Parenteral lipids shape gut bile acid pools and microbiota profiles in the prevention of cholestasis in preterm pigs
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Parenteral lipids shape gut bile acid pools and microbiota profiles in the prevention of cholestasis in preterm pigs. / Call, Lee; Molina, Tiffany; Stoll, Barbara; Guthrie, Greg; Chacko, Shaji; Plat, Jogchum; Robinson, Jason; Lin, Sen; Vonderohe, Caitlin; Mohammad, Mahmoud; Kunichoff, Dennis; Cruz, Stephanie; Lau, Patricio; Premkumar, Muralidhar; Nielsen, Jon; Fang, Zhengfeng; Olutoye, Oluyinka; Thymann, Thomas; Britton, Robert; Sangild, Per; Burrin, Douglas.
I: Journal of Lipid Research, Bind 61, Nr. 7, 2020, s. 1038-1051.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Parenteral lipids shape gut bile acid pools and microbiota profiles in the prevention of cholestasis in preterm pigs
AU - Call, Lee
AU - Molina, Tiffany
AU - Stoll, Barbara
AU - Guthrie, Greg
AU - Chacko, Shaji
AU - Plat, Jogchum
AU - Robinson, Jason
AU - Lin, Sen
AU - Vonderohe, Caitlin
AU - Mohammad, Mahmoud
AU - Kunichoff, Dennis
AU - Cruz, Stephanie
AU - Lau, Patricio
AU - Premkumar, Muralidhar
AU - Nielsen, Jon
AU - Fang, Zhengfeng
AU - Olutoye, Oluyinka
AU - Thymann, Thomas
AU - Britton, Robert
AU - Sangild, Per
AU - Burrin, Douglas
PY - 2020
Y1 - 2020
N2 - Multi-component lipid emulsions, rather than soy-oil emulsions, prevent cholestasis by an unknown mechanism. Here, we quantified liver function, bile acid pools, and gut microbial and metabolite profiles in premature parenterally fed pigs given a soy-oil lipid emulsion, Intralipid (IL), a multi component lipid emulsion, SMOFlipid (SMOF), a novel emulsion with a modified fatty-acid composition [experimental emulsion (EXP)], or a control enteral diet (ENT) for 22 days. We assayed serum cholestasis markers, measured total bile acid levels in plasma, liver, and gut contents, and analyzed colonic bacterial 16S rRNA gene sequences and metabolomic profiles. Serum cholestasis markers (i.e., bilirubin, bile acids, and γ-glutamyl transferase) were highest in IL-fed pigs and normalized in those given SMOF, EXP, or ENT. Gut bile acid pools were lowest in the IL treatment and were increased in the SMOF and EXP treatments and comparable to ENT. Multiple bile acids, especially their conjugated forms, were higher in the colon contents of SMOF and EXP than in IL pigs. The colonic microbial communities of SMOF and EXP pigs had lower relative abundance of several gram-positive anaerobes, including Clostridrium XIVa, and higher abundance of Enterobacteriaceae than those of IL and ENT pigs. Differences in lipid and microbial-derived compounds were also observed in colon metabolite profiles. These results indicate that multi-component lipid emulsions prevent cholestasis and restore enterohepatic bile flow in association with gut microbial and metabolomic changes. We conclude that sustained bile flow induced by multi-component lipid emulsions likely exerts a dominant effect in reducing bile acid-sensitive gram-positive bacteria.
AB - Multi-component lipid emulsions, rather than soy-oil emulsions, prevent cholestasis by an unknown mechanism. Here, we quantified liver function, bile acid pools, and gut microbial and metabolite profiles in premature parenterally fed pigs given a soy-oil lipid emulsion, Intralipid (IL), a multi component lipid emulsion, SMOFlipid (SMOF), a novel emulsion with a modified fatty-acid composition [experimental emulsion (EXP)], or a control enteral diet (ENT) for 22 days. We assayed serum cholestasis markers, measured total bile acid levels in plasma, liver, and gut contents, and analyzed colonic bacterial 16S rRNA gene sequences and metabolomic profiles. Serum cholestasis markers (i.e., bilirubin, bile acids, and γ-glutamyl transferase) were highest in IL-fed pigs and normalized in those given SMOF, EXP, or ENT. Gut bile acid pools were lowest in the IL treatment and were increased in the SMOF and EXP treatments and comparable to ENT. Multiple bile acids, especially their conjugated forms, were higher in the colon contents of SMOF and EXP than in IL pigs. The colonic microbial communities of SMOF and EXP pigs had lower relative abundance of several gram-positive anaerobes, including Clostridrium XIVa, and higher abundance of Enterobacteriaceae than those of IL and ENT pigs. Differences in lipid and microbial-derived compounds were also observed in colon metabolite profiles. These results indicate that multi-component lipid emulsions prevent cholestasis and restore enterohepatic bile flow in association with gut microbial and metabolomic changes. We conclude that sustained bile flow induced by multi-component lipid emulsions likely exerts a dominant effect in reducing bile acid-sensitive gram-positive bacteria.
KW - cholestasis
KW - clostridium
KW - Enterobacteriaceae
KW - fatty acids
KW - liver
KW - metabolomics
KW - microbiome
KW - parenteral nutrition
KW - sterols
U2 - 10.1194/jlr.RA120000652
DO - 10.1194/jlr.RA120000652
M3 - Journal article
C2 - 32350078
AN - SCOPUS:85087469853
VL - 61
SP - 1038
EP - 1051
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 7
ER -
ID: 244615131