TY - JOUR

T1 - Safety and efficacy of l-threonine produced using Escherichia coliCGMCC 13325 as a feed additive for all animal species

AU - Bampidis, Vasileios

AU - Azimonti, Giovanna

AU - Bastos, Maria de Lourdes

AU - Christensen, Henrik

AU - Dusemund, Birgit

AU - Fašmon Durjava, Mojca

AU - Kouba, Maryline

AU - López-Alonso, Marta

AU - López Puente, Secundino

AU - Marcon, Francesca

AU - Mayo, Baltasar

AU - Pechová, Alena

AU - Petkova, Mariana

AU - Ramos, Fernando

AU - Sanz, Yolanda

AU - Villa, Roberto Edoardo

AU - Woutersen, Ruud

AU - Cocconcelli, Pier Sandro

AU - Glandorf, Boet

AU - Herman, Lieve

AU - Maradona, Miguel Prieto

AU - Saarela, Maria

AU - Anguita, Montserrat

AU - Galobart, Jaume

AU - Holczknecht, Orsolya

AU - Manini, Paola

AU - Pettenati, Elisa

AU - Pizzo, Fabiola

AU - Revez, Joana

AU - Tarrés-Call, Jordi

AU - EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP)

PY - 2020

Y1 - 2020

N2 - Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on l-threonine produced by fermentation when used as a nutritional additive in feed and water for drinking for all animal species and categories. The product under assessment is l-threonine produced using a genetically modified strain of E. coliCGMCC 13325. The Panel notes that three out of five batches of the additive do not comply with the minimum specification of 98.5% l-threonine on a dry matter basis proposed by the applicant. The production strain and its DNA were not detected in the final additive. Therefore, the final product does not give raise to any safety concern regarding the genetic modification of the production strain. The use of l-threonine produced using E. coliCGMCC 13325 in supplementing feed to compensate for threonine deficiency in feedingstuffs is safe for the target species. The FEEDAP Panel identified risks of nutritional imbalances and hygienic concerns for amino acids when administered simultaneously in feed and in water for drinking. The use of l-threonine produced by fermentation using E. coliCGMCC 13325 in animal nutrition is considered safe for the consumers and for the environment. There is a risk from the inhalation exposure to endotoxins for persons handling the additive. In the absence of data, the FEEDAP Panel cannot conclude on the potential of l-threonine produced using E. coliCGMCC 13325 to be a skin or eye irritant or a skin sensitiser. The additive under assessment is regarded as an effective source of the amino acid l-threonine for all non-ruminant species. For the supplemental l-threonine to be as efficacious in ruminants as in non-ruminant species, it requires protection against degradation in the rumen.

AB - Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on l-threonine produced by fermentation when used as a nutritional additive in feed and water for drinking for all animal species and categories. The product under assessment is l-threonine produced using a genetically modified strain of E. coliCGMCC 13325. The Panel notes that three out of five batches of the additive do not comply with the minimum specification of 98.5% l-threonine on a dry matter basis proposed by the applicant. The production strain and its DNA were not detected in the final additive. Therefore, the final product does not give raise to any safety concern regarding the genetic modification of the production strain. The use of l-threonine produced using E. coliCGMCC 13325 in supplementing feed to compensate for threonine deficiency in feedingstuffs is safe for the target species. The FEEDAP Panel identified risks of nutritional imbalances and hygienic concerns for amino acids when administered simultaneously in feed and in water for drinking. The use of l-threonine produced by fermentation using E. coliCGMCC 13325 in animal nutrition is considered safe for the consumers and for the environment. There is a risk from the inhalation exposure to endotoxins for persons handling the additive. In the absence of data, the FEEDAP Panel cannot conclude on the potential of l-threonine produced using E. coliCGMCC 13325 to be a skin or eye irritant or a skin sensitiser. The additive under assessment is regarded as an effective source of the amino acid l-threonine for all non-ruminant species. For the supplemental l-threonine to be as efficacious in ruminants as in non-ruminant species, it requires protection against degradation in the rumen.

KW - amino acid

KW - efficacy

KW - Escherichia coliCGMCC 13325

KW - genetically modified organism

KW - l-threonine

KW - nutritional additive

KW - safety

U2 - 10.2903/j.efsa.2020.6332

DO - 10.2903/j.efsa.2020.6332

M3 - Journal article

C2 - 33354234

AN - SCOPUS:85098523393

VL - 18

JO - E F S A Journal

JF - E F S A Journal

SN - 1831-4732

IS - 12

M1 - e06332

ER -