Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni
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Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni. / Gencay, Yilmaz Emre; Birk, Tina; Sørensen, Martine Camilla Holst; Brøndsted, Lone.
Campylobacter jejuni: Methods and Protocols. Bind 1512 Humana Press, 2017. s. 19-28 (Methods in Molecular Biology, Bind 1512).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni
AU - Gencay, Yilmaz Emre
AU - Birk, Tina
AU - Sørensen, Martine Camilla Holst
AU - Brøndsted, Lone
PY - 2017
Y1 - 2017
N2 - Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
AB - Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
KW - Bacteriophage
KW - C. jejuni
KW - Campylobacter
KW - Isolation
KW - Propagation
KW - Purification
KW - Titration
U2 - 10.1007/978-1-4939-6536-6_3
DO - 10.1007/978-1-4939-6536-6_3
M3 - Book chapter
C2 - 27885595
AN - SCOPUS:84997235896
SN - 978-1-4939-6534-2
VL - 1512
T3 - Methods in Molecular Biology
SP - 19
EP - 28
BT - Campylobacter jejuni
PB - Humana Press
ER -
ID: 184356560