High Fat Diet Triggers a Prompt and Transient Increase in Adipose Tissue Granulocyte Colony Stimulating Factor and Circulating MyeloidCells in Mice
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High Fat Diet Triggers a Prompt and Transient Increase in Adipose Tissue Granulocyte Colony Stimulating Factor and Circulating MyeloidCells in Mice. / Eld, Helene Marie Skovsted; Madsen, Louise; Lund, Christina H.; Metzdorff, Stine Broeng; Frøkiær, Hanne.
I: Asian Journal of Immunology, Bind 5, Nr. 4, AJI.83640, 2021, s. 7-21.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - High Fat Diet Triggers a Prompt and Transient Increase in Adipose Tissue Granulocyte Colony Stimulating Factor and Circulating MyeloidCells in Mice
AU - Eld, Helene Marie Skovsted
AU - Madsen, Louise
AU - Lund, Christina H.
AU - Metzdorff, Stine Broeng
AU - Frøkiær, Hanne
PY - 2021
Y1 - 2021
N2 - Scope: The short-term effects of feeding high fat diet (HFD) to mice was investigated with focus on the effect on myelopoesis, circulating neutrophils and the induction of Granulocyte colony stimulating factor (G-CSF).Methods: Male mice were fed HFD (45%) during a period of 5 weeks with samples taken after 3 days and 1, 3, 4 and 5 weeks. Blood was analyzed for neutrophils and monocytes, for G-CSF and granulocyte-macrophage (GM)-CSF, and for cytokine expression. Visceral adipose tissue (VAT) expression of various genes and production of G-GSF and GM-CSF in cultured VAT was determined.Results: Three days after commencement of HFD, the number of circulatory neutrophils and monocytes increased but returned to baseline-level at day 8. This transient increase coincided with an increased blood concentration of G-CSF and a transient increase in bone marrow and spleen neutrophils. In supernatant from cultivated visceral adipose tissue isolated from HFD fed mice on day 3 and 8, G-CSF was increased. The expression of Toll-like receptor 4 in adipose tissue was down-regulated from week 4. In vitro, lipopolysaccharide (LPS) was a poor stimulator of G-CSF, while G-CSF or LPS together with G-CSF or GM-CSF induced increased G-CSF production. G-CSF suppressed production of LPS-induced TNFa and increased IL-10 production in dendritic cells suggesting that G-CSF down-regulates LPS-induced inflammation.Conclusion: HFD induces a transient increase in adipose tissue G-GSF and circulating myeloid cells in mice. We suggest G-CSF induces increased myelopoiesis and simultaneously down-regulates LPS-induced inflammation.
AB - Scope: The short-term effects of feeding high fat diet (HFD) to mice was investigated with focus on the effect on myelopoesis, circulating neutrophils and the induction of Granulocyte colony stimulating factor (G-CSF).Methods: Male mice were fed HFD (45%) during a period of 5 weeks with samples taken after 3 days and 1, 3, 4 and 5 weeks. Blood was analyzed for neutrophils and monocytes, for G-CSF and granulocyte-macrophage (GM)-CSF, and for cytokine expression. Visceral adipose tissue (VAT) expression of various genes and production of G-GSF and GM-CSF in cultured VAT was determined.Results: Three days after commencement of HFD, the number of circulatory neutrophils and monocytes increased but returned to baseline-level at day 8. This transient increase coincided with an increased blood concentration of G-CSF and a transient increase in bone marrow and spleen neutrophils. In supernatant from cultivated visceral adipose tissue isolated from HFD fed mice on day 3 and 8, G-CSF was increased. The expression of Toll-like receptor 4 in adipose tissue was down-regulated from week 4. In vitro, lipopolysaccharide (LPS) was a poor stimulator of G-CSF, while G-CSF or LPS together with G-CSF or GM-CSF induced increased G-CSF production. G-CSF suppressed production of LPS-induced TNFa and increased IL-10 production in dendritic cells suggesting that G-CSF down-regulates LPS-induced inflammation.Conclusion: HFD induces a transient increase in adipose tissue G-GSF and circulating myeloid cells in mice. We suggest G-CSF induces increased myelopoiesis and simultaneously down-regulates LPS-induced inflammation.
M3 - Journal article
VL - 5
SP - 7
EP - 21
JO - Asian Journal of Immunology
JF - Asian Journal of Immunology
IS - 4
M1 - AJI.83640
ER -
ID: 320355490