Genital Infiltrations of CD4(+) and CD8(+) T Lymphocytes, IgA(+) and IgG(+) Plasma Cells and Intra-Mucosal Lymphoid Follicles Associate With Protection Against Genital Chlamydia trachomatis Infection in Minipigs Intramuscularly Immunized With UV-Inactivated Bacteria Adjuvanted With CAF01

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Genital Infiltrations of CD4(+) and CD8(+) T Lymphocytes, IgA(+) and IgG(+) Plasma Cells and Intra-Mucosal Lymphoid Follicles Associate With Protection Against Genital Chlamydia trachomatis Infection in Minipigs Intramuscularly Immunized With UV-Inactivated Bacteria Adjuvanted With CAF01. / Erneholm, Karin; Lorenzen, Emma; Boje, Sarah; Olsen, Anja Weinreich; Jungersen, Gregers; Jensen, Henrik E.; Cassidy, Joseph P.; Andersen, Peter; Agerholm, Jorgen S.; Follmann, Frank.

In: Frontiers in Microbiology, Vol. 10, 197, 2019.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Erneholm, K, Lorenzen, E, Boje, S, Olsen, AW, Jungersen, G, Jensen, HE, Cassidy, JP, Andersen, P, Agerholm, JS & Follmann, F 2019, 'Genital Infiltrations of CD4(+) and CD8(+) T Lymphocytes, IgA(+) and IgG(+) Plasma Cells and Intra-Mucosal Lymphoid Follicles Associate With Protection Against Genital Chlamydia trachomatis Infection in Minipigs Intramuscularly Immunized With UV-Inactivated Bacteria Adjuvanted With CAF01', Frontiers in Microbiology, vol. 10, 197. https://doi.org/10.3389/fmicb.2019.00197

APA

Erneholm, K., Lorenzen, E., Boje, S., Olsen, A. W., Jungersen, G., Jensen, H. E., Cassidy, J. P., Andersen, P., Agerholm, J. S., & Follmann, F. (2019). Genital Infiltrations of CD4(+) and CD8(+) T Lymphocytes, IgA(+) and IgG(+) Plasma Cells and Intra-Mucosal Lymphoid Follicles Associate With Protection Against Genital Chlamydia trachomatis Infection in Minipigs Intramuscularly Immunized With UV-Inactivated Bacteria Adjuvanted With CAF01. Frontiers in Microbiology, 10, [197]. https://doi.org/10.3389/fmicb.2019.00197

Vancouver

Erneholm K, Lorenzen E, Boje S, Olsen AW, Jungersen G, Jensen HE et al. Genital Infiltrations of CD4(+) and CD8(+) T Lymphocytes, IgA(+) and IgG(+) Plasma Cells and Intra-Mucosal Lymphoid Follicles Associate With Protection Against Genital Chlamydia trachomatis Infection in Minipigs Intramuscularly Immunized With UV-Inactivated Bacteria Adjuvanted With CAF01. Frontiers in Microbiology. 2019;10. 197. https://doi.org/10.3389/fmicb.2019.00197

Author

Erneholm, Karin ; Lorenzen, Emma ; Boje, Sarah ; Olsen, Anja Weinreich ; Jungersen, Gregers ; Jensen, Henrik E. ; Cassidy, Joseph P. ; Andersen, Peter ; Agerholm, Jorgen S. ; Follmann, Frank. / Genital Infiltrations of CD4(+) and CD8(+) T Lymphocytes, IgA(+) and IgG(+) Plasma Cells and Intra-Mucosal Lymphoid Follicles Associate With Protection Against Genital Chlamydia trachomatis Infection in Minipigs Intramuscularly Immunized With UV-Inactivated Bacteria Adjuvanted With CAF01. In: Frontiers in Microbiology. 2019 ; Vol. 10.

Bibtex

@article{42cbdd856d5b4ed1ac35e68170f97cc5,
title = "Genital Infiltrations of CD4(+) and CD8(+) T Lymphocytes, IgA(+) and IgG(+) Plasma Cells and Intra-Mucosal Lymphoid Follicles Associate With Protection Against Genital Chlamydia trachomatis Infection in Minipigs Intramuscularly Immunized With UV-Inactivated Bacteria Adjuvanted With CAF01",
abstract = "The development of a vaccine against genital chlamydia in women is advancing, and the evaluation of in situ immune responses following vaccination and challenge infections is crucial for development of a safe and protective vaccine. This study employs the sexually mature minipig model to characterize the genital in situ immune response to Chlamydia trachomatis infection in pigs previously immunized intramuscularly with UV-inactivated C. trachomatis serovar D (UV-SvD) adjuvanted/formulated with CAF01 adjuvant compared to a CAF01-alone control group. Pigs immunized with UV-SvD were significantly protected against vaginal challenge with C. trachomatis on day 3 post inoculation and showed significantly higher cervical infiltrations of approximately equal numbers of CD4+ and CD8+ T-cells, and IgG+ and IgA+ plasma cells compared to adjuvant-alone immunized controls. These immunological signatures correspond to findings in mice and are similar to those described in female chlamydia patients. This proves important potential for the pig model in elucidating immunological in situ signatures in future translational research in chlamydia vaccinology.",
keywords = "porcine, chlamydia, pathology, histology, vaccine",
author = "Karin Erneholm and Emma Lorenzen and Sarah Boje and Olsen, {Anja Weinreich} and Gregers Jungersen and Jensen, {Henrik E.} and Cassidy, {Joseph P.} and Peter Andersen and Agerholm, {Jorgen S.} and Frank Follmann",
year = "2019",
doi = "10.3389/fmicb.2019.00197",
language = "English",
volume = "10",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Genital Infiltrations of CD4(+) and CD8(+) T Lymphocytes, IgA(+) and IgG(+) Plasma Cells and Intra-Mucosal Lymphoid Follicles Associate With Protection Against Genital Chlamydia trachomatis Infection in Minipigs Intramuscularly Immunized With UV-Inactivated Bacteria Adjuvanted With CAF01

AU - Erneholm, Karin

AU - Lorenzen, Emma

AU - Boje, Sarah

AU - Olsen, Anja Weinreich

AU - Jungersen, Gregers

AU - Jensen, Henrik E.

AU - Cassidy, Joseph P.

AU - Andersen, Peter

AU - Agerholm, Jorgen S.

AU - Follmann, Frank

PY - 2019

Y1 - 2019

N2 - The development of a vaccine against genital chlamydia in women is advancing, and the evaluation of in situ immune responses following vaccination and challenge infections is crucial for development of a safe and protective vaccine. This study employs the sexually mature minipig model to characterize the genital in situ immune response to Chlamydia trachomatis infection in pigs previously immunized intramuscularly with UV-inactivated C. trachomatis serovar D (UV-SvD) adjuvanted/formulated with CAF01 adjuvant compared to a CAF01-alone control group. Pigs immunized with UV-SvD were significantly protected against vaginal challenge with C. trachomatis on day 3 post inoculation and showed significantly higher cervical infiltrations of approximately equal numbers of CD4+ and CD8+ T-cells, and IgG+ and IgA+ plasma cells compared to adjuvant-alone immunized controls. These immunological signatures correspond to findings in mice and are similar to those described in female chlamydia patients. This proves important potential for the pig model in elucidating immunological in situ signatures in future translational research in chlamydia vaccinology.

AB - The development of a vaccine against genital chlamydia in women is advancing, and the evaluation of in situ immune responses following vaccination and challenge infections is crucial for development of a safe and protective vaccine. This study employs the sexually mature minipig model to characterize the genital in situ immune response to Chlamydia trachomatis infection in pigs previously immunized intramuscularly with UV-inactivated C. trachomatis serovar D (UV-SvD) adjuvanted/formulated with CAF01 adjuvant compared to a CAF01-alone control group. Pigs immunized with UV-SvD were significantly protected against vaginal challenge with C. trachomatis on day 3 post inoculation and showed significantly higher cervical infiltrations of approximately equal numbers of CD4+ and CD8+ T-cells, and IgG+ and IgA+ plasma cells compared to adjuvant-alone immunized controls. These immunological signatures correspond to findings in mice and are similar to those described in female chlamydia patients. This proves important potential for the pig model in elucidating immunological in situ signatures in future translational research in chlamydia vaccinology.

KW - porcine

KW - chlamydia

KW - pathology

KW - histology

KW - vaccine

U2 - 10.3389/fmicb.2019.00197

DO - 10.3389/fmicb.2019.00197

M3 - Journal article

C2 - 30800114

VL - 10

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 197

ER -

ID: 216924461