Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus

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Standard

Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus. / Bojer, Martin Saxtorph; Baldry, Mara; Ingmer, Hanne.

Antimicrobial Peptides: Methods and Protocols. red. / Paul R. Hansen. Bind 1548 Humana Press, 2017. s. 387-394 (Methods in Molecular Biology, Bind 1548).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Bojer, MS, Baldry, M & Ingmer, H 2017, Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus. i PR Hansen (red.), Antimicrobial Peptides: Methods and Protocols. bind 1548, Humana Press, Methods in Molecular Biology, bind 1548, s. 387-394. https://doi.org/10.1007/978-1-4939-6737-7_28

APA

Bojer, M. S., Baldry, M., & Ingmer, H. (2017). Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus. I P. R. Hansen (red.), Antimicrobial Peptides: Methods and Protocols (Bind 1548, s. 387-394). Humana Press. Methods in Molecular Biology Bind 1548 https://doi.org/10.1007/978-1-4939-6737-7_28

Vancouver

Bojer MS, Baldry M, Ingmer H. Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus. I Hansen PR, red., Antimicrobial Peptides: Methods and Protocols. Bind 1548. Humana Press. 2017. s. 387-394. (Methods in Molecular Biology, Bind 1548). https://doi.org/10.1007/978-1-4939-6737-7_28

Author

Bojer, Martin Saxtorph ; Baldry, Mara ; Ingmer, Hanne. / Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus. Antimicrobial Peptides: Methods and Protocols. red. / Paul R. Hansen. Bind 1548 Humana Press, 2017. s. 387-394 (Methods in Molecular Biology, Bind 1548).

Bibtex

@inbook{651e3cadd7024a32bd420a9b81a6afd5,
title = "Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus",
abstract = "Compounds that inhibit virulence gene expression in bacterial pathogens have received increasing interest as possible alternatives to the traditional antibiotic treatment of infections. For the human pathogen Staphylococcus aureus, we have developed two simple assays based on reporter gene fusions to central virulence genes that are easily applicable for screening various sources of natural and synthetic peptides for anti-virulence effects. The plate assay is qualitative but simultaneously assesses the effect of gradient concentrations of the investigated compound, whereas the liquid assay is quantitative and can be employed to address whether a compound is acting on the central quorum sensing regulatory system, agr, that controls a large number of virulence genes in S. aureus.",
keywords = "Agr quorum sensing, Anti-virulence, Compound screening, Hla, Peptides, RNAIII, S. aureus, Spa, β-Galactosidase/β-lactamase reporter genes",
author = "Bojer, {Martin Saxtorph} and Mara Baldry and Hanne Ingmer",
year = "2017",
doi = "10.1007/978-1-4939-6737-7_28",
language = "English",
isbn = "978-1-4939-6735-3",
volume = "1548",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "387--394",
editor = "Hansen, {Paul R.}",
booktitle = "Antimicrobial Peptides",
address = "United States",

}

RIS

TY - CHAP

T1 - Protocols for screening antimicrobial peptides that influence virulence gene expression in Staphylococcus aureus

AU - Bojer, Martin Saxtorph

AU - Baldry, Mara

AU - Ingmer, Hanne

PY - 2017

Y1 - 2017

N2 - Compounds that inhibit virulence gene expression in bacterial pathogens have received increasing interest as possible alternatives to the traditional antibiotic treatment of infections. For the human pathogen Staphylococcus aureus, we have developed two simple assays based on reporter gene fusions to central virulence genes that are easily applicable for screening various sources of natural and synthetic peptides for anti-virulence effects. The plate assay is qualitative but simultaneously assesses the effect of gradient concentrations of the investigated compound, whereas the liquid assay is quantitative and can be employed to address whether a compound is acting on the central quorum sensing regulatory system, agr, that controls a large number of virulence genes in S. aureus.

AB - Compounds that inhibit virulence gene expression in bacterial pathogens have received increasing interest as possible alternatives to the traditional antibiotic treatment of infections. For the human pathogen Staphylococcus aureus, we have developed two simple assays based on reporter gene fusions to central virulence genes that are easily applicable for screening various sources of natural and synthetic peptides for anti-virulence effects. The plate assay is qualitative but simultaneously assesses the effect of gradient concentrations of the investigated compound, whereas the liquid assay is quantitative and can be employed to address whether a compound is acting on the central quorum sensing regulatory system, agr, that controls a large number of virulence genes in S. aureus.

KW - Agr quorum sensing

KW - Anti-virulence

KW - Compound screening

KW - Hla

KW - Peptides

KW - RNAIII

KW - S. aureus

KW - Spa

KW - β-Galactosidase/β-lactamase reporter genes

U2 - 10.1007/978-1-4939-6737-7_28

DO - 10.1007/978-1-4939-6737-7_28

M3 - Book chapter

C2 - 28013520

AN - SCOPUS:85007483207

SN - 978-1-4939-6735-3

VL - 1548

T3 - Methods in Molecular Biology

SP - 387

EP - 394

BT - Antimicrobial Peptides

A2 - Hansen, Paul R.

PB - Humana Press

ER -

ID: 182092202