TY - ABST

T1 - Investigations of FIBCD1

T2 - Immunohistochemical localization and immunomodulatory role upon helminth antigen stimulation in colon epithelium

AU - von Huth, Sebastian

AU - Skallerup, Sofie

AU - Buragaite, Benita

AU - Schlosser, Anders

AU - Moeller, Jesper B.

AU - Hammond, Mark

AU - Nielsen, Ole

AU - Marcussen, Niels

AU - Svensson-Frej, Marcus

AU - Williams, Andrew R.

AU - Thamsborg, Stig M.

AU - Sorensen, Grith L.

AU - Holmskov, Uffe

PY - 2017

Y1 - 2017

N2 - IntroductionFibrinogen C domain-containing 1 (FIBCD1) is a homotetrameric type II transmembrane protein, expressed at epithelial surfaces. FIBCD1 has a wide ligand spectrum, including chitin found in various pathogens such as helminths and fungi. Current understanding points towards a role as a pattern recognition receptor in the innate immune response.AimIn the present study, we investigate the localization of FIBCD1 in 49 different healthy human tissues by immunohistochemistry. Further, we investigate the in vitro effects of excretory-secretory (ES) antigens from pig whipworm (Trichuris suis) on FIBCD1-transfected colon epithelial cells, and identify protein fractions in the ES-antigen that binds to FIBCD1.ResultsImmunohistochemical staining shows that FIBCD1 is present at mucosal surfaces throughout the human body, with high intensity in the airways and gastrointestinal and urogenital tract. Transfection of the human colon epithelial cell line HCT116 with FIBCD1 did not result in any major genotypic differences upon stimulation with ES antigens, as determined by quantitative PCR of various pro- and anti-inflammatory genes. FIBCD1 recognizes and binds to whipworm eggshell and ES antigens. Analysis of the T. suis ES-antigen by size chromatography reveals predominant sizes of proteins ranging from 10 kDa to 200 kDa.ConclusionFIBCD1 is present at mucosal surfaces throughout the human body, which may underline its function as a pattern recognition receptor in the innate immune system. FIBCD1 binds to ES antigens. Stimulation with ES antigens does not appear to be influenced by FIBCD1 transfection in vitro. FIBCD1 may play a role in whipworm infections in vivo.

AB - IntroductionFibrinogen C domain-containing 1 (FIBCD1) is a homotetrameric type II transmembrane protein, expressed at epithelial surfaces. FIBCD1 has a wide ligand spectrum, including chitin found in various pathogens such as helminths and fungi. Current understanding points towards a role as a pattern recognition receptor in the innate immune response.AimIn the present study, we investigate the localization of FIBCD1 in 49 different healthy human tissues by immunohistochemistry. Further, we investigate the in vitro effects of excretory-secretory (ES) antigens from pig whipworm (Trichuris suis) on FIBCD1-transfected colon epithelial cells, and identify protein fractions in the ES-antigen that binds to FIBCD1.ResultsImmunohistochemical staining shows that FIBCD1 is present at mucosal surfaces throughout the human body, with high intensity in the airways and gastrointestinal and urogenital tract. Transfection of the human colon epithelial cell line HCT116 with FIBCD1 did not result in any major genotypic differences upon stimulation with ES antigens, as determined by quantitative PCR of various pro- and anti-inflammatory genes. FIBCD1 recognizes and binds to whipworm eggshell and ES antigens. Analysis of the T. suis ES-antigen by size chromatography reveals predominant sizes of proteins ranging from 10 kDa to 200 kDa.ConclusionFIBCD1 is present at mucosal surfaces throughout the human body, which may underline its function as a pattern recognition receptor in the innate immune system. FIBCD1 binds to ES antigens. Stimulation with ES antigens does not appear to be influenced by FIBCD1 transfection in vitro. FIBCD1 may play a role in whipworm infections in vivo.

U2 - 10.1111/sji.12587

DO - 10.1111/sji.12587

M3 - Conference abstract in journal

VL - 86

SP - 255

EP - 255

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

SN - 0301-6323

IS - 4

M1 - A-31195

ER -