Incidence, diversity, and molecular epidemiology of sapoviruses in swine across Europe
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Incidence, diversity, and molecular epidemiology of sapoviruses in swine across Europe. / Reuter, Gábor; Zimšek-Mijovski, Janet; Poljšak-Prijatelj, Mateja; Di Bartolo, Ilaria; Ruggeri, Franco Maria; Kantala, Tuija; Maunula, Leena; Kiss, István; Kecskeméti, Sándor; Halaihel, Nabil; Buesa, Javier; Johnsen, Christina; Hjulsager, Charlotte K.; Larsen, Lars E.; Koopmans, Marion; Blenda Böttiger, Á.
I: Journal of clinical microbiology, Bind 48, Nr. 2, 02.2010, s. 363-368.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Incidence, diversity, and molecular epidemiology of sapoviruses in swine across Europe
AU - Reuter, Gábor
AU - Zimšek-Mijovski, Janet
AU - Poljšak-Prijatelj, Mateja
AU - Di Bartolo, Ilaria
AU - Ruggeri, Franco Maria
AU - Kantala, Tuija
AU - Maunula, Leena
AU - Kiss, István
AU - Kecskeméti, Sándor
AU - Halaihel, Nabil
AU - Buesa, Javier
AU - Johnsen, Christina
AU - Hjulsager, Charlotte K.
AU - Larsen, Lars E.
AU - Koopmans, Marion
AU - Blenda Böttiger, Á
PY - 2010/2
Y1 - 2010/2
N2 - Porcine sapovirus is an enteric calicivirus in domestic pigs that belongs to the family Caliciviridae. Some porcine sapoviruses are genetically related to human caliciviruses, which has raised public health concerns over animal reservoirs and the potential cross-species transmission of sapoviruses. We report on the incidence, genetic diversity, and molecular epidemiology of sapoviruses detected in domestic pigs in a comprehensive study conducted in six European countries (Denmark, Finland, Hungary, Italy, Slovenia, and Spain) between 2004 and 2007. A total of 1,050 swine fecal samples from 88 pig farms were collected and tested by reverse transcription-PCR for sapoviruses, and positive findings were confirmed by sequencing. Sapoviruses were detected in 80 (7.6%) samples collected on 39 (44.3%) farms and in every country. The highest prevalence was seen among piglets aged 2 to 8 weeks, and there was no significant difference in the proportion of sapovirus-positive findings for healthy animals and animals with diarrhea in Spain and Denmark (the only countries where both healthy animals and animals with diarrhea were tested). On the basis of the sequence of the RNA polymerase region, highly heterogeneous populations of viruses representing six different genogroups (genogroups III, VI, VII, and VIII, including potential new genogroups IX and X) were identified, with a predominance of genogroup GIII (50.6%). Genogroup VIII, found in five of the six countries, had the highest degree of homology (up to 66% at the amino acid level) to human sapovirus strains. Sapoviruses are commonly circulating and endemic agents in swine herds throughout Europe. Highly heterogeneous and potential new genogroups of sapoviruses were found in pigs; however, no "human-like" sapoviruses were detected.
AB - Porcine sapovirus is an enteric calicivirus in domestic pigs that belongs to the family Caliciviridae. Some porcine sapoviruses are genetically related to human caliciviruses, which has raised public health concerns over animal reservoirs and the potential cross-species transmission of sapoviruses. We report on the incidence, genetic diversity, and molecular epidemiology of sapoviruses detected in domestic pigs in a comprehensive study conducted in six European countries (Denmark, Finland, Hungary, Italy, Slovenia, and Spain) between 2004 and 2007. A total of 1,050 swine fecal samples from 88 pig farms were collected and tested by reverse transcription-PCR for sapoviruses, and positive findings were confirmed by sequencing. Sapoviruses were detected in 80 (7.6%) samples collected on 39 (44.3%) farms and in every country. The highest prevalence was seen among piglets aged 2 to 8 weeks, and there was no significant difference in the proportion of sapovirus-positive findings for healthy animals and animals with diarrhea in Spain and Denmark (the only countries where both healthy animals and animals with diarrhea were tested). On the basis of the sequence of the RNA polymerase region, highly heterogeneous populations of viruses representing six different genogroups (genogroups III, VI, VII, and VIII, including potential new genogroups IX and X) were identified, with a predominance of genogroup GIII (50.6%). Genogroup VIII, found in five of the six countries, had the highest degree of homology (up to 66% at the amino acid level) to human sapovirus strains. Sapoviruses are commonly circulating and endemic agents in swine herds throughout Europe. Highly heterogeneous and potential new genogroups of sapoviruses were found in pigs; however, no "human-like" sapoviruses were detected.
UR - http://www.scopus.com/inward/record.url?scp=75649133890&partnerID=8YFLogxK
U2 - 10.1128/JCM.01279-09
DO - 10.1128/JCM.01279-09
M3 - Journal article
C2 - 19940055
AN - SCOPUS:75649133890
VL - 48
SP - 363
EP - 368
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
SN - 0095-1137
IS - 2
ER -
ID: 247399090