Reduced virus load in lungs of pigs challenged with porcine reproductive and respiratory syndrome virus after vaccination with virus replicon particles encoding conserved prrsv cytotoxic t-cell epitopes
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Reduced virus load in lungs of pigs challenged with porcine reproductive and respiratory syndrome virus after vaccination with virus replicon particles encoding conserved prrsv cytotoxic t-cell epitopes. / Welner, Simon; Ruggli, Nicolas; Liniger, Matthias; Summerfield, Artur; Larsen, Lars Erik; Jungersen, Gregers.
I: Vaccines, Bind 9, Nr. 3, 208, 2021, s. 1-22.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Reduced virus load in lungs of pigs challenged with porcine reproductive and respiratory syndrome virus after vaccination with virus replicon particles encoding conserved prrsv cytotoxic t-cell epitopes
AU - Welner, Simon
AU - Ruggli, Nicolas
AU - Liniger, Matthias
AU - Summerfield, Artur
AU - Larsen, Lars Erik
AU - Jungersen, Gregers
PY - 2021
Y1 - 2021
N2 - Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe respiratory distress and reproductive failure in swine. Modified live virus (MLV) vaccines provide the highest degree of protection and are most often the preferred choice. While somewhat protective, the use of MLVs is accompanied by multiple safety issues, why safer alternatives are urgently needed. Here, we describe the generation of virus replicon particles (VRPs) based on a classical swine fever virus genome incapable of producing infectious progeny and designed to express conserved PRRSV-2 cytotoxic T-cell epitopes. Eighteen pigs matched with the epitopes by their swine leucocyte antigenprofiles were vaccinated (N = 11, test group) or sham-vaccinated (N = 7, control group) with the VRPs and subsequently challenged with PRRSV-2. The responses to vaccination and challenge were monitored using serological, immunological, and virological analyses. Challenge virus load in serum did not differ significantly between the groups, whereas the virus load in the caudal part of the lung was significantly lower in the test group compared to the control group. The number of peptide-induced interferon-γ secreting cells after challenge was higher and more frequent in the test group than in the control group. Together, our results provide indications of a shapeable PRRSV-specific cell-mediated immune response that may inspire future development of effective PRRSV vaccines.
AB - Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe respiratory distress and reproductive failure in swine. Modified live virus (MLV) vaccines provide the highest degree of protection and are most often the preferred choice. While somewhat protective, the use of MLVs is accompanied by multiple safety issues, why safer alternatives are urgently needed. Here, we describe the generation of virus replicon particles (VRPs) based on a classical swine fever virus genome incapable of producing infectious progeny and designed to express conserved PRRSV-2 cytotoxic T-cell epitopes. Eighteen pigs matched with the epitopes by their swine leucocyte antigenprofiles were vaccinated (N = 11, test group) or sham-vaccinated (N = 7, control group) with the VRPs and subsequently challenged with PRRSV-2. The responses to vaccination and challenge were monitored using serological, immunological, and virological analyses. Challenge virus load in serum did not differ significantly between the groups, whereas the virus load in the caudal part of the lung was significantly lower in the test group compared to the control group. The number of peptide-induced interferon-γ secreting cells after challenge was higher and more frequent in the test group than in the control group. Together, our results provide indications of a shapeable PRRSV-specific cell-mediated immune response that may inspire future development of effective PRRSV vaccines.
KW - Cell-mediated immunity
KW - Classical swine fever virus (CSFV)
KW - Cytotoxic T cells
KW - Polyepitope antigen
KW - Porcine reproductive and respiratory syndrome virus (PRRSV)
KW - Vaccine
KW - Viral vector
KW - Virus replicon particles (VRP)
U2 - 10.3390/vaccines9030208
DO - 10.3390/vaccines9030208
M3 - Journal article
C2 - 33801369
AN - SCOPUS:85102697568
VL - 9
SP - 1
EP - 22
JO - Vaccines
JF - Vaccines
SN - 2076-393X
IS - 3
M1 - 208
ER -
ID: 259101063