Identification of immunogenic and virulence-associated Campylobacter jejuni proteins

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Identification of immunogenic and virulence-associated Campylobacter jejuni proteins. / Nielsen, Lene Nørby; Luijkx, Thomas A.; Vegge, Christina Skovgaard; Johnsen, Christina Kofoed; Nuijten, Piet; Wren, Brendan W.; Ingmer, Hanne; Krogfelt, Karen Angeliki.

I: Clinical and Vaccine Immunology (Online), Bind 19, Nr. 2, 2012, s. 113-119.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nielsen, LN, Luijkx, TA, Vegge, CS, Johnsen, CK, Nuijten, P, Wren, BW, Ingmer, H & Krogfelt, KA 2012, 'Identification of immunogenic and virulence-associated Campylobacter jejuni proteins', Clinical and Vaccine Immunology (Online), bind 19, nr. 2, s. 113-119. https://doi.org/10.1128/CVI.05161-11

APA

Nielsen, L. N., Luijkx, T. A., Vegge, C. S., Johnsen, C. K., Nuijten, P., Wren, B. W., Ingmer, H., & Krogfelt, K. A. (2012). Identification of immunogenic and virulence-associated Campylobacter jejuni proteins. Clinical and Vaccine Immunology (Online), 19(2), 113-119. https://doi.org/10.1128/CVI.05161-11

Vancouver

Nielsen LN, Luijkx TA, Vegge CS, Johnsen CK, Nuijten P, Wren BW o.a. Identification of immunogenic and virulence-associated Campylobacter jejuni proteins. Clinical and Vaccine Immunology (Online). 2012;19(2):113-119. https://doi.org/10.1128/CVI.05161-11

Author

Nielsen, Lene Nørby ; Luijkx, Thomas A. ; Vegge, Christina Skovgaard ; Johnsen, Christina Kofoed ; Nuijten, Piet ; Wren, Brendan W. ; Ingmer, Hanne ; Krogfelt, Karen Angeliki. / Identification of immunogenic and virulence-associated Campylobacter jejuni proteins. I: Clinical and Vaccine Immunology (Online). 2012 ; Bind 19, Nr. 2. s. 113-119.

Bibtex

@article{142d7557658e476ea40abd763db97dd0,
title = "Identification of immunogenic and virulence-associated Campylobacter jejuni proteins",
abstract = "With the aim of identifying proteins important for host interaction and virulence, we have screened an expression library of NCTC 11168 Campylobacter jejuni genes for highly immunogenic proteins. A commercial C. jejuni open reading frame (ORF) library consisting of more than 1,600 genes was transformed into the Escherichia coli expression strain BL21(DE3), resulting in 2,304 clones. This library was subsequently screened for immunogenic proteins using antibodies raised in rabbit against a clinical isolate of C. jejuni; this resulted in 52 highly reactive clones representing 25 different genes after sequencing. Selected candidate genes were inactivated in C. jejuni NCTC 11168, and the virulence was examined using INT 407 epithelial cell line and motility, biofilm, autoagglutination, and serum resistance assays. These investigations revealed C. jejuni antigen 0034c (Cj0034c) to be a novel virulence factor and support the usefulness of the method. Further, several antigens were tested as vaccine candidates in two mouse models, in which Cj0034c, Cj0404, and Cj0525c resulted in a reduction of invasion in spleen and liver after challenge.",
author = "Nielsen, {Lene N{\o}rby} and Luijkx, {Thomas A.} and Vegge, {Christina Skovgaard} and Johnsen, {Christina Kofoed} and Piet Nuijten and Wren, {Brendan W.} and Hanne Ingmer and Krogfelt, {Karen Angeliki}",
year = "2012",
doi = "10.1128/CVI.05161-11",
language = "English",
volume = "19",
pages = "113--119",
journal = "Clinical and Vaccine Immunology (Online)",
issn = "1556-679X",
publisher = "American Society for Microbiology",
number = "2",

}

RIS

TY - JOUR

T1 - Identification of immunogenic and virulence-associated Campylobacter jejuni proteins

AU - Nielsen, Lene Nørby

AU - Luijkx, Thomas A.

AU - Vegge, Christina Skovgaard

AU - Johnsen, Christina Kofoed

AU - Nuijten, Piet

AU - Wren, Brendan W.

AU - Ingmer, Hanne

AU - Krogfelt, Karen Angeliki

PY - 2012

Y1 - 2012

N2 - With the aim of identifying proteins important for host interaction and virulence, we have screened an expression library of NCTC 11168 Campylobacter jejuni genes for highly immunogenic proteins. A commercial C. jejuni open reading frame (ORF) library consisting of more than 1,600 genes was transformed into the Escherichia coli expression strain BL21(DE3), resulting in 2,304 clones. This library was subsequently screened for immunogenic proteins using antibodies raised in rabbit against a clinical isolate of C. jejuni; this resulted in 52 highly reactive clones representing 25 different genes after sequencing. Selected candidate genes were inactivated in C. jejuni NCTC 11168, and the virulence was examined using INT 407 epithelial cell line and motility, biofilm, autoagglutination, and serum resistance assays. These investigations revealed C. jejuni antigen 0034c (Cj0034c) to be a novel virulence factor and support the usefulness of the method. Further, several antigens were tested as vaccine candidates in two mouse models, in which Cj0034c, Cj0404, and Cj0525c resulted in a reduction of invasion in spleen and liver after challenge.

AB - With the aim of identifying proteins important for host interaction and virulence, we have screened an expression library of NCTC 11168 Campylobacter jejuni genes for highly immunogenic proteins. A commercial C. jejuni open reading frame (ORF) library consisting of more than 1,600 genes was transformed into the Escherichia coli expression strain BL21(DE3), resulting in 2,304 clones. This library was subsequently screened for immunogenic proteins using antibodies raised in rabbit against a clinical isolate of C. jejuni; this resulted in 52 highly reactive clones representing 25 different genes after sequencing. Selected candidate genes were inactivated in C. jejuni NCTC 11168, and the virulence was examined using INT 407 epithelial cell line and motility, biofilm, autoagglutination, and serum resistance assays. These investigations revealed C. jejuni antigen 0034c (Cj0034c) to be a novel virulence factor and support the usefulness of the method. Further, several antigens were tested as vaccine candidates in two mouse models, in which Cj0034c, Cj0404, and Cj0525c resulted in a reduction of invasion in spleen and liver after challenge.

U2 - 10.1128/CVI.05161-11

DO - 10.1128/CVI.05161-11

M3 - Journal article

C2 - 22155767

VL - 19

SP - 113

EP - 119

JO - Clinical and Vaccine Immunology (Online)

JF - Clinical and Vaccine Immunology (Online)

SN - 1556-679X

IS - 2

ER -

ID: 37536877