Prevalence of feline haemoplasma in cats in Denmark
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Prevalence of feline haemoplasma in cats in Denmark. / Rosenqvist, Maja Benedicte; Meilstrup, Ann-Katrine Helene; Larsen, Jesper; Olsen, John Elmerdahl; Jensen, Asger Lundorff; Thomsen, Line Elnif.
I: Acta Veterinaria Scandinavica (Online), Bind 58, 78, 10.11.2016.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Prevalence of feline haemoplasma in cats in Denmark
AU - Rosenqvist, Maja Benedicte
AU - Meilstrup, Ann-Katrine Helene
AU - Larsen, Jesper
AU - Olsen, John Elmerdahl
AU - Jensen, Asger Lundorff
AU - Thomsen, Line Elnif
PY - 2016/11/10
Y1 - 2016/11/10
N2 - Background Infections with the three feline haemotropic mycoplasmas Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis cause feline infectious anemia. The purpose of this study was to investigate the prevalence of carriage of feline haemoplasma in Danish cats in different age groups. The presence was detected by a conventional polymerase chain reaction (PCR) assay on blood samples as well as by real-time PCR (RT-PCR). Results The study revealed a prevalence of 14.9% Candidatus Mycoplasma haemominutum positive cats and 1.5% Mycoplasma haemofelis positive cats. No cats were found positive for Candidatus Mycoplasma turicensis. The results showed a statistically significant higher prevalence in older (>8 years) cats compared to younger cats and a higher prevalence among domestic cats compared to purebred cats. As part of this study, we developed a cloning strategy to obtain Danish positive controls of haemoplasma 16S rRNA. Conclusion From convenience-sampled cats in Denmark, we found that 16.4% were carriers of feline haemotropic mycoplasmas. Haemoplasma was mostly found in older and domestic cats. The prevalence found in Denmark is similar to that found in several other European countries.
AB - Background Infections with the three feline haemotropic mycoplasmas Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis cause feline infectious anemia. The purpose of this study was to investigate the prevalence of carriage of feline haemoplasma in Danish cats in different age groups. The presence was detected by a conventional polymerase chain reaction (PCR) assay on blood samples as well as by real-time PCR (RT-PCR). Results The study revealed a prevalence of 14.9% Candidatus Mycoplasma haemominutum positive cats and 1.5% Mycoplasma haemofelis positive cats. No cats were found positive for Candidatus Mycoplasma turicensis. The results showed a statistically significant higher prevalence in older (>8 years) cats compared to younger cats and a higher prevalence among domestic cats compared to purebred cats. As part of this study, we developed a cloning strategy to obtain Danish positive controls of haemoplasma 16S rRNA. Conclusion From convenience-sampled cats in Denmark, we found that 16.4% were carriers of feline haemotropic mycoplasmas. Haemoplasma was mostly found in older and domestic cats. The prevalence found in Denmark is similar to that found in several other European countries.
KW - Feline haemoplasma
KW - Prevalence
KW - Real-time PCR
KW - Conventional PCR
KW - Risk factors
U2 - 10.1186/s13028-016-0260-1
DO - 10.1186/s13028-016-0260-1
M3 - Journal article
C2 - 27832803
VL - 58
JO - Acta Veterinaria Scandinavica
JF - Acta Veterinaria Scandinavica
SN - 0044-605X
M1 - 78
ER -
ID: 170022968