Molecular epidemiology of bovine coronavirus on the basis of comparative analyses of the S gene
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Molecular epidemiology of bovine coronavirus on the basis of comparative analyses of the S gene. / Liu, Lihong; Hägglund, Sara; Hakhverdyan, Mikhayil; Alenius, Stefan; Larsen, Lars Erik; Belák, Sándor.
I: Journal of clinical microbiology, Bind 44, Nr. 3, 03.2006, s. 957-960.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Molecular epidemiology of bovine coronavirus on the basis of comparative analyses of the S gene
AU - Liu, Lihong
AU - Hägglund, Sara
AU - Hakhverdyan, Mikhayil
AU - Alenius, Stefan
AU - Larsen, Lars Erik
AU - Belák, Sándor
PY - 2006/3
Y1 - 2006/3
N2 - Bovine coronaviras (BCoV), a group 2 member of the genus Coronavirus in the family Coronaviridae, is an important pathogen in cattle worldwide. It causes diarrhea in adult animals (winter dysentery), as well as enteric and respiratory diseases in calves. The annual occurrence of BCoV epidemics in Sweden and Denmark led to this investigation, with the aim to deepen the knowledge of BCoV epidemiology at the molecular level. A total of 43 samples from outbreaks in both countries were used for PCR amplification and direct sequencing of a 624-nucleotide fragment of the BCoV S gene. Sequence comparison and phylogenetic studies were performed. The results showed (i) identical sequences from different animals in the same herds and from paired nasal and fecal samples, suggesting a dominant virus circulating in each herd at a given time; (ii) sequence differences among four outbreaks in different years in the same herd, indicating new introduction of virus; (iii) identical sequences in four different Danish herds in samples obtained within 2 months, implying virus transmission between herds; and (iv) that at least two different virus strains were involved in the outbreaks of BCoV in Denmark during the spring of 2003. This study presents molecular data of BCoV infections that will contribute to an increased understanding of BCoV epidemiology in cattle populations.
AB - Bovine coronaviras (BCoV), a group 2 member of the genus Coronavirus in the family Coronaviridae, is an important pathogen in cattle worldwide. It causes diarrhea in adult animals (winter dysentery), as well as enteric and respiratory diseases in calves. The annual occurrence of BCoV epidemics in Sweden and Denmark led to this investigation, with the aim to deepen the knowledge of BCoV epidemiology at the molecular level. A total of 43 samples from outbreaks in both countries were used for PCR amplification and direct sequencing of a 624-nucleotide fragment of the BCoV S gene. Sequence comparison and phylogenetic studies were performed. The results showed (i) identical sequences from different animals in the same herds and from paired nasal and fecal samples, suggesting a dominant virus circulating in each herd at a given time; (ii) sequence differences among four outbreaks in different years in the same herd, indicating new introduction of virus; (iii) identical sequences in four different Danish herds in samples obtained within 2 months, implying virus transmission between herds; and (iv) that at least two different virus strains were involved in the outbreaks of BCoV in Denmark during the spring of 2003. This study presents molecular data of BCoV infections that will contribute to an increased understanding of BCoV epidemiology in cattle populations.
U2 - 10.1128/JCM.44.3.957-960.2006
DO - 10.1128/JCM.44.3.957-960.2006
M3 - Journal article
C2 - 16517883
AN - SCOPUS:33644908835
VL - 44
SP - 957
EP - 960
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
SN - 0095-1137
IS - 3
ER -
ID: 247400248