TY - JOUR

T1 - Interbreed variation in serum serotonin (5-hydroxytryptamine) concentration in updates healthy dogs

AU - Hoglund, K.

AU - Haggstrom, J.

AU - Hanås, S.

AU - Merveille, A. -C.

AU - Gouni, V.

AU - Wiberg, M.

AU - Willesen, J. Lundgren

AU - Mc Entee, K.

AU - Sorensen, L. Mejer

AU - Tiret, L.

AU - Seppala, E. H.

AU - Lohi, H.

AU - Chetboul, V.

AU - Fredholm, M.

AU - Lequarre, A. -S.

AU - Ljungvall, I.

PY - 2018

Y1 - 2018

N2 - ntroduction Serotonin (5-hydroxytryptamine [5-HT]) has several biological functions. In different species, excessive 5-HT has been linked to valvular lesions, similar to those seen in dogs with myxomatous mitral valve disease. Previous studies suggest higher 5-HT in healthy Cavalier King Charles Spaniels (CKCSs), a breed highly affected by myxomatous mitral valve disease, compared to other breeds. Objective To investigate potential interbreed variation in serum 5-HT in healthy dogs. Animals 483 healthy dogs of nine breeds aged 1–7 years. Methods Dogs were examined at five European centers. Absence of cardiovascular, organ-related, or systemic diseases was ensured by thorough clinical investigations including echocardiography. Serum was frozen and later analyzed by enzyme-linked immunosorbent assay (ELISA). Results Median 5-HT concentration was 252.5 (interquartile range = 145.5–390.6) ng/mL. Overall breed difference was found (p<0.0001), and 42% of pairwise breed comparisons were significant. Univariate regression analysis showed association between serum 5-HT concentration and breed, center of examination, storage time, and sex, with higher 5-HT in females. In multiple regression analysis, the final model had an adjusted R2 of 0.27 with breed (p<0.0001), center (p<0.0001), and storage time (p=0.014) remaining significant. Within centers, overall breed differences were found at 3/5 centers (p≤0.028), and pairwise comparisons within those centers showed breed differences in 42% of comparisons. Among the included breeds, Newfoundlands, Belgian Shepherds and CKCSs had highest 5-HT concentrations. Conclusions Interbreed variation in serum 5-HT concentration was found in healthy dogs aged 1–7 years. These differences should be taken into account when designing clinical studies.

AB - ntroduction Serotonin (5-hydroxytryptamine [5-HT]) has several biological functions. In different species, excessive 5-HT has been linked to valvular lesions, similar to those seen in dogs with myxomatous mitral valve disease. Previous studies suggest higher 5-HT in healthy Cavalier King Charles Spaniels (CKCSs), a breed highly affected by myxomatous mitral valve disease, compared to other breeds. Objective To investigate potential interbreed variation in serum 5-HT in healthy dogs. Animals 483 healthy dogs of nine breeds aged 1–7 years. Methods Dogs were examined at five European centers. Absence of cardiovascular, organ-related, or systemic diseases was ensured by thorough clinical investigations including echocardiography. Serum was frozen and later analyzed by enzyme-linked immunosorbent assay (ELISA). Results Median 5-HT concentration was 252.5 (interquartile range = 145.5–390.6) ng/mL. Overall breed difference was found (p<0.0001), and 42% of pairwise breed comparisons were significant. Univariate regression analysis showed association between serum 5-HT concentration and breed, center of examination, storage time, and sex, with higher 5-HT in females. In multiple regression analysis, the final model had an adjusted R2 of 0.27 with breed (p<0.0001), center (p<0.0001), and storage time (p=0.014) remaining significant. Within centers, overall breed differences were found at 3/5 centers (p≤0.028), and pairwise comparisons within those centers showed breed differences in 42% of comparisons. Among the included breeds, Newfoundlands, Belgian Shepherds and CKCSs had highest 5-HT concentrations. Conclusions Interbreed variation in serum 5-HT concentration was found in healthy dogs aged 1–7 years. These differences should be taken into account when designing clinical studies.

KW - Breed difference

KW - Biomarker

KW - Canine

KW - Heart valve

KW - 5-HT

U2 - 10.1016/j.jvc.2018.05.002

DO - 10.1016/j.jvc.2018.05.002

M3 - Journal article

C2 - 29921518

VL - 20

SP - 244

EP - 253

JO - Journal of Veterinary Cardiology

JF - Journal of Veterinary Cardiology

SN - 1760-2734

IS - 4

ER -