Phylogenetic characterisation of the GL sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Phylogenetic characterisation of the GL sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark. / Larsen, Lars E.; Storgaard, Torben; Holm, Elisabeth.

I: Veterinary Microbiology, Bind 80, Nr. 4, 22.06.2001, s. 339-346.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Larsen, LE, Storgaard, T & Holm, E 2001, 'Phylogenetic characterisation of the GL sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark', Veterinary Microbiology, bind 80, nr. 4, s. 339-346. https://doi.org/10.1016/S0378-1135(01)00323-6

APA

Larsen, L. E., Storgaard, T., & Holm, E. (2001). Phylogenetic characterisation of the GL sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark. Veterinary Microbiology, 80(4), 339-346. https://doi.org/10.1016/S0378-1135(01)00323-6

Vancouver

Larsen LE, Storgaard T, Holm E. Phylogenetic characterisation of the GL sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark. Veterinary Microbiology. 2001 jun. 22;80(4):339-346. https://doi.org/10.1016/S0378-1135(01)00323-6

Author

Larsen, Lars E. ; Storgaard, Torben ; Holm, Elisabeth. / Phylogenetic characterisation of the GL sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark. I: Veterinary Microbiology. 2001 ; Bind 80, Nr. 4. s. 339-346.

Bibtex

@article{ff0a30eccbd543de9b75c5239eedc86a,
title = "Phylogenetic characterisation of the GL sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark",
abstract = "The study describes for the first time the phylogenetic relationship between equine arteritis virus (EAV) isolated from asymptomatic virus-shedding stallions and fatal cases of equine viral arteritis (EVA) in an European country. EAV was isolated from three dead foals and an aborted foetus during three different outbreaks of EVA. From these fatalities, the complete open reading frame 5, encoding the EAV GL protein, was amplified by reverse transcription-polymerase chain reaction and subjected to nucleotide sequence analysis. Furthermore, DNA sequences were obtained from virus isolated from semen samples of seven virus-shedding, but clinically healthy, Danish stallions. DNA sequence alignment revealed an overall divergence of 0-14 and 0-10% at the nucleotide and amino acid levels, respectively. Phylogenetic analysis including 24 previously published sequences revealed that European as well as North American {"}types{"} of EAV were present in the semen of asymptomatic carrier stallions and in fatal cases of EVA. Our results reveal that the presence of EAV-shedding stallions in Denmark represents a potential source of severe EVA.",
keywords = "Epidemiology, Equine arteritis virus, G protein, Horse, Open reading frame 5, Phylogeny",
author = "Larsen, {Lars E.} and Torben Storgaard and Elisabeth Holm",
year = "2001",
month = jun,
day = "22",
doi = "10.1016/S0378-1135(01)00323-6",
language = "English",
volume = "80",
pages = "339--346",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - Phylogenetic characterisation of the GL sequences of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis in Denmark

AU - Larsen, Lars E.

AU - Storgaard, Torben

AU - Holm, Elisabeth

PY - 2001/6/22

Y1 - 2001/6/22

N2 - The study describes for the first time the phylogenetic relationship between equine arteritis virus (EAV) isolated from asymptomatic virus-shedding stallions and fatal cases of equine viral arteritis (EVA) in an European country. EAV was isolated from three dead foals and an aborted foetus during three different outbreaks of EVA. From these fatalities, the complete open reading frame 5, encoding the EAV GL protein, was amplified by reverse transcription-polymerase chain reaction and subjected to nucleotide sequence analysis. Furthermore, DNA sequences were obtained from virus isolated from semen samples of seven virus-shedding, but clinically healthy, Danish stallions. DNA sequence alignment revealed an overall divergence of 0-14 and 0-10% at the nucleotide and amino acid levels, respectively. Phylogenetic analysis including 24 previously published sequences revealed that European as well as North American "types" of EAV were present in the semen of asymptomatic carrier stallions and in fatal cases of EVA. Our results reveal that the presence of EAV-shedding stallions in Denmark represents a potential source of severe EVA.

AB - The study describes for the first time the phylogenetic relationship between equine arteritis virus (EAV) isolated from asymptomatic virus-shedding stallions and fatal cases of equine viral arteritis (EVA) in an European country. EAV was isolated from three dead foals and an aborted foetus during three different outbreaks of EVA. From these fatalities, the complete open reading frame 5, encoding the EAV GL protein, was amplified by reverse transcription-polymerase chain reaction and subjected to nucleotide sequence analysis. Furthermore, DNA sequences were obtained from virus isolated from semen samples of seven virus-shedding, but clinically healthy, Danish stallions. DNA sequence alignment revealed an overall divergence of 0-14 and 0-10% at the nucleotide and amino acid levels, respectively. Phylogenetic analysis including 24 previously published sequences revealed that European as well as North American "types" of EAV were present in the semen of asymptomatic carrier stallions and in fatal cases of EVA. Our results reveal that the presence of EAV-shedding stallions in Denmark represents a potential source of severe EVA.

KW - Epidemiology

KW - Equine arteritis virus

KW - G protein

KW - Horse

KW - Open reading frame 5

KW - Phylogeny

U2 - 10.1016/S0378-1135(01)00323-6

DO - 10.1016/S0378-1135(01)00323-6

M3 - Journal article

C2 - 11348770

AN - SCOPUS:0035933680

VL - 80

SP - 339

EP - 346

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 4

ER -

ID: 247400769