New reassortant and enzootic european swine influenza viruses transmit efficiently through direct contact in the ferret model
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
New reassortant and enzootic european swine influenza viruses transmit efficiently through direct contact in the ferret model. / Fobian, Kristina; Fabrizio, Thomas P.; Yoon, Sun Woo; Hansen, Mette Sif; Webby, Richard J.; Larsen, Lars E.
I: Journal of General Virology, Bind 96, Nr. 7, 2015, s. 1603-1612.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - New reassortant and enzootic european swine influenza viruses transmit efficiently through direct contact in the ferret model
AU - Fobian, Kristina
AU - Fabrizio, Thomas P.
AU - Yoon, Sun Woo
AU - Hansen, Mette Sif
AU - Webby, Richard J.
AU - Larsen, Lars E.
PY - 2015
Y1 - 2015
N2 - The reverse zoonotic events that introduced the 2009 pandemic influenza virus into pigs have drastically increased the diversity of swine influenza viruses in Europe. The pandemic potential of these novel reassortments is still unclear, necessitating enhanced surveillance of European pigs with additional focus on risk assessment of these new viruses. In this study, four European swine influenza viruses were assessed for their zoonotic potential. Two of the four viruses were enzootic viruses of subtype H1N2 (with avian-like H1) and H3N2, and two were new reassortants, one with avian-like H1 and human-like N2 and one with 2009 pandemic H1 and swine-like N2. All viruses replicated to high titres in nasal wash and nasal turbinate samples from inoculated ferrets and transmitted efficiently by direct contact. Only the H3N2 virus transmitted to naïve ferrets via the airborne route. Growth kinetics using a differentiated human bronchial epithelial cell line showed that all four viruses were able to replicate to high titres. Further, the viruses revealed preferential binding to the 2,6-α-silalylated glycans and investigation of the antiviral susceptibility of the viruses revealed that all were sensitive to neuraminidase inhibitors. These findings suggested that these viruses have the potential to infect humans and further underline the need for continued surveillance as well as biological characterization of new influenza A viruses.
AB - The reverse zoonotic events that introduced the 2009 pandemic influenza virus into pigs have drastically increased the diversity of swine influenza viruses in Europe. The pandemic potential of these novel reassortments is still unclear, necessitating enhanced surveillance of European pigs with additional focus on risk assessment of these new viruses. In this study, four European swine influenza viruses were assessed for their zoonotic potential. Two of the four viruses were enzootic viruses of subtype H1N2 (with avian-like H1) and H3N2, and two were new reassortants, one with avian-like H1 and human-like N2 and one with 2009 pandemic H1 and swine-like N2. All viruses replicated to high titres in nasal wash and nasal turbinate samples from inoculated ferrets and transmitted efficiently by direct contact. Only the H3N2 virus transmitted to naïve ferrets via the airborne route. Growth kinetics using a differentiated human bronchial epithelial cell line showed that all four viruses were able to replicate to high titres. Further, the viruses revealed preferential binding to the 2,6-α-silalylated glycans and investigation of the antiviral susceptibility of the viruses revealed that all were sensitive to neuraminidase inhibitors. These findings suggested that these viruses have the potential to infect humans and further underline the need for continued surveillance as well as biological characterization of new influenza A viruses.
U2 - 10.1099/vir.0.000094
DO - 10.1099/vir.0.000094
M3 - Journal article
C2 - 25701826
AN - SCOPUS:84938346410
VL - 96
SP - 1603
EP - 1612
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 7
ER -
ID: 247396485