CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis. / Kohm, Katharina; Lutz, Veronika Theresa; Friedrich, Ines; Hertel, Robert.

Metagenomics: Methods and Protocols. ed. / Wolfgang R. Streit; Rolf Daniel. Humana Press, 2023. p. 205-212 (Methods in Molecular Biology, Vol. 2555).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Kohm, K, Lutz, VT, Friedrich, I & Hertel, R 2023, CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis. in WR Streit & R Daniel (eds), Metagenomics: Methods and Protocols. Humana Press, Methods in Molecular Biology, vol. 2555, pp. 205-212. https://doi.org/10.1007/978-1-0716-2795-2_15

APA

Kohm, K., Lutz, V. T., Friedrich, I., & Hertel, R. (2023). CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis. In W. R. Streit, & R. Daniel (Eds.), Metagenomics: Methods and Protocols (pp. 205-212). Humana Press. Methods in Molecular Biology Vol. 2555 https://doi.org/10.1007/978-1-0716-2795-2_15

Vancouver

Kohm K, Lutz VT, Friedrich I, Hertel R. CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis. In Streit WR, Daniel R, editors, Metagenomics: Methods and Protocols. Humana Press. 2023. p. 205-212. (Methods in Molecular Biology, Vol. 2555). https://doi.org/10.1007/978-1-0716-2795-2_15

Author

Kohm, Katharina ; Lutz, Veronika Theresa ; Friedrich, Ines ; Hertel, Robert. / CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis. Metagenomics: Methods and Protocols. editor / Wolfgang R. Streit ; Rolf Daniel. Humana Press, 2023. pp. 205-212 (Methods in Molecular Biology, Vol. 2555).

Bibtex

@inbook{1e40315f2f33460e9c297bd85991de53,
title = "CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis",
abstract = "Phages are viruses of bacteria and have been known for over a century. They do not have a metabolism or protein synthesis machinery and rely on host cells for replication. The model organism Bacillus subtilis has served as a host strain for decades and enabled the isolation of many unique viral strains. However, many viral species representatives remained orphans as no, or only a few, related phages were ever re-isolated. The presented protocol describes how a CRISPR-Cas9 system with an artificial CRISPR-array can be set up and used to discriminate abundant and well-known B. subtilis phage from a host-based metagenome enrichment. The obtained viral suspension can be used for metagenome sequencing and isolating new viral strains.",
keywords = "Bacillus subtilis, Bacteriophage, CRISPR-Cas9, CutSPR, gRNA, Metagenome, Phage",
author = "Katharina Kohm and Lutz, {Veronika Theresa} and Ines Friedrich and Robert Hertel",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2795-2_15",
language = "English",
isbn = "978-1-0716-2797-6",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "205--212",
editor = "Streit, {Wolfgang R. } and Daniel, {Rolf }",
booktitle = "Metagenomics",
address = "United States",

}

RIS

TY - CHAP

T1 - CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis

AU - Kohm, Katharina

AU - Lutz, Veronika Theresa

AU - Friedrich, Ines

AU - Hertel, Robert

N1 - Publisher Copyright: © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2023

Y1 - 2023

N2 - Phages are viruses of bacteria and have been known for over a century. They do not have a metabolism or protein synthesis machinery and rely on host cells for replication. The model organism Bacillus subtilis has served as a host strain for decades and enabled the isolation of many unique viral strains. However, many viral species representatives remained orphans as no, or only a few, related phages were ever re-isolated. The presented protocol describes how a CRISPR-Cas9 system with an artificial CRISPR-array can be set up and used to discriminate abundant and well-known B. subtilis phage from a host-based metagenome enrichment. The obtained viral suspension can be used for metagenome sequencing and isolating new viral strains.

AB - Phages are viruses of bacteria and have been known for over a century. They do not have a metabolism or protein synthesis machinery and rely on host cells for replication. The model organism Bacillus subtilis has served as a host strain for decades and enabled the isolation of many unique viral strains. However, many viral species representatives remained orphans as no, or only a few, related phages were ever re-isolated. The presented protocol describes how a CRISPR-Cas9 system with an artificial CRISPR-array can be set up and used to discriminate abundant and well-known B. subtilis phage from a host-based metagenome enrichment. The obtained viral suspension can be used for metagenome sequencing and isolating new viral strains.

KW - Bacillus subtilis

KW - Bacteriophage

KW - CRISPR-Cas9

KW - CutSPR

KW - gRNA

KW - Metagenome

KW - Phage

U2 - 10.1007/978-1-0716-2795-2_15

DO - 10.1007/978-1-0716-2795-2_15

M3 - Book chapter

C2 - 36306089

AN - SCOPUS:85141004031

SN - 978-1-0716-2797-6

T3 - Methods in Molecular Biology

SP - 205

EP - 212

BT - Metagenomics

A2 - Streit, Wolfgang R.

A2 - Daniel, Rolf

PB - Humana Press

ER -

ID: 333617321