Inflammatory proteins in infected bone tissue – An explorative porcine study
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Inflammatory proteins in infected bone tissue – An explorative porcine study. / Bue, Mats; Bergholt, Natasja Leth; Jensen, Louise Kruse; Jensen, Henrik Elvang; Søballe, Kjeld; Stilling, Maiken; Hanberg, Pelle.
I: Bone Reports, Bind 13, 100292, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Inflammatory proteins in infected bone tissue – An explorative porcine study
AU - Bue, Mats
AU - Bergholt, Natasja Leth
AU - Jensen, Louise Kruse
AU - Jensen, Henrik Elvang
AU - Søballe, Kjeld
AU - Stilling, Maiken
AU - Hanberg, Pelle
PY - 2020
Y1 - 2020
N2 - Objective: To explore the in situ inflammatory proteins in the local extracellular fluid of infected bone tissue. Material and methods: Seven pigs went through a two-step surgery performing a traumatically implant-associated Staphylococcus aureus osteomyelitis in the proximal tibia. Five days later, microdialysis catheters (membrane cut off: 20 kDa) were placed in the implant cavity, infected and healthy cancellous bone, and infected and healthy subcutaneous tissue. Plasma samples were collected simultaneously. We employed an antibody-based proximity extension assay (Olink Inflammatory panel) for the measurement of inflammatory molecules within plasma and extracellular fluid of the investigated tissue compartments. Results: A higher normalized protein expression in the infected bone tissue in comparison to healthy bone tissue was identified for proteins associated with angiogenesis and bone remodeling: OPG, TGFα, MCP-1, VEGFA, and uPA. Moreover, a parallel detectability of the systemic range of cytokines and chemokines as from the investigated local tissue compartments was demonstrated, indicating the same occurrence of proteins in the local environment as within plasma. Conclusion: An angiogenic and osteogenic inflammatory protein composition within the extracellular fluid of infected bone tissue was described. The findings support the current histopathological knowledge and, therefore, microdialysis may represent a valid method for sampling of material for protein investigation of the in vivo inflammatory composition within the extracellular environment in infected bone tissue.
AB - Objective: To explore the in situ inflammatory proteins in the local extracellular fluid of infected bone tissue. Material and methods: Seven pigs went through a two-step surgery performing a traumatically implant-associated Staphylococcus aureus osteomyelitis in the proximal tibia. Five days later, microdialysis catheters (membrane cut off: 20 kDa) were placed in the implant cavity, infected and healthy cancellous bone, and infected and healthy subcutaneous tissue. Plasma samples were collected simultaneously. We employed an antibody-based proximity extension assay (Olink Inflammatory panel) for the measurement of inflammatory molecules within plasma and extracellular fluid of the investigated tissue compartments. Results: A higher normalized protein expression in the infected bone tissue in comparison to healthy bone tissue was identified for proteins associated with angiogenesis and bone remodeling: OPG, TGFα, MCP-1, VEGFA, and uPA. Moreover, a parallel detectability of the systemic range of cytokines and chemokines as from the investigated local tissue compartments was demonstrated, indicating the same occurrence of proteins in the local environment as within plasma. Conclusion: An angiogenic and osteogenic inflammatory protein composition within the extracellular fluid of infected bone tissue was described. The findings support the current histopathological knowledge and, therefore, microdialysis may represent a valid method for sampling of material for protein investigation of the in vivo inflammatory composition within the extracellular environment in infected bone tissue.
KW - Fluid space of bone
KW - Inflammatory proteins in infected bone tissue
KW - Microdialysis
KW - Osteomyelitis porcine model
KW - Proximity extension assay
U2 - 10.1016/j.bonr.2020.100292
DO - 10.1016/j.bonr.2020.100292
M3 - Journal article
C2 - 32637468
AN - SCOPUS:85087032862
VL - 13
JO - Bone Reports
JF - Bone Reports
SN - 2352-1872
M1 - 100292
ER -
ID: 244612605