Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens
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Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens. / Fonnesbech, Birte; Frøkiaer, Hanne; Gram, Lone; Jespersen, C. Mosby.
I: Journal of Applied Bacteriology, Bind 74, Nr. 4, 04.1993, s. 444-451.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Production and specificity of poly‐ and monoclonal antibodies raised against Shewanella putrefaciens
AU - Fonnesbech, Birte
AU - Frøkiaer, Hanne
AU - Gram, Lone
AU - Jespersen, C. Mosby
PY - 1993/4
Y1 - 1993/4
N2 - B. FONNESBECH, H. FRØKIAER, L. GRAM AND C. MOSBY JESPERSEN. 1993. Polyclonal antibodies were raised in rabbits and mice against Shewanella putrefaciens. Murine monoclonal antibodies were produced against the type strain (ATCC 8071) as well as wild type strains isolated from fish products. The specificities of four polyclonal and 12 monoclonal antibodies were tested by dot‐blotting, an indirect and a competitive ELISA against 16 Gram‐negative strains; including six strains of S. putrefaciens and one strain of Pseudomonas rubescens (NC 10695). All polyclonal antibodies reacted strongly with S. putrefaciens and with Ps. rubescens and cross‐reacted with the nine other bacteria (Pseudomonas spp., Aeromonas spp. and Vibrio anguillarum). The monoclonal antibodies could be divided into three groups with different patterns of specificity. The largest group (8 monoclonal antibodies) reacted strongly with S. putrefaciens and with Ps. rubescens and showed only weak reactions with the other strains. The results confirm that Ps. rubescens should be classified as S. putrefaciens.
AB - B. FONNESBECH, H. FRØKIAER, L. GRAM AND C. MOSBY JESPERSEN. 1993. Polyclonal antibodies were raised in rabbits and mice against Shewanella putrefaciens. Murine monoclonal antibodies were produced against the type strain (ATCC 8071) as well as wild type strains isolated from fish products. The specificities of four polyclonal and 12 monoclonal antibodies were tested by dot‐blotting, an indirect and a competitive ELISA against 16 Gram‐negative strains; including six strains of S. putrefaciens and one strain of Pseudomonas rubescens (NC 10695). All polyclonal antibodies reacted strongly with S. putrefaciens and with Ps. rubescens and cross‐reacted with the nine other bacteria (Pseudomonas spp., Aeromonas spp. and Vibrio anguillarum). The monoclonal antibodies could be divided into three groups with different patterns of specificity. The largest group (8 monoclonal antibodies) reacted strongly with S. putrefaciens and with Ps. rubescens and showed only weak reactions with the other strains. The results confirm that Ps. rubescens should be classified as S. putrefaciens.
UR - http://www.scopus.com/inward/record.url?scp=0027406219&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2672.1993.tb05152.x
DO - 10.1111/j.1365-2672.1993.tb05152.x
M3 - Journal article
C2 - 8486552
AN - SCOPUS:0027406219
VL - 74
SP - 444
EP - 451
JO - Journal of Applied Bacteriology
JF - Journal of Applied Bacteriology
SN - 0021-8847
IS - 4
ER -
ID: 331795518