Immunological and molecular techniques used for determination of serotypes in Pasteurellaceae
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Immunological and molecular techniques used for determination of serotypes in Pasteurellaceae. / Christensen, Henrik; Bossé, Janine; Angen, Øystein; Nørskov-Lauritsen, Niels; Bisgaard, Magne.
Methods in Microbiology. Bind 47 Academic Press, 2020. s. 117-149 (Methods in Microbiology).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - Immunological and molecular techniques used for determination of serotypes in Pasteurellaceae
AU - Christensen, Henrik
AU - Bossé, Janine
AU - Angen, Øystein
AU - Nørskov-Lauritsen, Niels
AU - Bisgaard, Magne
PY - 2020
Y1 - 2020
N2 - Serotyping has been used to categorize most pathogenic members of Pasteurellaceae of human or animal importance. In particular, serotyping of isolates from vaccine breaks is of primary importance to ensure that vaccines used are covering outbreak isolates in the region. Traditional serotyping methods are available for Pasteurella multocida, Actinobacillus pleuropneumoniae, Glaesserella parasuis (older name Haemophilus parasuis), Mannheimia haemolytica, Avibacterium paragallinarum, Haemophilus influenzae, and Aggregatibacter actinomycetemcomitans. PCR-based methods to predict the serotypes have been developed for all agents with the exception of Avibacterium paragallinarum. For several of the species, work is in progress to predict serotypes from whole genomic sequences. The combination of population structures determined by whole genomic sequencing and the prediction of serotypes provides a strong synergy in epidemiological investigations of the species of Pasteurellaceae involved in disease in humans and animals.
AB - Serotyping has been used to categorize most pathogenic members of Pasteurellaceae of human or animal importance. In particular, serotyping of isolates from vaccine breaks is of primary importance to ensure that vaccines used are covering outbreak isolates in the region. Traditional serotyping methods are available for Pasteurella multocida, Actinobacillus pleuropneumoniae, Glaesserella parasuis (older name Haemophilus parasuis), Mannheimia haemolytica, Avibacterium paragallinarum, Haemophilus influenzae, and Aggregatibacter actinomycetemcomitans. PCR-based methods to predict the serotypes have been developed for all agents with the exception of Avibacterium paragallinarum. For several of the species, work is in progress to predict serotypes from whole genomic sequences. The combination of population structures determined by whole genomic sequencing and the prediction of serotypes provides a strong synergy in epidemiological investigations of the species of Pasteurellaceae involved in disease in humans and animals.
KW - Identification
KW - Indirect haemagglutination
KW - PCR
KW - Serology
KW - Serovar
U2 - 10.1016/bs.mim.2020.01.002
DO - 10.1016/bs.mim.2020.01.002
M3 - Book chapter
AN - SCOPUS:85083322475
VL - 47
T3 - Methods in Microbiology
SP - 117
EP - 149
BT - Methods in Microbiology
PB - Academic Press
ER -
ID: 240149239