Transcription levels of endothelin-1 and endothelin receptors are associated with age and leaflet location in porcine mitral valves
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Transcription levels of endothelin-1 and endothelin receptors are associated with age and leaflet location in porcine mitral valves. / Pedersen, Lotte Gam; Offenberg, Hanne Kjær; Moesgaard, Sophia Gry; Thomsen, Preben Dybdahl; Pedersen, H.D.; Olsen, Lisbeth Høier.
I: Zoonoses and Public Health, Bind 54, Nr. 3, 2007, s. 113-118.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Transcription levels of endothelin-1 and endothelin receptors are associated with age and leaflet location in porcine mitral valves
AU - Pedersen, Lotte Gam
AU - Offenberg, Hanne Kjær
AU - Moesgaard, Sophia Gry
AU - Thomsen, Preben Dybdahl
AU - Pedersen, H.D.
AU - Olsen, Lisbeth Høier
PY - 2007
Y1 - 2007
N2 - The aim of the study was to investigate the expression levels of endothelin-1 (ET-1) and ETA and ETB receptors (ETA-R and ETB-R) in porcine mitral valves and associate the transcription levels to age, leaflet location and deposition of mucopolysaccharides (MPS). Tissue samples from the chordal and interchordal insertion area of the anterior mitral valve leaflet from 11 sows (=2 years of age) and 10 slaughter pigs (approximately 6 months old) were obtained and the relative gene expression levels of ET-1, ETA-R and ETB-R measured by semi-quantitative real-time PCR. A separate tissue sample was taken for histopathological grading of MPS deposition. The transcription levels of ET-1 (P < 0.0001) and ETA-R (P < 0.0004) were significantly higher in leaflets from the sows compared with slaughter pigs. The gene expression of ETB-R was not associated to age (P = 0.38), but increased in chordal insertion areas compared with inter-chordal areas (P = 0.01). The expression of ET-1 and ETA-R mRNA did not differ significantly between the two leaflet locations. The valve leaflets from sows had a significantly increased degree of MPS deposition compared with slaughter pigs upon histological examination (P = 0.04). In conclusion, an age-related valvular degeneration is observed in porcien mitral valve leaflets and ET-1 is suggested to be involved through action of both ETA and ETB receptors.
AB - The aim of the study was to investigate the expression levels of endothelin-1 (ET-1) and ETA and ETB receptors (ETA-R and ETB-R) in porcine mitral valves and associate the transcription levels to age, leaflet location and deposition of mucopolysaccharides (MPS). Tissue samples from the chordal and interchordal insertion area of the anterior mitral valve leaflet from 11 sows (=2 years of age) and 10 slaughter pigs (approximately 6 months old) were obtained and the relative gene expression levels of ET-1, ETA-R and ETB-R measured by semi-quantitative real-time PCR. A separate tissue sample was taken for histopathological grading of MPS deposition. The transcription levels of ET-1 (P < 0.0001) and ETA-R (P < 0.0004) were significantly higher in leaflets from the sows compared with slaughter pigs. The gene expression of ETB-R was not associated to age (P = 0.38), but increased in chordal insertion areas compared with inter-chordal areas (P = 0.01). The expression of ET-1 and ETA-R mRNA did not differ significantly between the two leaflet locations. The valve leaflets from sows had a significantly increased degree of MPS deposition compared with slaughter pigs upon histological examination (P = 0.04). In conclusion, an age-related valvular degeneration is observed in porcien mitral valve leaflets and ET-1 is suggested to be involved through action of both ETA and ETB receptors.
U2 - 10.1111/j.1439-0442.2007.00894.x
DO - 10.1111/j.1439-0442.2007.00894.x
M3 - Journal article
C2 - 17381672
VL - 54
SP - 113
EP - 118
JO - Zoonoses and Public Health
JF - Zoonoses and Public Health
SN - 1863-1959
IS - 3
ER -
ID: 8065591