Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells

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Standard

Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells. / Koch, Thomas Gadegaard; Thomsen, Preben Dybdahl; Betts, Dean H.

I: Cytotherapy, Bind 11, Nr. 4, 2009, s. 443-447.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Koch, TG, Thomsen, PD & Betts, DH 2009, 'Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells', Cytotherapy, bind 11, nr. 4, s. 443-447. https://doi.org/10.1080/14653240902887259

APA

Koch, T. G., Thomsen, P. D., & Betts, D. H. (2009). Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells. Cytotherapy, 11(4), 443-447. https://doi.org/10.1080/14653240902887259

Vancouver

Koch TG, Thomsen PD, Betts DH. Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells. Cytotherapy. 2009;11(4):443-447. https://doi.org/10.1080/14653240902887259

Author

Koch, Thomas Gadegaard ; Thomsen, Preben Dybdahl ; Betts, Dean H. / Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells. I: Cytotherapy. 2009 ; Bind 11, Nr. 4. s. 443-447.

Bibtex

@article{2c6a3e309d2311debc73000ea68e967b,
title = "Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells",
abstract = "  BACKGROUND AIMS: A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. METHODS: Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. RESULTS AND CONCLUSIONS: Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.",
author = "Koch, {Thomas Gadegaard} and Thomsen, {Preben Dybdahl} and Betts, {Dean H.}",
year = "2009",
doi = "10.1080/14653240902887259",
language = "English",
volume = "11",
pages = "443--447",
journal = "Cytotherapy",
issn = "1465-3249",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells

AU - Koch, Thomas Gadegaard

AU - Thomsen, Preben Dybdahl

AU - Betts, Dean H.

PY - 2009

Y1 - 2009

N2 -   BACKGROUND AIMS: A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. METHODS: Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. RESULTS AND CONCLUSIONS: Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.

AB -   BACKGROUND AIMS: A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. METHODS: Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. RESULTS AND CONCLUSIONS: Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.

U2 - 10.1080/14653240902887259

DO - 10.1080/14653240902887259

M3 - Journal article

C2 - 19513899

VL - 11

SP - 443

EP - 447

JO - Cytotherapy

JF - Cytotherapy

SN - 1465-3249

IS - 4

ER -

ID: 14303884