Polyamines in spermatocytes and residual bodies of rat testis

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Standard

Polyamines in spermatocytes and residual bodies of rat testis. / Shin, Masashi; Larsson, Lars-Inge; Fujiwara, Kunio.

I: Histochemistry and Cell Biology, Bind 127, Nr. 6, 2007, s. 649-655.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Shin, M, Larsson, L-I & Fujiwara, K 2007, 'Polyamines in spermatocytes and residual bodies of rat testis', Histochemistry and Cell Biology, bind 127, nr. 6, s. 649-655. https://doi.org/10.1007/s00418-007-0285-7

APA

Shin, M., Larsson, L-I., & Fujiwara, K. (2007). Polyamines in spermatocytes and residual bodies of rat testis. Histochemistry and Cell Biology, 127(6), 649-655. https://doi.org/10.1007/s00418-007-0285-7

Vancouver

Shin M, Larsson L-I, Fujiwara K. Polyamines in spermatocytes and residual bodies of rat testis. Histochemistry and Cell Biology. 2007;127(6):649-655. https://doi.org/10.1007/s00418-007-0285-7

Author

Shin, Masashi ; Larsson, Lars-Inge ; Fujiwara, Kunio. / Polyamines in spermatocytes and residual bodies of rat testis. I: Histochemistry and Cell Biology. 2007 ; Bind 127, Nr. 6. s. 649-655.

Bibtex

@article{ba73c6e0a1c211ddb6ae000ea68e967b,
title = "Polyamines in spermatocytes and residual bodies of rat testis",
abstract = "Immunocytochemistry for polyamines in the rat testis revealed intense staining of small bodies close to the lumen of seminiferous tubules of spermatogenic stage VII and VIII as well as of spermatocytes. Methyl green-pyronin and proppidium iodide staining combined with DNase or RNase predigestion showed that the small bodies contained RNA, but not DNA and double fluorescence staining showed that polyamines (PAs) colocalized with RNA in the bodies. Electron microscopy confirmed the absence of nuclei in the bodies and revealed that PA immunoreactivity was associated with ribosomes. These results strongly suggest that the small bodies correspond to the residual bodies, and agree with previous results showing localization of PAs to ribosomes in neurons and gastrointestinal epithelial cells. The accumulation of PAs in residual bodies may reflect a termination of their role in spermiogenesis with respect to protein synthesis.",
keywords = "Former LIFE faculty, Polyamines, Immunocytochemistry, Residual body, Double immunofluorescence, Rat",
author = "Masashi Shin and Lars-Inge Larsson and Kunio Fujiwara",
year = "2007",
doi = "10.1007/s00418-007-0285-7",
language = "English",
volume = "127",
pages = "649--655",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - Polyamines in spermatocytes and residual bodies of rat testis

AU - Shin, Masashi

AU - Larsson, Lars-Inge

AU - Fujiwara, Kunio

PY - 2007

Y1 - 2007

N2 - Immunocytochemistry for polyamines in the rat testis revealed intense staining of small bodies close to the lumen of seminiferous tubules of spermatogenic stage VII and VIII as well as of spermatocytes. Methyl green-pyronin and proppidium iodide staining combined with DNase or RNase predigestion showed that the small bodies contained RNA, but not DNA and double fluorescence staining showed that polyamines (PAs) colocalized with RNA in the bodies. Electron microscopy confirmed the absence of nuclei in the bodies and revealed that PA immunoreactivity was associated with ribosomes. These results strongly suggest that the small bodies correspond to the residual bodies, and agree with previous results showing localization of PAs to ribosomes in neurons and gastrointestinal epithelial cells. The accumulation of PAs in residual bodies may reflect a termination of their role in spermiogenesis with respect to protein synthesis.

AB - Immunocytochemistry for polyamines in the rat testis revealed intense staining of small bodies close to the lumen of seminiferous tubules of spermatogenic stage VII and VIII as well as of spermatocytes. Methyl green-pyronin and proppidium iodide staining combined with DNase or RNase predigestion showed that the small bodies contained RNA, but not DNA and double fluorescence staining showed that polyamines (PAs) colocalized with RNA in the bodies. Electron microscopy confirmed the absence of nuclei in the bodies and revealed that PA immunoreactivity was associated with ribosomes. These results strongly suggest that the small bodies correspond to the residual bodies, and agree with previous results showing localization of PAs to ribosomes in neurons and gastrointestinal epithelial cells. The accumulation of PAs in residual bodies may reflect a termination of their role in spermiogenesis with respect to protein synthesis.

KW - Former LIFE faculty

KW - Polyamines

KW - Immunocytochemistry

KW - Residual body

KW - Double immunofluorescence

KW - Rat

U2 - 10.1007/s00418-007-0285-7

DO - 10.1007/s00418-007-0285-7

M3 - Journal article

C2 - 17443339

VL - 127

SP - 649

EP - 655

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 6

ER -

ID: 8078480