Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets

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Standard

Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets. / Pedersen, Lotte Gam; Zhao, J.; Yang, J.; Thomsen, Preben Dybdahl; Gregersen, H.; Hasenkam, J.M.; Smerup, M.; Pedersen, H.D.; Olsen, Lisbeth Høier.

I: Research in Veterinary Science, Bind 82, Nr. 2, 2007, s. 232-238.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Pedersen, LG, Zhao, J, Yang, J, Thomsen, PD, Gregersen, H, Hasenkam, JM, Smerup, M, Pedersen, HD & Olsen, LH 2007, 'Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets', Research in Veterinary Science, bind 82, nr. 2, s. 232-238. https://doi.org/10.1016/j.rvsc.2006.07.009

APA

Pedersen, L. G., Zhao, J., Yang, J., Thomsen, P. D., Gregersen, H., Hasenkam, J. M., Smerup, M., Pedersen, H. D., & Olsen, L. H. (2007). Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets. Research in Veterinary Science, 82(2), 232-238. https://doi.org/10.1016/j.rvsc.2006.07.009

Vancouver

Pedersen LG, Zhao J, Yang J, Thomsen PD, Gregersen H, Hasenkam JM o.a. Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets. Research in Veterinary Science. 2007;82(2):232-238. https://doi.org/10.1016/j.rvsc.2006.07.009

Author

Pedersen, Lotte Gam ; Zhao, J. ; Yang, J. ; Thomsen, Preben Dybdahl ; Gregersen, H. ; Hasenkam, J.M. ; Smerup, M. ; Pedersen, H.D. ; Olsen, Lisbeth Høier. / Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets. I: Research in Veterinary Science. 2007 ; Bind 82, Nr. 2. s. 232-238.

Bibtex

@article{6eb09810a1c111ddb6ae000ea68e967b,
title = "Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets",
abstract = "The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.",
keywords = "Former LIFE faculty, Endothelin, Endothelin receptors, Mitral valve, Real-time PCR, Stress, Stretch",
author = "Pedersen, {Lotte Gam} and J. Zhao and J. Yang and Thomsen, {Preben Dybdahl} and H. Gregersen and J.M. Hasenkam and M. Smerup and H.D. Pedersen and Olsen, {Lisbeth H{\o}ier}",
year = "2007",
doi = "10.1016/j.rvsc.2006.07.009",
language = "English",
volume = "82",
pages = "232--238",
journal = "Research in Veterinary Science",
issn = "0034-5288",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets

AU - Pedersen, Lotte Gam

AU - Zhao, J.

AU - Yang, J.

AU - Thomsen, Preben Dybdahl

AU - Gregersen, H.

AU - Hasenkam, J.M.

AU - Smerup, M.

AU - Pedersen, H.D.

AU - Olsen, Lisbeth Høier

PY - 2007

Y1 - 2007

N2 - The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.

AB - The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37oC together with matching control segments. Subsequently, the mRNA expression of ET-1, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease.

KW - Former LIFE faculty

KW - Endothelin

KW - Endothelin receptors

KW - Mitral valve

KW - Real-time PCR

KW - Stress

KW - Stretch

U2 - 10.1016/j.rvsc.2006.07.009

DO - 10.1016/j.rvsc.2006.07.009

M3 - Journal article

C2 - 17011002

VL - 82

SP - 232

EP - 238

JO - Research in Veterinary Science

JF - Research in Veterinary Science

SN - 0034-5288

IS - 2

ER -

ID: 8045011