Microfluidic bioreactors for culture of non-adherent cells

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Standard

Microfluidic bioreactors for culture of non-adherent cells. / Shah, Pranjul Jaykumar; Vedarethinam, Indumathi; Kwasny, Dorota; Andresen, Lars; Dimaki, Maria; Skov, Søren; Svendsen, Winnie Edith.

I: Sensors and Actuators B: Chemical, Bind 156, Nr. 2, 2011, s. 1002-1008.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Shah, PJ, Vedarethinam, I, Kwasny, D, Andresen, L, Dimaki, M, Skov, S & Svendsen, WE 2011, 'Microfluidic bioreactors for culture of non-adherent cells', Sensors and Actuators B: Chemical, bind 156, nr. 2, s. 1002-1008. https://doi.org/10.1016/j.snb.2011.02.021

APA

Shah, P. J., Vedarethinam, I., Kwasny, D., Andresen, L., Dimaki, M., Skov, S., & Svendsen, W. E. (2011). Microfluidic bioreactors for culture of non-adherent cells. Sensors and Actuators B: Chemical, 156(2), 1002-1008. https://doi.org/10.1016/j.snb.2011.02.021

Vancouver

Shah PJ, Vedarethinam I, Kwasny D, Andresen L, Dimaki M, Skov S o.a. Microfluidic bioreactors for culture of non-adherent cells. Sensors and Actuators B: Chemical. 2011;156(2):1002-1008. https://doi.org/10.1016/j.snb.2011.02.021

Author

Shah, Pranjul Jaykumar ; Vedarethinam, Indumathi ; Kwasny, Dorota ; Andresen, Lars ; Dimaki, Maria ; Skov, Søren ; Svendsen, Winnie Edith. / Microfluidic bioreactors for culture of non-adherent cells. I: Sensors and Actuators B: Chemical. 2011 ; Bind 156, Nr. 2. s. 1002-1008.

Bibtex

@article{e5ff6d5f6b1840ee92ee445189f7306a,
title = "Microfluidic bioreactors for culture of non-adherent cells",
abstract = "Microfluidic bioreactors (μBR) are becoming increasingly popular for cell culture, sample preparation and analysis in case of routine genetic and clinical diagnostics. We present a novel μBR for non-adherent cells designed to mimic in vivo perfusion of cells based on diffusion of media through a sandwiched membrane. The culture chamber and perfusion chamber are separated by a sandwiched membrane and each chamber has separate inlet/outlets for easy loading/unloading of cells and perfusion of the media. The perfusion of media and exchange of nutrients occur through the sandwiched membrane, which was also verified with simulations. Finally, we present the application of this device for cytogenetic sample preparation, whereby we culture and arrest peripheral T-lymphocytes in metaphase and later fix them in the μBR. The expansion of T-lymphocytes from an unknown patient sample was quantified by means of CFSE staining and subsequent counting in a flow cytometer. To conclude on the applicability of μBR for genetic diagnostics, we prepare chromosome spreads on glass slides from the cultured samples, which is the primary step for metaphase FISH analysis.",
author = "Shah, {Pranjul Jaykumar} and Indumathi Vedarethinam and Dorota Kwasny and Lars Andresen and Maria Dimaki and S{\o}ren Skov and Svendsen, {Winnie Edith}",
year = "2011",
doi = "10.1016/j.snb.2011.02.021",
language = "English",
volume = "156",
pages = "1002--1008",
journal = "Sensors and Actuators B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Microfluidic bioreactors for culture of non-adherent cells

AU - Shah, Pranjul Jaykumar

AU - Vedarethinam, Indumathi

AU - Kwasny, Dorota

AU - Andresen, Lars

AU - Dimaki, Maria

AU - Skov, Søren

AU - Svendsen, Winnie Edith

PY - 2011

Y1 - 2011

N2 - Microfluidic bioreactors (μBR) are becoming increasingly popular for cell culture, sample preparation and analysis in case of routine genetic and clinical diagnostics. We present a novel μBR for non-adherent cells designed to mimic in vivo perfusion of cells based on diffusion of media through a sandwiched membrane. The culture chamber and perfusion chamber are separated by a sandwiched membrane and each chamber has separate inlet/outlets for easy loading/unloading of cells and perfusion of the media. The perfusion of media and exchange of nutrients occur through the sandwiched membrane, which was also verified with simulations. Finally, we present the application of this device for cytogenetic sample preparation, whereby we culture and arrest peripheral T-lymphocytes in metaphase and later fix them in the μBR. The expansion of T-lymphocytes from an unknown patient sample was quantified by means of CFSE staining and subsequent counting in a flow cytometer. To conclude on the applicability of μBR for genetic diagnostics, we prepare chromosome spreads on glass slides from the cultured samples, which is the primary step for metaphase FISH analysis.

AB - Microfluidic bioreactors (μBR) are becoming increasingly popular for cell culture, sample preparation and analysis in case of routine genetic and clinical diagnostics. We present a novel μBR for non-adherent cells designed to mimic in vivo perfusion of cells based on diffusion of media through a sandwiched membrane. The culture chamber and perfusion chamber are separated by a sandwiched membrane and each chamber has separate inlet/outlets for easy loading/unloading of cells and perfusion of the media. The perfusion of media and exchange of nutrients occur through the sandwiched membrane, which was also verified with simulations. Finally, we present the application of this device for cytogenetic sample preparation, whereby we culture and arrest peripheral T-lymphocytes in metaphase and later fix them in the μBR. The expansion of T-lymphocytes from an unknown patient sample was quantified by means of CFSE staining and subsequent counting in a flow cytometer. To conclude on the applicability of μBR for genetic diagnostics, we prepare chromosome spreads on glass slides from the cultured samples, which is the primary step for metaphase FISH analysis.

UR - http://www.scopus.com/inward/record.url?scp=79958146709&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2011.02.021

DO - 10.1016/j.snb.2011.02.021

M3 - Journal article

AN - SCOPUS:79958146709

VL - 156

SP - 1002

EP - 1008

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

SN - 0925-4005

IS - 2

ER -

ID: 49847507