TY - JOUR

T1 - Selection of method is crucial for the diagnosis of porcine circovirus type 2 associated reproductive failures

AU - Hansen, Mette S.

AU - Hjulsager, Charlotte K.

AU - Bille-Hansen, Vivi

AU - Haugegaard, Svend

AU - Dupont, Kitt

AU - Høgedal, Peter

AU - Kunstmann, Lars

AU - Larsen, Lars E.

PY - 2010/7

Y1 - 2010/7

N2 - During a 2-month period a newly repopulated Danish pig herd experienced an increase in numbers of stillborn and mummies, caused by porcine circovirus type 2 (PCV2) associated reproductive failure. Based on recordings of data over time, the progression of the clinical outbreak was studied and the diagnostic value of different techniques was evaluated. Foetal hearts (38 cases and 13 controls) were examined by immunohistochemistry (IHC) and real-time polymerase chain reaction (PCR) for the detection of PCV2; and total immunoglobulin G (IgG) was measured in pleura cavity fluid. PCV2 IHC was positive in 14/38 of the case foetuses, which were delivered during a 9 days period early in the outbreak. On the basis of the results obtained by IHC and PCR, the foetuses were divided into 3 categories: PCV2 negative; moderately positive (104 to 107 copies per 500ng DNA); and massively positive for PCV2 (>107 copies per 500ng DNA). All control- and IHC positive foetuses were included in the negative and massively positive groups, respectively. Ten case foetuses had elevated IgG levels, which did not correlate with the IHC or PCR results. Based on the clustering of the IHC positive foetuses, it is suggested that IHC only is suited for diagnosing acute stages of reproductive failure, whereas quantitative PCR can be used as a sensitive diagnostic method within a wider time span. It seems that IgG measurements are unpredictable as indication of intrauterine infection with PCV2.

AB - During a 2-month period a newly repopulated Danish pig herd experienced an increase in numbers of stillborn and mummies, caused by porcine circovirus type 2 (PCV2) associated reproductive failure. Based on recordings of data over time, the progression of the clinical outbreak was studied and the diagnostic value of different techniques was evaluated. Foetal hearts (38 cases and 13 controls) were examined by immunohistochemistry (IHC) and real-time polymerase chain reaction (PCR) for the detection of PCV2; and total immunoglobulin G (IgG) was measured in pleura cavity fluid. PCV2 IHC was positive in 14/38 of the case foetuses, which were delivered during a 9 days period early in the outbreak. On the basis of the results obtained by IHC and PCR, the foetuses were divided into 3 categories: PCV2 negative; moderately positive (104 to 107 copies per 500ng DNA); and massively positive for PCV2 (>107 copies per 500ng DNA). All control- and IHC positive foetuses were included in the negative and massively positive groups, respectively. Ten case foetuses had elevated IgG levels, which did not correlate with the IHC or PCR results. Based on the clustering of the IHC positive foetuses, it is suggested that IHC only is suited for diagnosing acute stages of reproductive failure, whereas quantitative PCR can be used as a sensitive diagnostic method within a wider time span. It seems that IgG measurements are unpredictable as indication of intrauterine infection with PCV2.

KW - Immunoglobulin G (IgG)

KW - Immunohistochemistry (IHC)

KW - Porcine circovirus type 2 (PCV2) associated reproductive failure

KW - Real-time polymerase chain reaction (PCR)

UR - http://www.scopus.com/inward/record.url?scp=77954814527&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2009.12.038

DO - 10.1016/j.vetmic.2009.12.038

M3 - Journal article

C2 - 20097019

AN - SCOPUS:77954814527

VL - 144

SP - 203

EP - 209

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-2

ER -