Localization of thymosin ß10 in breast cancer cells: relationship to actin cytoskeletal remodeling and cell motility

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Standard

Localization of thymosin ß10 in breast cancer cells : relationship to actin cytoskeletal remodeling and cell motility. / Mælan, A.ase Elisabeth; Rasmussen, Trine Kring; Larsson, Lars-Inge.

I: Histochemistry and Cell Biology, Bind 127, Nr. 1, 2007, s. 109-113.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Mælan, AAE, Rasmussen, TK & Larsson, L-I 2007, 'Localization of thymosin ß10 in breast cancer cells: relationship to actin cytoskeletal remodeling and cell motility', Histochemistry and Cell Biology, bind 127, nr. 1, s. 109-113. https://doi.org/10.1007/s00418-006-0208-z

APA

Mælan, A. A. E., Rasmussen, T. K., & Larsson, L-I. (2007). Localization of thymosin ß10 in breast cancer cells: relationship to actin cytoskeletal remodeling and cell motility. Histochemistry and Cell Biology, 127(1), 109-113. https://doi.org/10.1007/s00418-006-0208-z

Vancouver

Mælan AAE, Rasmussen TK, Larsson L-I. Localization of thymosin ß10 in breast cancer cells: relationship to actin cytoskeletal remodeling and cell motility. Histochemistry and Cell Biology. 2007;127(1):109-113. https://doi.org/10.1007/s00418-006-0208-z

Author

Mælan, A.ase Elisabeth ; Rasmussen, Trine Kring ; Larsson, Lars-Inge. / Localization of thymosin ß10 in breast cancer cells : relationship to actin cytoskeletal remodeling and cell motility. I: Histochemistry and Cell Biology. 2007 ; Bind 127, Nr. 1. s. 109-113.

Bibtex

@article{71aa7720a1c111ddb6ae000ea68e967b,
title = "Localization of thymosin {\ss}10 in breast cancer cells: relationship to actin cytoskeletal remodeling and cell motility",
abstract = "Beta-thymosins are polypeptides involved in the regulation of actin polymerization and thymosin {\ss}10 and {\ss}4 have been implicated in sequestration of monomeric (G-) actin. Additionally, experimental overexpression of thymosin {\ss}10 has been found to result in increases in F-actin bundles as well as in cell motility and spreading. We have studied the distribution of endogenously expressed thymosin {\ss}10 in cultured human breast cancer cell lines. Both unperturbed monolayer cultures and wound-healing models were examined using double-staining for thymosin {\ss}10 and polymerized (F-) actin. Our findings show that thymosin {\ss}10 is expressed in all three-cancer cell lines (SK-BR-3, MCF-7 and MDA-MB-231) studied. No or little staining was detected in confluent cells, whereas strong staining occurred in semiconfluent cells and in cells populating monolayer wounds. Importantly, the distribution of staining for thymosin {\ss}10 was inverse of staining for F-actin. These data support a physiological role for thymosin {\ss}10 in sequestration of G-actin as well as in cancer cell motility.",
keywords = "Former LIFE faculty, Thymosin {\ss}10, Breast cancer, F-actin, G-actin, Motility, Cell culture, Immunofluorescence, Wound healing",
author = "M{\ae}lan, {A.ase Elisabeth} and Rasmussen, {Trine Kring} and Lars-Inge Larsson",
year = "2007",
doi = "10.1007/s00418-006-0208-z",
language = "English",
volume = "127",
pages = "109--113",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Localization of thymosin ß10 in breast cancer cells

T2 - relationship to actin cytoskeletal remodeling and cell motility

AU - Mælan, A.ase Elisabeth

AU - Rasmussen, Trine Kring

AU - Larsson, Lars-Inge

PY - 2007

Y1 - 2007

N2 - Beta-thymosins are polypeptides involved in the regulation of actin polymerization and thymosin ß10 and ß4 have been implicated in sequestration of monomeric (G-) actin. Additionally, experimental overexpression of thymosin ß10 has been found to result in increases in F-actin bundles as well as in cell motility and spreading. We have studied the distribution of endogenously expressed thymosin ß10 in cultured human breast cancer cell lines. Both unperturbed monolayer cultures and wound-healing models were examined using double-staining for thymosin ß10 and polymerized (F-) actin. Our findings show that thymosin ß10 is expressed in all three-cancer cell lines (SK-BR-3, MCF-7 and MDA-MB-231) studied. No or little staining was detected in confluent cells, whereas strong staining occurred in semiconfluent cells and in cells populating monolayer wounds. Importantly, the distribution of staining for thymosin ß10 was inverse of staining for F-actin. These data support a physiological role for thymosin ß10 in sequestration of G-actin as well as in cancer cell motility.

AB - Beta-thymosins are polypeptides involved in the regulation of actin polymerization and thymosin ß10 and ß4 have been implicated in sequestration of monomeric (G-) actin. Additionally, experimental overexpression of thymosin ß10 has been found to result in increases in F-actin bundles as well as in cell motility and spreading. We have studied the distribution of endogenously expressed thymosin ß10 in cultured human breast cancer cell lines. Both unperturbed monolayer cultures and wound-healing models were examined using double-staining for thymosin ß10 and polymerized (F-) actin. Our findings show that thymosin ß10 is expressed in all three-cancer cell lines (SK-BR-3, MCF-7 and MDA-MB-231) studied. No or little staining was detected in confluent cells, whereas strong staining occurred in semiconfluent cells and in cells populating monolayer wounds. Importantly, the distribution of staining for thymosin ß10 was inverse of staining for F-actin. These data support a physiological role for thymosin ß10 in sequestration of G-actin as well as in cancer cell motility.

KW - Former LIFE faculty

KW - Thymosin ß10

KW - Breast cancer

KW - F-actin

KW - G-actin

KW - Motility

KW - Cell culture

KW - Immunofluorescence

KW - Wound healing

U2 - 10.1007/s00418-006-0208-z

DO - 10.1007/s00418-006-0208-z

M3 - Journal article

C2 - 16786322

VL - 127

SP - 109

EP - 113

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 1

ER -

ID: 8045394