Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens

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Standard

Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens. / Fonnesbech, Birte; Frøkiaer, Hanne; Gram, Lone; Jespersen, C. Mosby.

I: Journal of Applied Bacteriology, Bind 74, Nr. 4, 04.1993, s. 444-451.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Fonnesbech, B, Frøkiaer, H, Gram, L & Jespersen, CM 1993, 'Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens', Journal of Applied Bacteriology, bind 74, nr. 4, s. 444-451. https://doi.org/10.1111/j.1365-2672.1993.tb05152.x

APA

Fonnesbech, B., Frøkiaer, H., Gram, L., & Jespersen, C. M. (1993). Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens. Journal of Applied Bacteriology, 74(4), 444-451. https://doi.org/10.1111/j.1365-2672.1993.tb05152.x

Vancouver

Fonnesbech B, Frøkiaer H, Gram L, Jespersen CM. Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens. Journal of Applied Bacteriology. 1993 apr.;74(4):444-451. https://doi.org/10.1111/j.1365-2672.1993.tb05152.x

Author

Fonnesbech, Birte ; Frøkiaer, Hanne ; Gram, Lone ; Jespersen, C. Mosby. / Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens. I: Journal of Applied Bacteriology. 1993 ; Bind 74, Nr. 4. s. 444-451.

Bibtex

@article{7608704db41b4da1861b067cfce4609b,
title = "Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens",
abstract = "B. FONNESBECH, H. FR{\O}KIAER, L. GRAM AND C. MOSBY JESPERSEN. 1993. Polyclonal antibodies were raised in rabbits and mice against Shewanella putrefaciens. Murine monoclonal antibodies were produced against the type strain (ATCC 8071) as well as wild type strains isolated from fish products. The specificities of four polyclonal and 12 monoclonal antibodies were tested by dot‐blotting, an indirect and a competitive ELISA against 16 Gram‐negative strains; including six strains of S. putrefaciens and one strain of Pseudomonas rubescens (NC 10695). All polyclonal antibodies reacted strongly with S. putrefaciens and with Ps. rubescens and cross‐reacted with the nine other bacteria (Pseudomonas spp., Aeromonas spp. and Vibrio anguillarum). The monoclonal antibodies could be divided into three groups with different patterns of specificity. The largest group (8 monoclonal antibodies) reacted strongly with S. putrefaciens and with Ps. rubescens and showed only weak reactions with the other strains. The results confirm that Ps. rubescens should be classified as S. putrefaciens.",
author = "Birte Fonnesbech and Hanne Fr{\o}kiaer and Lone Gram and Jespersen, {C. Mosby}",
year = "1993",
month = apr,
doi = "10.1111/j.1365-2672.1993.tb05152.x",
language = "English",
volume = "74",
pages = "444--451",
journal = "Journal of Applied Bacteriology",
issn = "0021-8847",
publisher = "The Society for Applied Bacteriology",
number = "4",

}

RIS

TY - JOUR

T1 - Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens

AU - Fonnesbech, Birte

AU - Frøkiaer, Hanne

AU - Gram, Lone

AU - Jespersen, C. Mosby

PY - 1993/4

Y1 - 1993/4

N2 - B. FONNESBECH, H. FRØKIAER, L. GRAM AND C. MOSBY JESPERSEN. 1993. Polyclonal antibodies were raised in rabbits and mice against Shewanella putrefaciens. Murine monoclonal antibodies were produced against the type strain (ATCC 8071) as well as wild type strains isolated from fish products. The specificities of four polyclonal and 12 monoclonal antibodies were tested by dot‐blotting, an indirect and a competitive ELISA against 16 Gram‐negative strains; including six strains of S. putrefaciens and one strain of Pseudomonas rubescens (NC 10695). All polyclonal antibodies reacted strongly with S. putrefaciens and with Ps. rubescens and cross‐reacted with the nine other bacteria (Pseudomonas spp., Aeromonas spp. and Vibrio anguillarum). The monoclonal antibodies could be divided into three groups with different patterns of specificity. The largest group (8 monoclonal antibodies) reacted strongly with S. putrefaciens and with Ps. rubescens and showed only weak reactions with the other strains. The results confirm that Ps. rubescens should be classified as S. putrefaciens.

AB - B. FONNESBECH, H. FRØKIAER, L. GRAM AND C. MOSBY JESPERSEN. 1993. Polyclonal antibodies were raised in rabbits and mice against Shewanella putrefaciens. Murine monoclonal antibodies were produced against the type strain (ATCC 8071) as well as wild type strains isolated from fish products. The specificities of four polyclonal and 12 monoclonal antibodies were tested by dot‐blotting, an indirect and a competitive ELISA against 16 Gram‐negative strains; including six strains of S. putrefaciens and one strain of Pseudomonas rubescens (NC 10695). All polyclonal antibodies reacted strongly with S. putrefaciens and with Ps. rubescens and cross‐reacted with the nine other bacteria (Pseudomonas spp., Aeromonas spp. and Vibrio anguillarum). The monoclonal antibodies could be divided into three groups with different patterns of specificity. The largest group (8 monoclonal antibodies) reacted strongly with S. putrefaciens and with Ps. rubescens and showed only weak reactions with the other strains. The results confirm that Ps. rubescens should be classified as S. putrefaciens.

UR - http://www.scopus.com/inward/record.url?scp=0027406219&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2672.1993.tb05152.x

DO - 10.1111/j.1365-2672.1993.tb05152.x

M3 - Journal article

C2 - 8486552

AN - SCOPUS:0027406219

VL - 74

SP - 444

EP - 451

JO - Journal of Applied Bacteriology

JF - Journal of Applied Bacteriology

SN - 0021-8847

IS - 4

ER -

ID: 331795518