Ultrastructure and mitochondrial numbers in pre- and postpubertal pig oocytes
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Ultrastructure and mitochondrial numbers in pre- and postpubertal pig oocytes. / Pedersen, Hanne Skovsgaard; Callesen, Henrik; Løvendahl, Peter; Chen, Fenghua; Nyengaard, Jens Randel; Nikolaisen, Nanett Kvist; Holm, Peter; Hyttel, Poul.
I: Reproduction, Fertility and Development, Bind 28, Nr. 5, 2016, s. 586-598.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Ultrastructure and mitochondrial numbers in pre- and postpubertal pig oocytes
AU - Pedersen, Hanne Skovsgaard
AU - Callesen, Henrik
AU - Løvendahl, Peter
AU - Chen, Fenghua
AU - Nyengaard, Jens Randel
AU - Nikolaisen, Nanett Kvist
AU - Holm, Peter
AU - Hyttel, Poul
PY - 2016
Y1 - 2016
N2 - Prepubertal pig oocytes are associated with lower developmental competence. The aim of this experiment was to conduct an exhaustive survey of oocyte ultrastructure and to use a design-unbiased stereological approach to quantify the numerical density and total number of mitochondria in oocytes with different diameters from pre- and postpubertal pigs. The ultrastructure of smaller prepubertal immature oocytes indicated active cells in close contact with cumulus cells. The postpubertal oocytes were more quiescent cell types. The small prepubertal oocytes had a lower total mitochondrial number, but no differences were observed in mitochondrial densities between groups. Mature postpubertal oocytes adhered to the following characteristics: presence of metaphase II, lack of contact between cumulus cells and oocyte, absence of rough endoplasmic reticulum and Golgi complexes, peripheral location of cortical granules and central localisation of mitochondria, vesicles and lipid droplets. Prepubertal oocytes displayed more variation. The ultrastructure of large pre- and postpubertal oocytes was compatible with higher developmental competence, whereas that of smaller prepubertal oocytes could explain their reduced capacity. The higher number of mitochondria in large pre- and postpubertal oocytes could have an influence on oocyte competence, by increasing the pool of mitochondria available for early embryonic development.
AB - Prepubertal pig oocytes are associated with lower developmental competence. The aim of this experiment was to conduct an exhaustive survey of oocyte ultrastructure and to use a design-unbiased stereological approach to quantify the numerical density and total number of mitochondria in oocytes with different diameters from pre- and postpubertal pigs. The ultrastructure of smaller prepubertal immature oocytes indicated active cells in close contact with cumulus cells. The postpubertal oocytes were more quiescent cell types. The small prepubertal oocytes had a lower total mitochondrial number, but no differences were observed in mitochondrial densities between groups. Mature postpubertal oocytes adhered to the following characteristics: presence of metaphase II, lack of contact between cumulus cells and oocyte, absence of rough endoplasmic reticulum and Golgi complexes, peripheral location of cortical granules and central localisation of mitochondria, vesicles and lipid droplets. Prepubertal oocytes displayed more variation. The ultrastructure of large pre- and postpubertal oocytes was compatible with higher developmental competence, whereas that of smaller prepubertal oocytes could explain their reduced capacity. The higher number of mitochondria in large pre- and postpubertal oocytes could have an influence on oocyte competence, by increasing the pool of mitochondria available for early embryonic development.
U2 - 10.1071/RD14220
DO - 10.1071/RD14220
M3 - Journal article
C2 - 25482576
VL - 28
SP - 586
EP - 598
JO - Australian journal of scientific research. Ser. B: Biological sciences
JF - Australian journal of scientific research. Ser. B: Biological sciences
SN - 1031-3613
IS - 5
ER -
ID: 131490196