Protein Concentration Affects the Food Allergen γ-Conglutin Uptake and Bacteria-Induced Cytokine Production in Dendritic Cells
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γ-Conglutin (γ-C) from lupin seeds has been identified as a potent allergen with cross reactivity to peanuts. Here, we investigated how γ-C affected the response in bone marrow-derived dendritic cells (DCs) to bacterial stimuli. γ-C enhanced L. acidophilus NCFM (LaNCFM)-induced IL-12, IL-10, and IL-23 dose-dependently. In contrast, together with E. coli Nissle or LPS, γ-C reduced the production of IL-12 but not of IL-23 and IL-10. Enzyme-hydrolyzed γ-C also enhanced LaNCFM-induced IL-12 and IL-23 production. All preparations induced ROS production in the DCs. The mannose receptor ligands mannan and dextran and the clathrin inhibitor monodansylcadaverine partly inhibited the endocytosis of γ-C. Kunitz trypsin inhibitor and the scavenger receptor ligand polyG also enhanced LaNCFM-induced IL-12, indicating the involvement of receptors other than C-type lectin receptors. The endocytosis of labeled γ-C increased dose-dependently by addition of unlabeled γ-C, which coincided with γ-C’s tendency to aggregate. Taken together, γ-C aggregation affects endocytosis and affects the cytokine production induced by gram-positive and gram-negative bacteria differently. We suggest that γ-C is taken up by the same mechanism as other food proteins but due to aggregation is present in higher concentration in the DCs. This could influence the resulting T-cell response in a microbial stimuli-dependent way.
Originalsprog | Engelsk |
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Artikelnummer | 1531 |
Tidsskrift | Biomolecules |
Vol/bind | 13 |
Udgave nummer | 10 |
Antal sider | 16 |
ISSN | 2218-273X |
DOI | |
Status | Udgivet - 2023 |
Bibliografisk note
Funding Information:
Part of this study was financially supported by the Independent Research Fund Denmark, grant number 1032-00221B, to H.F. The project was partially funded by the “One Health Action Hub: University Task Force for the resilience of territorial ecosystems”, funded by University of Milan (PSR 2021-GSA-Linea 6). G.C.H. was financed by the Ph.D. program in Nutritional Sciences funded by the University of Milan.
Publisher Copyright:
© 2023 by the authors.
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