Methods for isolation, purification, and propagation of bacteriophages of Campylobacter jejuni
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Here, we describe the methods for isolation, purification, and propagation of Campylobacter jejuni bacteriophages from samples expected to contain high number of phages such as chicken feces. The overall steps are (1) liberation of phages from the sample material; (2) observation of plaque-forming units on C. jejuni lawns using a spot assay; (3) isolation of single plaques; (4) consecutive purification procedures; and (5) propagation of purified phages from a plate lysate to prepare master stocks.
Original language | English |
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Title of host publication | Campylobacter jejuni : Methods and Protocols |
Number of pages | 10 |
Volume | 1512 |
Publisher | Humana Press |
Publication date | 2017 |
Pages | 19-28 |
ISBN (Print) | 978-1-4939-6534-2 |
ISBN (Electronic) | 978-1-4939-6536-6 |
DOIs | |
Publication status | Published - 2017 |
Series | Methods in Molecular Biology |
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Volume | 1512 |
ISSN | 1064-3745 |
- Bacteriophage, C. jejuni, Campylobacter, Isolation, Propagation, Purification, Titration
Research areas
ID: 184356560