Prediction of Mannheimia haemolytica serotypes based on whole genomic sequences

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The aim of the investigation was to predict the serotypes of M. haemolytica based on whole genomic sequences with the capsular gene region as target. A total of 22 strains selected to have been serotyped and to represent all serotypes were investigated by whole genomic sequencing. The BIGSdb (Bacterial Isolate Genome Sequence Database) was downloaded and installed on a Linux server. Here the sequence database was setup with unique loci at serotype level. The server allows serotypes of M. haemolytica to be predicted from whole genomic sequences and the service is available to the public for free from https://ivsmlst.sund.root.ku.dk.

OriginalsprogEngelsk
Artikelnummer109232
TidsskriftVeterinary Microbiology
Vol/bind262
ISSN0378-1135
DOI
StatusUdgivet - 2021

Bibliografisk note

Funding Information:
Tim Frederik Olsen, Department of Public Health and Kamaran Rahim, SUND-IT University of Copenhagen are thanked for help with the setup and maintainance of BIGSdb. Dr. Keith Jolley, Department of Zoology, University of Oxford is acknowledged for advises with the BIGSdb. Cliff Ramage, Moredun Scientific is acknowledged for gaining access to the reference strain of serotype 17. The project was funded by The Danish Veterinary Consortium and Anicon .

Publisher Copyright:
© 2021 The Authors

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