nr. 1-2, 2007, s. 32-42.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Blixenkrone-Møller, M & Martella, V 2007, 'Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks', Veterinary Microbiology, bind 122, nr. 1-2, s. 32-42. https://doi.org/10.1016/j.vetmic.2007.01.005

APA

Blixenkrone-Møller, M., & Martella, V. (2007). Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks. Veterinary Microbiology, 122(1-2), 32-42. https://doi.org/10.1016/j.vetmic.2007.01.005

Vancouver

Blixenkrone-Møller M, Martella V. Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks. Veterinary Microbiology. 2007;122(1-2):32-42. https://doi.org/10.1016/j.vetmic.2007.01.005

Author

Blixenkrone-Møller, Merete ; Martella, Vito. / Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks. I: Veterinary Microbiology. 2007 ; Bind 122, Nr. 1-2. s. 32-42.

Bibtex

@article{9838f5a0a1c211ddb6ae000ea68e967b,
title = "Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks",
abstract = "CDV is a highly contagious viral pathogen causing a lethal systemic disease in dogs and other carnivores. Several lineages or genotypes of CDV exist that are variously distributed throughout several continents. Legal or uncontrolled trading of animals may modify the epidemiology of CDV, introducing novel strains in CDV-na{\"i}ve areas or accounting for the resurgence of CDV in areas where vaccine prophylaxis was effective and successful to control the disease. A hemi-nested PCR system was developed to genotype strains of the major CDV lineages, America-1, Europe, Asia-1, Asia-2 and Arctic. The assay was tested using a collection of 27 laboratory and vaccine strains and of 36 field CDV strains. Distinct lineages could be differentiated by specific primers targeted to the H gene. The method could be useful for molecular epidemiological studies of CDV, providing a tool for large-scale studies, and for the diagnosis of vaccine-related disease.",
keywords = "Former LIFE faculty, Hundesygevirus, genotypning, Canine distemper virus, genotyping",
author = "Merete Blixenkrone-M{\o}ller and Vito Martella",
year = "2007",
doi = "10.1016/j.vetmic.2007.01.005",
language = "English",
volume = "122",
pages = "32--42",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks

AU - Blixenkrone-Møller, Merete

AU - Martella, Vito

PY - 2007

Y1 - 2007

N2 - CDV is a highly contagious viral pathogen causing a lethal systemic disease in dogs and other carnivores. Several lineages or genotypes of CDV exist that are variously distributed throughout several continents. Legal or uncontrolled trading of animals may modify the epidemiology of CDV, introducing novel strains in CDV-naïve areas or accounting for the resurgence of CDV in areas where vaccine prophylaxis was effective and successful to control the disease. A hemi-nested PCR system was developed to genotype strains of the major CDV lineages, America-1, Europe, Asia-1, Asia-2 and Arctic. The assay was tested using a collection of 27 laboratory and vaccine strains and of 36 field CDV strains. Distinct lineages could be differentiated by specific primers targeted to the H gene. The method could be useful for molecular epidemiological studies of CDV, providing a tool for large-scale studies, and for the diagnosis of vaccine-related disease.

AB - CDV is a highly contagious viral pathogen causing a lethal systemic disease in dogs and other carnivores. Several lineages or genotypes of CDV exist that are variously distributed throughout several continents. Legal or uncontrolled trading of animals may modify the epidemiology of CDV, introducing novel strains in CDV-naïve areas or accounting for the resurgence of CDV in areas where vaccine prophylaxis was effective and successful to control the disease. A hemi-nested PCR system was developed to genotype strains of the major CDV lineages, America-1, Europe, Asia-1, Asia-2 and Arctic. The assay was tested using a collection of 27 laboratory and vaccine strains and of 36 field CDV strains. Distinct lineages could be differentiated by specific primers targeted to the H gene. The method could be useful for molecular epidemiological studies of CDV, providing a tool for large-scale studies, and for the diagnosis of vaccine-related disease.

KW - Former LIFE faculty

KW - Hundesygevirus, genotypning

KW - Canine distemper virus, genotyping

U2 - 10.1016/j.vetmic.2007.01.005

DO - 10.1016/j.vetmic.2007.01.005

M3 - Journal article

C2 - 17275219

VL - 122

SP - 32

EP - 42

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-2

ER -

ID: 8075420