Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress

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Standard

Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress. / Shabala, Lana; McMeekin, Tom; Budde, Birgitte Bjørn; Siegumfeldt, Henrik.

I: International Journal of Food Microbiology, Bind 110, Nr. 1, 2006, s. 1-7.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Shabala, L, McMeekin, T, Budde, BB & Siegumfeldt, H 2006, 'Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress', International Journal of Food Microbiology, bind 110, nr. 1, s. 1-7. https://doi.org/10.1016/j.ijfoodmicro.2006.01.026

APA

Shabala, L., McMeekin, T., Budde, B. B., & Siegumfeldt, H. (2006). Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress. International Journal of Food Microbiology, 110(1), 1-7. https://doi.org/10.1016/j.ijfoodmicro.2006.01.026

Vancouver

Shabala L, McMeekin T, Budde BB, Siegumfeldt H. Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress. International Journal of Food Microbiology. 2006;110(1):1-7. https://doi.org/10.1016/j.ijfoodmicro.2006.01.026

Author

Shabala, Lana ; McMeekin, Tom ; Budde, Birgitte Bjørn ; Siegumfeldt, Henrik. / Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress. I: International Journal of Food Microbiology. 2006 ; Bind 110, Nr. 1. s. 1-7.

Bibtex

@article{0cda06a0a1c411ddb6ae000ea68e967b,
title = "Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress",
abstract = "Responses of Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus to a rapid change in extracellular pH (pHex) from pHex 6 to a range of concentrations down to pHex 3.0 were examined, using HCl and lactic acid (LA) as acidulants. A new fluorescent probe 5-(and-6)-carboxy-2', 7'-dichlorofluorescein diacetate succinimidyl ester (CDCFDA-SE) was employed that enabled reliable measurements of intracellular pH (pHi) to a minimum pHi of 4.0. Changes in pHi and H+ fluxes from immobilised bacteria were measured using fluorescence ratio imaging microscopy (FRIM) and a non-invasive ion flux measuring technique (MIFE), respectively. L. innocua maintained a relatively constant pHi of 5.5-6.1 at pHex 4 and 5 via H+ extrusion. In contrast, L. delbrueckii subsp. bulgaricus progressively lowered pHi towards pHex over the entire pHex range examined. The type of acidulant used influenced pH regulation with both pHi and H+ -fluxes being more severely affected by LA compared to HCl. Overall, our data demonstrated different adaptive strategies in these two bacteria. While L. innocua expels protons to maintain a constant pHi, L. delbrueckii subsp. bulgaricus allows proton entry after acidic treatment so that pHi follows pHex.",
keywords = "Former LIFE faculty, Listeria innocua; Lactobacillus delbrueckii subsp. bulgaricus; Acid stress; pH homeostasis; pHi; H+ ion flux; Microelectrode technique",
author = "Lana Shabala and Tom McMeekin and Budde, {Birgitte Bj{\o}rn} and Henrik Siegumfeldt",
year = "2006",
doi = "10.1016/j.ijfoodmicro.2006.01.026",
language = "English",
volume = "110",
pages = "1--7",
journal = "International Journal of Food Microbiology",
issn = "0168-1605",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress

AU - Shabala, Lana

AU - McMeekin, Tom

AU - Budde, Birgitte Bjørn

AU - Siegumfeldt, Henrik

PY - 2006

Y1 - 2006

N2 - Responses of Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus to a rapid change in extracellular pH (pHex) from pHex 6 to a range of concentrations down to pHex 3.0 were examined, using HCl and lactic acid (LA) as acidulants. A new fluorescent probe 5-(and-6)-carboxy-2', 7'-dichlorofluorescein diacetate succinimidyl ester (CDCFDA-SE) was employed that enabled reliable measurements of intracellular pH (pHi) to a minimum pHi of 4.0. Changes in pHi and H+ fluxes from immobilised bacteria were measured using fluorescence ratio imaging microscopy (FRIM) and a non-invasive ion flux measuring technique (MIFE), respectively. L. innocua maintained a relatively constant pHi of 5.5-6.1 at pHex 4 and 5 via H+ extrusion. In contrast, L. delbrueckii subsp. bulgaricus progressively lowered pHi towards pHex over the entire pHex range examined. The type of acidulant used influenced pH regulation with both pHi and H+ -fluxes being more severely affected by LA compared to HCl. Overall, our data demonstrated different adaptive strategies in these two bacteria. While L. innocua expels protons to maintain a constant pHi, L. delbrueckii subsp. bulgaricus allows proton entry after acidic treatment so that pHi follows pHex.

AB - Responses of Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus to a rapid change in extracellular pH (pHex) from pHex 6 to a range of concentrations down to pHex 3.0 were examined, using HCl and lactic acid (LA) as acidulants. A new fluorescent probe 5-(and-6)-carboxy-2', 7'-dichlorofluorescein diacetate succinimidyl ester (CDCFDA-SE) was employed that enabled reliable measurements of intracellular pH (pHi) to a minimum pHi of 4.0. Changes in pHi and H+ fluxes from immobilised bacteria were measured using fluorescence ratio imaging microscopy (FRIM) and a non-invasive ion flux measuring technique (MIFE), respectively. L. innocua maintained a relatively constant pHi of 5.5-6.1 at pHex 4 and 5 via H+ extrusion. In contrast, L. delbrueckii subsp. bulgaricus progressively lowered pHi towards pHex over the entire pHex range examined. The type of acidulant used influenced pH regulation with both pHi and H+ -fluxes being more severely affected by LA compared to HCl. Overall, our data demonstrated different adaptive strategies in these two bacteria. While L. innocua expels protons to maintain a constant pHi, L. delbrueckii subsp. bulgaricus allows proton entry after acidic treatment so that pHi follows pHex.

KW - Former LIFE faculty

KW - Listeria innocua; Lactobacillus delbrueckii subsp. bulgaricus; Acid stress; pH homeostasis; pHi; H+ ion flux; Microelectrode technique

U2 - 10.1016/j.ijfoodmicro.2006.01.026

DO - 10.1016/j.ijfoodmicro.2006.01.026

M3 - Journal article

C2 - 16716423

VL - 110

SP - 1

EP - 7

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

IS - 1

ER -

ID: 8108410