Sialic acid-containing milk proteins show differential immunomodulatory activities independent of sialic acid

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Standard

Sialic acid-containing milk proteins show differential immunomodulatory activities independent of sialic acid. / Mikkelsen, Trine L.; Bakman, Susanne; Sørensen, Esben S.; Barkholt, Vibeke; Frøkiær, Hanne.

I: Journal of Agricultural and Food Chemistry, Bind 53, Nr. 20, 05.10.2005, s. 7673-7680.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Mikkelsen, TL, Bakman, S, Sørensen, ES, Barkholt, V & Frøkiær, H 2005, 'Sialic acid-containing milk proteins show differential immunomodulatory activities independent of sialic acid', Journal of Agricultural and Food Chemistry, bind 53, nr. 20, s. 7673-7680. https://doi.org/10.1021/jf050398o

APA

Mikkelsen, T. L., Bakman, S., Sørensen, E. S., Barkholt, V., & Frøkiær, H. (2005). Sialic acid-containing milk proteins show differential immunomodulatory activities independent of sialic acid. Journal of Agricultural and Food Chemistry, 53(20), 7673-7680. https://doi.org/10.1021/jf050398o

Vancouver

Mikkelsen TL, Bakman S, Sørensen ES, Barkholt V, Frøkiær H. Sialic acid-containing milk proteins show differential immunomodulatory activities independent of sialic acid. Journal of Agricultural and Food Chemistry. 2005 okt. 5;53(20):7673-7680. https://doi.org/10.1021/jf050398o

Author

Mikkelsen, Trine L. ; Bakman, Susanne ; Sørensen, Esben S. ; Barkholt, Vibeke ; Frøkiær, Hanne. / Sialic acid-containing milk proteins show differential immunomodulatory activities independent of sialic acid. I: Journal of Agricultural and Food Chemistry. 2005 ; Bind 53, Nr. 20. s. 7673-7680.

Bibtex

@article{902e0bbe3e3e43d083d86b01bc19eacd,
title = "Sialic acid-containing milk proteins show differential immunomodulatory activities independent of sialic acid",
abstract = "The immunomodulatory activities of four sialic acid-containing milk proteins (κ-casein, glycomacropeptide, lactoferrin, and proteose peptone-3 component) were determined, and the role of sialic acid was evaluated. Two in vitro models were used: murine splenocyte proliferation, where the effect on LPS-, Con A-, and PHA-stimulated proliferation was studied, and cytokine production in LPS-stimulated murine dendritic cells (DC). All four proteins inhibited LPS-induced splenocyte proliferation, though to different degrees, and independently of sialic acid. κ-Casein strongly inhibited PHA-induced proliferation and had a weak inhibitory effect on Con A-induced proliferation, whereas lactoferrin stimulated Con A-induced proliferation. κ-Casein, glycomacropeptide, and lactoferrin differentially affected cytokine production by DC: κ-casein significantly inhibited production of TNF-α, IL-10, -12, -6, and -1β, independent of sialic acid, whereas less-marked effects of glycomacropeptide and lactoferrin were seen. These findings thus point to important immunosuppressive effects of some milk proteins and indicate that they may function via different mechanisms.",
keywords = "κ-casein, Cell proliferation, Dendritic cells, Glycomacropeptide, Immunosuppression, Lactoferrin, LPS, PP3, Sialic acid",
author = "Mikkelsen, {Trine L.} and Susanne Bakman and S{\o}rensen, {Esben S.} and Vibeke Barkholt and Hanne Fr{\o}ki{\ae}r",
year = "2005",
month = oct,
day = "5",
doi = "10.1021/jf050398o",
language = "English",
volume = "53",
pages = "7673--7680",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "20",

}

RIS

TY - JOUR

T1 - Sialic acid-containing milk proteins show differential immunomodulatory activities independent of sialic acid

AU - Mikkelsen, Trine L.

AU - Bakman, Susanne

AU - Sørensen, Esben S.

AU - Barkholt, Vibeke

AU - Frøkiær, Hanne

PY - 2005/10/5

Y1 - 2005/10/5

N2 - The immunomodulatory activities of four sialic acid-containing milk proteins (κ-casein, glycomacropeptide, lactoferrin, and proteose peptone-3 component) were determined, and the role of sialic acid was evaluated. Two in vitro models were used: murine splenocyte proliferation, where the effect on LPS-, Con A-, and PHA-stimulated proliferation was studied, and cytokine production in LPS-stimulated murine dendritic cells (DC). All four proteins inhibited LPS-induced splenocyte proliferation, though to different degrees, and independently of sialic acid. κ-Casein strongly inhibited PHA-induced proliferation and had a weak inhibitory effect on Con A-induced proliferation, whereas lactoferrin stimulated Con A-induced proliferation. κ-Casein, glycomacropeptide, and lactoferrin differentially affected cytokine production by DC: κ-casein significantly inhibited production of TNF-α, IL-10, -12, -6, and -1β, independent of sialic acid, whereas less-marked effects of glycomacropeptide and lactoferrin were seen. These findings thus point to important immunosuppressive effects of some milk proteins and indicate that they may function via different mechanisms.

AB - The immunomodulatory activities of four sialic acid-containing milk proteins (κ-casein, glycomacropeptide, lactoferrin, and proteose peptone-3 component) were determined, and the role of sialic acid was evaluated. Two in vitro models were used: murine splenocyte proliferation, where the effect on LPS-, Con A-, and PHA-stimulated proliferation was studied, and cytokine production in LPS-stimulated murine dendritic cells (DC). All four proteins inhibited LPS-induced splenocyte proliferation, though to different degrees, and independently of sialic acid. κ-Casein strongly inhibited PHA-induced proliferation and had a weak inhibitory effect on Con A-induced proliferation, whereas lactoferrin stimulated Con A-induced proliferation. κ-Casein, glycomacropeptide, and lactoferrin differentially affected cytokine production by DC: κ-casein significantly inhibited production of TNF-α, IL-10, -12, -6, and -1β, independent of sialic acid, whereas less-marked effects of glycomacropeptide and lactoferrin were seen. These findings thus point to important immunosuppressive effects of some milk proteins and indicate that they may function via different mechanisms.

KW - κ-casein

KW - Cell proliferation

KW - Dendritic cells

KW - Glycomacropeptide

KW - Immunosuppression

KW - Lactoferrin

KW - LPS

KW - PP3

KW - Sialic acid

UR - http://www.scopus.com/inward/record.url?scp=27144498598&partnerID=8YFLogxK

U2 - 10.1021/jf050398o

DO - 10.1021/jf050398o

M3 - Journal article

C2 - 16190615

AN - SCOPUS:27144498598

VL - 53

SP - 7673

EP - 7680

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 20

ER -

ID: 316998359