Liver fluke infection, also known as fasciolosis, is a world-wide prevalent zoonotic parasitic disease infecting a wide range of host species and is caused by Fasciola hepatica. Despite of the substantial economic and animal welfare effects of the disease, knowledge on its prevalence and the factors related to its occurrence and distribution are scarce in Denmark. A retrospective study was performed using liver inspection data of approximately 1.5 million cattle for the period 2011 to 2013. Spatial analysis was carried out to explore whether the data on F. hepatica infection was clustered in space as this could help understand whether or not the infection was driven by local environmental factors. Both global and local spatial autocorrelation techniques were used. Herd level and environmental risk factors were evaluated for their association with prevalence of F. hepatica infection using Generalized Estimating Equations.