Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni. / Gencay, Yilmaz Emre; Birk, Tina; Sørensen, Martine Camilla Holst; Brøndsted, Lone.

Campylobacter jejuni: Methods and Protocols. Vol. 1512 Humana Press, 2017. p. 19-28 (Methods in Molecular Biology, Vol. 1512).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Gencay, YE, Birk, T, Sørensen, MCH & Brøndsted, L 2017, Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni. in Campylobacter jejuni: Methods and Protocols. vol. 1512, Humana Press, Methods in Molecular Biology, vol. 1512, pp. 19-28. https://doi.org/10.1007/978-1-4939-6536-6_3

APA

Gencay, Y. E., Birk, T., Sørensen, M. C. H., & Brøndsted, L. (2017). Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni. In Campylobacter jejuni: Methods and Protocols (Vol. 1512, pp. 19-28). Humana Press. Methods in Molecular Biology Vol. 1512 https://doi.org/10.1007/978-1-4939-6536-6_3

Vancouver

Gencay YE, Birk T, Sørensen MCH, Brøndsted L. Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni. In Campylobacter jejuni: Methods and Protocols. Vol. 1512. Humana Press. 2017. p. 19-28. (Methods in Molecular Biology, Vol. 1512). https://doi.org/10.1007/978-1-4939-6536-6_3

Author

Gencay, Yilmaz Emre ; Birk, Tina ; Sørensen, Martine Camilla Holst ; Brøndsted, Lone. / Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni. Campylobacter jejuni: Methods and Protocols. Vol. 1512 Humana Press, 2017. pp. 19-28 (Methods in Molecular Biology, Vol. 1512).

Bibtex

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title = "Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni",
abstract = "Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.",
keywords = "Bacteriophage, C. jejuni, Campylobacter, Isolation, Propagation, Purification, Titration",
author = "Gencay, {Yilmaz Emre} and Tina Birk and S{\o}rensen, {Martine Camilla Holst} and Lone Br{\o}ndsted",
year = "2017",
doi = "10.1007/978-1-4939-6536-6_3",
language = "English",
isbn = "978-1-4939-6534-2",
volume = "1512",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "19--28",
booktitle = "Campylobacter jejuni",
address = "United States",

}

RIS

TY - CHAP

T1 - Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni

AU - Gencay, Yilmaz Emre

AU - Birk, Tina

AU - Sørensen, Martine Camilla Holst

AU - Brøndsted, Lone

PY - 2017

Y1 - 2017

N2 - Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.

AB - Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.

KW - Bacteriophage

KW - C. jejuni

KW - Campylobacter

KW - Isolation

KW - Propagation

KW - Purification

KW - Titration

U2 - 10.1007/978-1-4939-6536-6_3

DO - 10.1007/978-1-4939-6536-6_3

M3 - Book chapter

C2 - 27885595

AN - SCOPUS:84997235896

SN - 978-1-4939-6534-2

VL - 1512

T3 - Methods in Molecular Biology

SP - 19

EP - 28

BT - Campylobacter jejuni

PB - Humana Press

ER -

ID: 184356560