Staphylococcus aureus infected embolic stroke upregulates Orm1 and Cxcl2 in a rat model of septic stroke pathology
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Staphylococcus aureus infected embolic stroke upregulates Orm1 and Cxcl2 in a rat model of septic stroke pathology. / Astrup, Lærke Boye; Skovgaard, Kerstin; Rasmussen, Rune Skovgaard; Iburg, Tine Moesgaard; Agerholm, Jørgen Steen; Aalbæk, Bent; Jensen, Henrik Elvang; Nielsen, Ole Lerberg; Johansen, Flemming Fryd; Heegaard, Peter Mikael Helweg; Leifsson, Páll Skúli.
I: Neurological Research, Bind 41, Nr. 5, 2019, s. 399-412.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Staphylococcus aureus infected embolic stroke upregulates Orm1 and Cxcl2 in a rat model of septic stroke pathology
AU - Astrup, Lærke Boye
AU - Skovgaard, Kerstin
AU - Rasmussen, Rune Skovgaard
AU - Iburg, Tine Moesgaard
AU - Agerholm, Jørgen Steen
AU - Aalbæk, Bent
AU - Jensen, Henrik Elvang
AU - Nielsen, Ole Lerberg
AU - Johansen, Flemming Fryd
AU - Heegaard, Peter Mikael Helweg
AU - Leifsson, Páll Skúli
PY - 2019
Y1 - 2019
N2 - Objective: Ischaemic brain lesions and brain abscesses are frequent in both human and animal cases of septic embolic stroke. However, existing models of brain infection do not reflect central aspects of septic embolic stroke. Our aim was to compare septic and non-septic embolic stroke in order to identify gene expressions, inflammatory mediators and brain damage in a rat model. Methods: We created precisely located focal brain infarcts in a rat model of Staphylococcus aureus infected embolic stroke. To cause septic embolic stroke we used a fibrin-rich embolus with bacteria, while every rat in the control group received a non-infected embolus. 64 rats were randomized to receive sham-surgery, sterile embolic stroke or septic embolic stroke. All groups were compared for brain pathology, mortality, gene expressions and inflammatory mediators using histology and reverse transcription quantitative real-time PCR. Results: Although infarct volumes did not differ, septic embolic stroke caused higher mortality than sterile embolic stroke (p= 0.002). Brain abscesses were observed only in the septic group. Approximately 400–500 fold increases were observed for Orm1 and Cxcl2 respectively (1.00E-08 < p < 1.92E-07) in the septic group compared to the sterile group, and these were the most dramatically regulated genes in septic embolic stroke compared to sterile embolic stroke. Conclusions: Septic embolic stroke caused brain abscesses, increased mortality and upregulated Orm1 and Cxcl2 gene expressions compared to non-infected embolic stroke. The dramatic Orm1 increase observed in the septic group is unprecedented and suggests a significant biological role of Orm1 during septic neuroinflammation.
AB - Objective: Ischaemic brain lesions and brain abscesses are frequent in both human and animal cases of septic embolic stroke. However, existing models of brain infection do not reflect central aspects of septic embolic stroke. Our aim was to compare septic and non-septic embolic stroke in order to identify gene expressions, inflammatory mediators and brain damage in a rat model. Methods: We created precisely located focal brain infarcts in a rat model of Staphylococcus aureus infected embolic stroke. To cause septic embolic stroke we used a fibrin-rich embolus with bacteria, while every rat in the control group received a non-infected embolus. 64 rats were randomized to receive sham-surgery, sterile embolic stroke or septic embolic stroke. All groups were compared for brain pathology, mortality, gene expressions and inflammatory mediators using histology and reverse transcription quantitative real-time PCR. Results: Although infarct volumes did not differ, septic embolic stroke caused higher mortality than sterile embolic stroke (p= 0.002). Brain abscesses were observed only in the septic group. Approximately 400–500 fold increases were observed for Orm1 and Cxcl2 respectively (1.00E-08 < p < 1.92E-07) in the septic group compared to the sterile group, and these were the most dramatically regulated genes in septic embolic stroke compared to sterile embolic stroke. Conclusions: Septic embolic stroke caused brain abscesses, increased mortality and upregulated Orm1 and Cxcl2 gene expressions compared to non-infected embolic stroke. The dramatic Orm1 increase observed in the septic group is unprecedented and suggests a significant biological role of Orm1 during septic neuroinflammation.
KW - abscess
KW - animal model
KW - Embolic stroke
KW - gene expressions
KW - neuroinflammation
KW - septic
U2 - 10.1080/01616412.2019.1573455
DO - 10.1080/01616412.2019.1573455
M3 - Journal article
C2 - 30707086
AN - SCOPUS:85060914084
VL - 41
SP - 399
EP - 412
JO - Neurological Research
JF - Neurological Research
SN - 0161-6412
IS - 5
ER -
ID: 216929303