The use of antibodies for characterization and quantification of a recombinant protein
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
In this study, we characterized proteinase A secreted by recombinant Saccharomyces cerevisiae bearing a multicopy plasmid containing the encoding gene (PEP4). Polyclonal and monoclonal antibodies were raised to study the product heterogeneity. Characterization of proteinase A was performed by immunoelectrophoresis and immunoblotting techniques. None of the monoclonal antibodies raised against proteinase A was found to react with the glycosyl side chains; thus cross-reaction with other glycosylated proteins (e.g. carboxypeptidase Y) was very low. This study allowed us to develop an ELISA method for the quantification of proteinase A in culture supernatants as well as the evaluation of monoclonal antibodies for their use in immunoaffinity chromatography.
Originalsprog | Engelsk |
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Tidsskrift | Annals of the New York Academy of Sciences |
Vol/bind | 782 |
Sider (fra-til) | 462-477 |
Antal sider | 16 |
ISSN | 0077-8923 |
DOI | |
Status | Udgivet - 1996 |
ID: 331794754