High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos

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Standard

High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos. / Li, J.; Østrup, Olga; Villemoes, Klaus; Kragh, Peter Michael; Schmidt, Mette; Bøgh, Ingrid Brück; Zhang, Y.; Du, Yutao; Lin, L.; Purup, Stig; Xue, Q.; Bolund, Lars; Yang, H.; Maddox-Hyttel, Poul; Vajta, Gabor.

I: Theriogenology, Bind 70, Nr. 5, 2008, s. 800-808.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Li, J, Østrup, O, Villemoes, K, Kragh, PM, Schmidt, M, Bøgh, IB, Zhang, Y, Du, Y, Lin, L, Purup, S, Xue, Q, Bolund, L, Yang, H, Maddox-Hyttel, P & Vajta, G 2008, 'High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos', Theriogenology, bind 70, nr. 5, s. 800-808. https://doi.org/10.1016/j.theriogenology.2008.05.046

APA

Li, J., Østrup, O., Villemoes, K., Kragh, P. M., Schmidt, M., Bøgh, I. B., Zhang, Y., Du, Y., Lin, L., Purup, S., Xue, Q., Bolund, L., Yang, H., Maddox-Hyttel, P., & Vajta, G. (2008). High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos. Theriogenology, 70(5), 800-808. https://doi.org/10.1016/j.theriogenology.2008.05.046

Vancouver

Li J, Østrup O, Villemoes K, Kragh PM, Schmidt M, Bøgh IB o.a. High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos. Theriogenology. 2008;70(5):800-808. https://doi.org/10.1016/j.theriogenology.2008.05.046

Author

Li, J. ; Østrup, Olga ; Villemoes, Klaus ; Kragh, Peter Michael ; Schmidt, Mette ; Bøgh, Ingrid Brück ; Zhang, Y. ; Du, Yutao ; Lin, L. ; Purup, Stig ; Xue, Q. ; Bolund, Lars ; Yang, H. ; Maddox-Hyttel, Poul ; Vajta, Gabor. / High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos. I: Theriogenology. 2008 ; Bind 70, Nr. 5. s. 800-808.

Bibtex

@article{1d84d750a1c411ddb6ae000ea68e967b,
title = "High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos",
abstract = "Abnormal epigenetic modification is supposed to be one of factors accounting for inefficient reprogramming of the donor cell nuclei in ooplasm after somatic cell nuclear transfer (SCNT). Trichostatin A (TSA) is an inhibitor of histone deacetylase, potentially enhancing cloning efficiency. The aim of our present study was to establish the optimal TSA treatment in order to improve the development of handmade cloned (HMC) porcine embryos and examine the effect of TSA on their development. The blastocyst percentage of HMC embryos treated with 37.5 nM TSA for 22-24 h after activation increased up to 80% (control grpup-54%; P<0.05). TSA mediated increase in histone acetylation was proved by immunofluorescence analysis of acH3K9 and acH4K16. 2-cell stage embryos derived from TSA treatment displayed significant increase in histone acetylation compared to control embryos, whereas no significant differences were observed at blastocyst stage. During time-lapse monitoring, no difference was observed in the kinetics of 2-cell stage embryos. Compact morula (CM) stage was reached 15 h later in TSA treated embryos compared to the control. Blastocysts (Day 5 and 6) from HMC embryos treated with TSA were transferred to 2 recipients resulting in one pregnancy and birth of one live and five dead piglets. Our data demonstrate that TSA treatment after HMC in pigs may affect reprogramming of the somatic genome resulting in higher in vitro embryo development, and enable full-term in vivo development.",
keywords = "Former LIFE faculty, Pig, Handmade cloning, Histone acetylation, Time-lapse, Reprogramming",
author = "J. Li and Olga {\O}strup and Klaus Villemoes and Kragh, {Peter Michael} and Mette Schmidt and B{\o}gh, {Ingrid Br{\"u}ck} and Y. Zhang and Yutao Du and L. Lin and Stig Purup and Q. Xue and Lars Bolund and H. Yang and Poul Maddox-Hyttel and Gabor Vajta",
year = "2008",
doi = "10.1016/j.theriogenology.2008.05.046",
language = "English",
volume = "70",
pages = "800--808",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos

AU - Li, J.

AU - Østrup, Olga

AU - Villemoes, Klaus

AU - Kragh, Peter Michael

AU - Schmidt, Mette

AU - Bøgh, Ingrid Brück

AU - Zhang, Y.

AU - Du, Yutao

AU - Lin, L.

AU - Purup, Stig

AU - Xue, Q.

AU - Bolund, Lars

AU - Yang, H.

AU - Maddox-Hyttel, Poul

AU - Vajta, Gabor

PY - 2008

Y1 - 2008

N2 - Abnormal epigenetic modification is supposed to be one of factors accounting for inefficient reprogramming of the donor cell nuclei in ooplasm after somatic cell nuclear transfer (SCNT). Trichostatin A (TSA) is an inhibitor of histone deacetylase, potentially enhancing cloning efficiency. The aim of our present study was to establish the optimal TSA treatment in order to improve the development of handmade cloned (HMC) porcine embryos and examine the effect of TSA on their development. The blastocyst percentage of HMC embryos treated with 37.5 nM TSA for 22-24 h after activation increased up to 80% (control grpup-54%; P<0.05). TSA mediated increase in histone acetylation was proved by immunofluorescence analysis of acH3K9 and acH4K16. 2-cell stage embryos derived from TSA treatment displayed significant increase in histone acetylation compared to control embryos, whereas no significant differences were observed at blastocyst stage. During time-lapse monitoring, no difference was observed in the kinetics of 2-cell stage embryos. Compact morula (CM) stage was reached 15 h later in TSA treated embryos compared to the control. Blastocysts (Day 5 and 6) from HMC embryos treated with TSA were transferred to 2 recipients resulting in one pregnancy and birth of one live and five dead piglets. Our data demonstrate that TSA treatment after HMC in pigs may affect reprogramming of the somatic genome resulting in higher in vitro embryo development, and enable full-term in vivo development.

AB - Abnormal epigenetic modification is supposed to be one of factors accounting for inefficient reprogramming of the donor cell nuclei in ooplasm after somatic cell nuclear transfer (SCNT). Trichostatin A (TSA) is an inhibitor of histone deacetylase, potentially enhancing cloning efficiency. The aim of our present study was to establish the optimal TSA treatment in order to improve the development of handmade cloned (HMC) porcine embryos and examine the effect of TSA on their development. The blastocyst percentage of HMC embryos treated with 37.5 nM TSA for 22-24 h after activation increased up to 80% (control grpup-54%; P<0.05). TSA mediated increase in histone acetylation was proved by immunofluorescence analysis of acH3K9 and acH4K16. 2-cell stage embryos derived from TSA treatment displayed significant increase in histone acetylation compared to control embryos, whereas no significant differences were observed at blastocyst stage. During time-lapse monitoring, no difference was observed in the kinetics of 2-cell stage embryos. Compact morula (CM) stage was reached 15 h later in TSA treated embryos compared to the control. Blastocysts (Day 5 and 6) from HMC embryos treated with TSA were transferred to 2 recipients resulting in one pregnancy and birth of one live and five dead piglets. Our data demonstrate that TSA treatment after HMC in pigs may affect reprogramming of the somatic genome resulting in higher in vitro embryo development, and enable full-term in vivo development.

KW - Former LIFE faculty

KW - Pig

KW - Handmade cloning

KW - Histone acetylation

KW - Time-lapse

KW - Reprogramming

U2 - 10.1016/j.theriogenology.2008.05.046

DO - 10.1016/j.theriogenology.2008.05.046

M3 - Journal article

VL - 70

SP - 800

EP - 808

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

IS - 5

ER -

ID: 8109737