TY - JOUR

T1 - Significance and identification of a part of taxon 3 complex of Bisgaard (Gallibacterium genomospecies 3) associated with pigeons

AU - Bisgaard, Magne

AU - Christensen, Henrik

PY - 2019

Y1 - 2019

N2 - Organisms classified with the taxon 2 and taxon 3 complex of Pasteurellaceae have been obtained from lesions in ducks, geese, pigeons, partridges, pheasants and psittacine birds, but can also be isolated from apparently normal birds. We have investigated if biovars of taxon 3 dominated by pigeon isolates belong tothe same species, to outline an unambiguous diagnostic test, and to examine if differences exist between isolates form apparently healthy pigeons and isolates from lesions. A total of 76 isolates from pigeons (n=678) and other birds representing biovars of taxon 3 of Bisgaard were characterized genetically by partialsequencing of the rpoB gene. A monophyletic group including the reference strain of Gallibacterium genomospecies 3 was formed with only up to 2% DNA sequence variation between strains. The group showed from 86 to 87% rpoB gene sequence similarity to the type strain of the type species of genus Gallibacterium (G. anatis). Five strains were classified with other taxa including two strains from pigeons related to the type strain of Pasteurella multocida. A single strain from a parakeet was related to members of the genus Volucribacter while a strain from a partridge showed identical sequence to the reference strain of taxon 14 of Bisgaard. Another strain from a budgerigar was closely related to the reference strain of taxon 44 of Bisgaard. In conclusion, the comparison of partialrpoB sequences obtained from taxon 3 isolates of different biovars obtained from pigeons showed that these strains with two exceptions formed a homogenous genotypic group. Differences were not observed between isolates from lesions and apparently healthy pigeons

AB - Organisms classified with the taxon 2 and taxon 3 complex of Pasteurellaceae have been obtained from lesions in ducks, geese, pigeons, partridges, pheasants and psittacine birds, but can also be isolated from apparently normal birds. We have investigated if biovars of taxon 3 dominated by pigeon isolates belong tothe same species, to outline an unambiguous diagnostic test, and to examine if differences exist between isolates form apparently healthy pigeons and isolates from lesions. A total of 76 isolates from pigeons (n=678) and other birds representing biovars of taxon 3 of Bisgaard were characterized genetically by partialsequencing of the rpoB gene. A monophyletic group including the reference strain of Gallibacterium genomospecies 3 was formed with only up to 2% DNA sequence variation between strains. The group showed from 86 to 87% rpoB gene sequence similarity to the type strain of the type species of genus Gallibacterium (G. anatis). Five strains were classified with other taxa including two strains from pigeons related to the type strain of Pasteurella multocida. A single strain from a parakeet was related to members of the genus Volucribacter while a strain from a partridge showed identical sequence to the reference strain of taxon 14 of Bisgaard. Another strain from a budgerigar was closely related to the reference strain of taxon 44 of Bisgaard. In conclusion, the comparison of partialrpoB sequences obtained from taxon 3 isolates of different biovars obtained from pigeons showed that these strains with two exceptions formed a homogenous genotypic group. Differences were not observed between isolates from lesions and apparently healthy pigeons

KW - Sepsis

KW - phylogeny

KW - diagnostics

U2 - 10.2376/0005-9366-18039

DO - 10.2376/0005-9366-18039

M3 - Journal article

VL - 132

SP - 360

EP - 366

JO - Berliner und Muenchener Tieraerztliche Wochenschrift

JF - Berliner und Muenchener Tieraerztliche Wochenschrift

SN - 0005-9366

IS - 7-8

ER -