Glaesserella Australis sp. nov., isolated from the lungs of pigs
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Glaesserella Australis sp. nov., isolated from the lungs of pigs. / Turni, Conny; Wu, Yunchen; Omaleki, Lida; Giang, Nhan; Blackall, P. J.; Christensen, Henrik.
I: International Journal of Systematic and Evolutionary Microbiology, Bind 70, Nr. 6, 004221, 2020, s. 3686-3692.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Glaesserella Australis sp. nov., isolated from the lungs of pigs
AU - Turni, Conny
AU - Wu, Yunchen
AU - Omaleki, Lida
AU - Giang, Nhan
AU - Blackall, P. J.
AU - Christensen, Henrik
PY - 2020
Y1 - 2020
N2 - Twenty-nine isolates of an unknown haemophilic organism were isolated from the lungs of pigs from 14 farms in Australia. Phylogenetic analyses based on the 16S rRNA gene, recN and rpoA showed a monophyletic group that was most closely related to Glaesserella parasuis and [Actinobacillus] indolicus. Whole genome sequence analysis indicated that the Glaesserella parasuis and this group, using the type strain HS4635T for comparison, showed a similarity of 30.9 % DNA–DNA renaturation. The isolates were Gram-stain-negative, NAD-dependent, CAMP-negative and were oxidase-positive, catalase-negative and produced indole but not urease. The isolates could be separated from all currently recognized haemophilic and non-haemophilic members of the family Pastuerellaceae. Key phenotypic properties were the production of indole, the lack of urease activity, production of β-galactosidase but not α-fucosidase, acid formation from (−)-d-arabinose, (+)-d-galactose, maltose and trehalose and a failure to produce acid from (−)-d-mannitol. Taken together, these data indicate that the isolates belong to a novel species for which the name Glaesserella australis sp. nov. is proposed. The type strain is HS4635T (=CCUG 71931T and LMG 30645T).
AB - Twenty-nine isolates of an unknown haemophilic organism were isolated from the lungs of pigs from 14 farms in Australia. Phylogenetic analyses based on the 16S rRNA gene, recN and rpoA showed a monophyletic group that was most closely related to Glaesserella parasuis and [Actinobacillus] indolicus. Whole genome sequence analysis indicated that the Glaesserella parasuis and this group, using the type strain HS4635T for comparison, showed a similarity of 30.9 % DNA–DNA renaturation. The isolates were Gram-stain-negative, NAD-dependent, CAMP-negative and were oxidase-positive, catalase-negative and produced indole but not urease. The isolates could be separated from all currently recognized haemophilic and non-haemophilic members of the family Pastuerellaceae. Key phenotypic properties were the production of indole, the lack of urease activity, production of β-galactosidase but not α-fucosidase, acid formation from (−)-d-arabinose, (+)-d-galactose, maltose and trehalose and a failure to produce acid from (−)-d-mannitol. Taken together, these data indicate that the isolates belong to a novel species for which the name Glaesserella australis sp. nov. is proposed. The type strain is HS4635T (=CCUG 71931T and LMG 30645T).
KW - Classification
KW - Identification
KW - Pasteurellaceae
KW - Pigs
U2 - 10.1099/ijsem.0.004221
DO - 10.1099/ijsem.0.004221
M3 - Journal article
C2 - 32416742
AN - SCOPUS:85087169601
VL - 70
SP - 3686
EP - 3692
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 6
M1 - 004221
ER -
ID: 244917908