The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature

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The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature. / Ciges-Tomas, J Rafael; Alite, Christian; Humphrey, Suzanne; Donderis, J; Bowring, Janine; Salvatella, Xavier; Penadés, José R; Marina, Alberto.

I: Nature Communications, Bind 10, Nr. 1, 2019, s. 3676.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ciges-Tomas, JR, Alite, C, Humphrey, S, Donderis, J, Bowring, J, Salvatella, X, Penadés, JR & Marina, A 2019, 'The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature', Nature Communications, bind 10, nr. 1, s. 3676. https://doi.org/10.1038/s41467-019-11504-2

APA

Ciges-Tomas, J. R., Alite, C., Humphrey, S., Donderis, J., Bowring, J., Salvatella, X., Penadés, J. R., & Marina, A. (2019). The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature. Nature Communications, 10(1), 3676. https://doi.org/10.1038/s41467-019-11504-2

Vancouver

Ciges-Tomas JR, Alite C, Humphrey S, Donderis J, Bowring J, Salvatella X o.a. The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature. Nature Communications. 2019;10(1):3676. https://doi.org/10.1038/s41467-019-11504-2

Author

Ciges-Tomas, J Rafael ; Alite, Christian ; Humphrey, Suzanne ; Donderis, J ; Bowring, Janine ; Salvatella, Xavier ; Penadés, José R ; Marina, Alberto. / The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature. I: Nature Communications. 2019 ; Bind 10, Nr. 1. s. 3676.

Bibtex

@article{2d275d9b888040b19de694d149f76881,
title = "The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature",
abstract = "Stl is a master repressor encoded by Staphylococcus aureus pathogenicity islands (SaPIs) that maintains integration of these elements in the bacterial chromosome. After infection or induction of a resident helper phage, SaPIs are de-repressed by specific interactions of phage proteins with Stl. SaPIs have evolved a fascinating mechanism to ensure their promiscuous transfer by targeting structurally unrelated proteins performing identically conserved functions for the phage. Here we decipher the molecular mechanism of this elegant strategy by determining the structure of SaPIbov1 Stl alone and in complex with two structurally unrelated dUTPases from different S. aureus phages. Remarkably, SaPIbov1 Stl has evolved different domains implicated in DNA and partner recognition specificity. This work presents the solved structure of a SaPI repressor protein and the discovery of a modular repressor that acquires multispecificity through domain recruiting. Our results establish the mechanism that allows widespread dissemination of SaPIs in nature.",
keywords = "Biological Coevolution, Crystallography, X-Ray, DNA-Binding Proteins/genetics, Genomic Islands/genetics, Models, Molecular, Staphylococcus Phages/genetics, Staphylococcus aureus/genetics",
author = "Ciges-Tomas, {J Rafael} and Christian Alite and Suzanne Humphrey and J Donderis and Janine Bowring and Xavier Salvatella and Penad{\'e}s, {Jos{\'e} R} and Alberto Marina",
year = "2019",
doi = "10.1038/s41467-019-11504-2",
language = "English",
volume = "10",
pages = "3676",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - The structure of a polygamous repressor reveals how phage-inducible chromosomal islands spread in nature

AU - Ciges-Tomas, J Rafael

AU - Alite, Christian

AU - Humphrey, Suzanne

AU - Donderis, J

AU - Bowring, Janine

AU - Salvatella, Xavier

AU - Penadés, José R

AU - Marina, Alberto

PY - 2019

Y1 - 2019

N2 - Stl is a master repressor encoded by Staphylococcus aureus pathogenicity islands (SaPIs) that maintains integration of these elements in the bacterial chromosome. After infection or induction of a resident helper phage, SaPIs are de-repressed by specific interactions of phage proteins with Stl. SaPIs have evolved a fascinating mechanism to ensure their promiscuous transfer by targeting structurally unrelated proteins performing identically conserved functions for the phage. Here we decipher the molecular mechanism of this elegant strategy by determining the structure of SaPIbov1 Stl alone and in complex with two structurally unrelated dUTPases from different S. aureus phages. Remarkably, SaPIbov1 Stl has evolved different domains implicated in DNA and partner recognition specificity. This work presents the solved structure of a SaPI repressor protein and the discovery of a modular repressor that acquires multispecificity through domain recruiting. Our results establish the mechanism that allows widespread dissemination of SaPIs in nature.

AB - Stl is a master repressor encoded by Staphylococcus aureus pathogenicity islands (SaPIs) that maintains integration of these elements in the bacterial chromosome. After infection or induction of a resident helper phage, SaPIs are de-repressed by specific interactions of phage proteins with Stl. SaPIs have evolved a fascinating mechanism to ensure their promiscuous transfer by targeting structurally unrelated proteins performing identically conserved functions for the phage. Here we decipher the molecular mechanism of this elegant strategy by determining the structure of SaPIbov1 Stl alone and in complex with two structurally unrelated dUTPases from different S. aureus phages. Remarkably, SaPIbov1 Stl has evolved different domains implicated in DNA and partner recognition specificity. This work presents the solved structure of a SaPI repressor protein and the discovery of a modular repressor that acquires multispecificity through domain recruiting. Our results establish the mechanism that allows widespread dissemination of SaPIs in nature.

KW - Biological Coevolution

KW - Crystallography, X-Ray

KW - DNA-Binding Proteins/genetics

KW - Genomic Islands/genetics

KW - Models, Molecular

KW - Staphylococcus Phages/genetics

KW - Staphylococcus aureus/genetics

U2 - 10.1038/s41467-019-11504-2

DO - 10.1038/s41467-019-11504-2

M3 - Journal article

C2 - 31417084

VL - 10

SP - 3676

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

ER -

ID: 373881907